Prominence uflc instrument

Manufactured by Shimadzu

The Prominence UFLC is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. It features a modular design, allowing for the integration of various components such as pumps, detectors, and sample injectors. The Prominence UFLC is capable of performing ultra-fast liquid chromatography (UFLC) analysis, providing efficient and rapid separation of complex samples.

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Lab products found in correlation

Forsythiaside a, by Merck Group (1 mentions) Glycyrrhizic acid, by Merck Group (1 mentions) Qtrap 6500, by AB Sciex (1 mentions)

2 protocols using prominence uflc instrument

HPLC Fingerprinting of Medicinal Herbs

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For analyzing HPLC fingerprint profiles, SAM (10mg/ml) or the marker glycyrrhizic acid, licorice, and forsythiaside A (10 μL) were injected directly into the Prominence UFLC instrument (Shimadzu LC-10A) with a C-18 reverse phase column. glycyrrhizic acid, licorice, and forsythiaside A were purchased from Sigma-Aldrich Chemical Co. (St. Louis, Mo, USA). Separation was conducted with a gradient elution of 0.6% phosphoric acid and acetonitrile (0-30 min, B 98%→70%; 30-60 min, B 70%→39%) at a flow rate of 0.8 mL/min. Chromatographic peaks were detected at 260 nm with a SPD-M20A DAD detector. Chromatographic data were collected and processed by an Empower™ chromatographic working station.

Xiao H., Zhao R., He Y., Liu Y., He Q., Wang S, & Chen F. (2018). Siji Antiviral Mixture Protects against CA16 Induced Brain Injury through Inhibiting PERK/STAT3/NF-κB Pathway. BioMed Research International, 2018, 8475463.

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Polar Metabolite Profiling Workflow

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sNF96.2 and JH-2-002 cells were plated in equal numbers and grown to 70% confluence per 10 cm² plate in media. Cells were treated in quadruplicate with JHU395 (10 μM) or DMSO for 6 hours, then media was aspirated and cells were washed with cold PBS. Polar metabolites were extracted in 2 mL ice cold 80% methanol/20% water following the published protocol of Yuan et. al. [24 (link)]. Dried metabolite pellets were resuspended in 20 uL LC/MS grade water and 5 uL was injected onto an AB/SCIEX 6500 QTRAP hybrid triple quadrupole mass spectrometer targeting >300 polar metabolites via selected reaction monitoring (SRM) with polarity switching. Metabolites were separated using amide XBridge HILIC (Waters Corp.) chromatography with a Shimadzu Prominence UFLC instrument. Data were integrated using MultiQuant 3.0 software to generate peak area intensities. For details of FGAR quantification please see Supplemental Methods.

Wexler H.M., Molitoris D, & Finegold S.M. (2000). In Vitro Activities of MK-826 (L-749,345) against 363 Strains of Anaerobic Bacteria. Antimicrobial Agents and Chemotherapy, 44(8), 2222-2224.

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