Specific Ab responses were measured by conventional ELISA. Briefly, ELISA plates were coated overnight at room temperature with FTAg (15 μg/ml). A serial dilution of the sera was carried out to determine the titer, which is defined as the inverse of the highest serum dilution factor giving an absorbance of >0.2. The Ab titers were determined using the following HRP-conjugated secondary Abs: rabbit anti-mouse IgG (H+L)–HRP, rabbit anti-mouse IgG1–HRP, and rabbit anti-mouse IgG2a–HRP (South-ernBiotech, Birmingham, AL; all with 1:1000 dilutions). SureBlue (KPL, Gaithersburg, MD) was used as a peroxidase substrate. After 15 min, the reaction was stopped by the addition of 100 μl 1 M H2SO4 and the absorbance was read at 450 nm.
Rabbit anti mouse igg2a hrp
Manufactured by Southern Biotech
Rabbit anti-mouse IgG2a–HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to mouse IgG2a antibodies in various immunoassays and research applications.
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Lab products found in correlation
2 protocols using rabbit anti mouse igg2a hrp
1
FTAg-Specific Antibody Titration
2
ELISA for Measuring IgG Antibodies
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The IgG specific Ab responses were measured by conventional ELISA method. Briefly, ELISA plates were coated overnight at room temperature with FTAg (80 μg/ml). A serial dilution of the sera was carried out to determine the titer, which is defined as the inverse of the highest serum dilution factor giving an absorbance of >0.2. Titers for the Abs were determined using the following HRP-conjugated secondary Abs: Rabbit anti-mouse IgG (H+L)–HRP, Rabbit anti-mouse IgG1-HRP, Rabbit anti-mouse IgG2a-HRP (Southern Biotech; all with 1:1000 dilutions). SureBlue (KPL) was used as a peroxidase substrate. After 15 min, the reaction was stopped by the addition of 100 μl 1M H2SO4, and the absorbance was read at 450 nm.
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