Anti vav1

Manufactured by Cell Signaling Technology

Anti-Vav1 is a primary antibody that specifically binds to the Vav1 protein. Vav1 is a guanine nucleotide exchange factor involved in signal transduction pathways. This product can be used for the detection and analysis of Vav1 in various research applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd28 antibody, by R&D Systems (1 mentions) Anti cox 4, by Cell Signaling Technology (1 mentions) Odyssey clx imaging system, by LI COR (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Ecl system, by GE Healthcare (1 mentions) Sirt1, by Cell Signaling Technology (1 mentions) Anti phosphotyrosine, by Merck Group (1 mentions) Anti slp 76, by Cell Signaling Technology (1 mentions) Anti lat, by Cell Signaling Technology (1 mentions) Anti zap70, by Cell Signaling Technology (1 mentions)

3 protocols using anti vav1

Immunoblot Analysis of Cellular Proteins

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblots were performed as previously described using the Odyssey CLx
Imaging System (Li-Cor, Lincoln, NE) (32 (link)). The following primary antibodies were from Cell Signaling
Technologies: anti-GAPDH, anti-Vav1, and anti-COX IV. The anti-CD28 antibody was
purchased from R&D Systems (Minneapolis, MN).

Helou Y.A., Petrashen A.P, & Salomon A.R. (2015). Vav1 regulates T cell activation through a feedback mechanism and crosstalk between the T cell receptor and CD28. Journal of proteome research, 14(7), 2963-2975.

+ Open protocol

+ Expand

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Twenty micrograms of cell lysate were analyzed on a denaturing 4%-15%
gradient polyacrylamide gel (Biorad, 567–1081, www.bio-rad.com), transferred onto a PVDF membrane (Millipore,
IPVH08100, www.merckmillipore.com) and incubated with various antibodies
purchased from Cell Signaling Technology, which include anti-Vav1 (Cat. 4657),
Sirt1 (Cat. 3931), Sirt1 (Cat. 8469S, for human), PPARγ (Cat. 2443),
C/EBPα (Cat. 2295), Creb (Cat. 9197S), phospho-Creb (Cat. 9191S), and
acetylated lysine (Cat. 9441). Antibody against Col2α1 was purchased from
Santa Cruz Biotechnology (Cat. sc-52658). All antibodies were used at 1:500
dilution unless otherwise specified. Immune membranes were visualized using
horseradish peroxidase-conjugated secondary antibodies (Cell signaling, Cat 7074
and 7076, www.cellsignal.com) and a chemiluminescent
substrate (ECL system, GE Healthcare, www.gelifesciences.com).

Qu P., Wang L., Min Y., McKennett L., Keller J.R, & Lin P.C. (2016). Vav1 Regulates Mesenchymal Stem Cell Differentiation Decision Between Adipocyte and Chondrocyte via Sirt1. Stem cells (Dayton, Ohio), 34(7), 1934-1946.

+ Open protocol

+ Expand

Immunoblot analysis of T-cell signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Affinity-purified samples and whole-cell lysates were loaded on 8–10% SDS-PAGE gel and subsequently analyzed by immunoblot with specific antibodies. The following antibodies were used for immunoblot analysis: anti–ZAP-70 (2705; Cell Signaling Technology), anti-LAT (9166; Cell Signaling Technology), anti–SLP-76 (4958; Cell Signaling Technology), anti-VAV1 (2502; Cell Signaling Technology), and anti-phosphotyrosine (4G10; Millipore).

Nicolas P., Ollier J., Mori D., Voisinne G., Celis-Gutierrez J., Gregoire C., Perroteau J., Vivien R., Camus M., Burlet-Schiltz O., Gonzalez de Peredo A., Clémenceau B., Roncagalli R., Vié H, & Malissen B. (2022). Systems-level conservation of the proximal TCR signaling network of mice and humans. The Journal of Experimental Medicine, 219(2), e20211295.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!