2 mercaptoethanol

Manufactured by Merck Group

Sourced in United States, Germany, United Kingdom, Japan, France, Canada, Australia, China, Italy, Sao Tome and Principe, Switzerland, Denmark, Austria, Macao, Poland, Spain, Israel, Ireland, Gabon, Belgium, Czechia, Sweden, Argentina

2-mercaptoethanol is a clear, colorless liquid that is commonly used as a reducing agent in various laboratory applications. It is a small organic molecule with the chemical formula C2H6OS. 2-mercaptoethanol plays a crucial role in maintaining the proper conformation and disulfide bond formation of proteins during sample preparation and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Mercaptoethanol (160 citations) 2-β-mercaptoethanol (88 citations) 2-mercaptoethanol (2-ME) (44 citations) 2-mercaptoethanol βME (13 citations) 2-mercaptoethanol (BME) (9 citations) M 2-mercaptoethanol (9 citations) 2-mercaptoethanol (M3148) (3 citations) 2-Mercaptoethanol (ME), (3 citations) EmbryoMax 2-Mercaptoethanol (3 citations) β-mercaptoethanol (2-ME) (2 citations)

1 795 protocols using 2 mercaptoethanol

Reprogramming Protocols for 293T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

293T cells were grown in Dulbecco’s modified eagle medium (DMEM) with 1% Penicillin/Streptomycin (5000U/ml), 2mM L-glutamine (all from GIBCO) and 10% fetal bovine serum (FBS, Sigma). Referred to herein as 293T medium.
MEFs were obtained from embryos at day E12.5 and grown in DMEM with 1% Penicillin/Streptomycin (5000U/ml), 2mM L-glutamine, 10%–20% FBS and 0.1mM 2-mercaptoethanol (Sigma). MEFs were used prior passage 2 for all reprogramming studies.
MEFs undergoing somatic reprogramming were grown in iPSC medium: DMEM with 1% Penicillin/Streptomycin (5000U/ml), 2mM L-glutamine, 5% ESC qualified FBS (Hyclone, Fisher Scientific), 1% non-essential amino acids (GIBCO), 0.1mM 2-mercaptoethanol and 0.01% LIF (Leukemia inhibitory factor, 10⁶U/ml, Millipore).

Ward C., Volpe G., Cauchy P., Ptasinska A., Almaghrabi R., Blakemore D., Nafria M., Kestner D., Frampton J., Murphy G., Buganim Y., Kaji K, & García P. (2018). Fine-Tuning Mybl2 Is Required for Proper Mesenchymal-to-Epithelial Transition during Somatic Reprogramming. Cell Reports, 24(6), 1496-1511.e8.

+ Open protocol

+ Expand

Expansion Media for Tregs and T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tregs and T cells were cultured in Expansion Media, DMEM-based media (DMEM, high glucose, pyruvate; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS; Premium; Atlanta Biologicals), 10 mM HEPES (MilliporeSigma), 1× nonessential amino acids (NEAA; Thermo Fisher Scientific), 1× penicillin/streptomycin (MilliporeSigma), 50 μg/mL gentamicin (gentamicin sulfate, liquid, Corning), and 55 mM 2-mercaptoethanol (MilliporeSigma). TBL12 and hCD19 TBL12^luc cell lines were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific), 10 mM HEPES (MilliporeSigma), 1× penicillin/streptomycin (MilliporeSigma), 1× NEAA (Fischer Scientific), and 55 mM 2-mercaptoethanol (MilliporeSigma).

Bolivar-Wagers S., Loschi M.L., Jin S., Thangavelu G., Larson J.H., McDonald-Hyman C.S., Aguilar E.G., Saha A., Koehn B.H., Hefazi M., Osborn M.J., Jensen M.C., Wagner J.E., Pennell C.A, & Blazar B.R. (2022). Murine CAR19 Tregs suppress acute graft-versus-host disease and maintain graft-versus-tumor responses. JCI Insight, 7(17), e160674.

+ Open protocol

+ Expand

Protein Extraction and Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

All solvents used were of HPLC-grade and purchased from Sigma-Aldrich, Poole, UK, except water, which was acquired through Fisher Scientific, Loughborough, UK. The isolation buffer consisted of 200 mM sorbitol (Fluka Biochemika, Buchs, Switzerland), 2 mM sucrose (Sigma-Aldrich), 5 mM succinic acid (Sigma-Aldrich), 5 mM dithiothreitol (Sigma-Aldrich), 1 mM ethylene glycol-bis(2-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (Sigma-Aldrich), 0.5 mM Na₂HPO₄ (Sigma-Aldrich), 0.1 mM Na₄P₂O₇ (Sigma-Aldrich), 25 mM HEPES (Sigma-Aldrich) and 5 mM MgCl₂ (Sigma-Aldrich) in water. The precipitation solution was made up with 10 % (w/v) trichloroacetic acid (Fiedel-de Haen, Buchs, Switzerland) and 0.07 % (w/v) 2-mercaptoethanol (Sigma-Aldrich) in cold acetone (Sigma-Aldrich) while the rinsing solution incorporated just 0.07 % (w/v) 2-mercaptoethanol in cold acetone. The solubilization solution consisted of 7 M urea (Sigma-Aldrich) and 2 M thiourea (Sigma-Aldrich) in water.

Bryant L., Flatley B., Patole C., Brown G.D, & Cramer R. (2015). Proteomic analysis of Artemisia annua – towards elucidating the biosynthetic pathways of the antimalarial pro-drug artemisinin. BMC Plant Biology, 15, 175.

+ Open protocol

+ Expand

Culturing Mouse Embryonic Stem Cells and Drosophila S2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Undifferentiated mouse E14 ES cells (gift from Huck-Hui Ng) were cultured under feeder-free conditions. ES cells were seeded on gelatin-coated dishes and were cultured in DMEM medium (GIBCO) supplemented with 15% fetal bovine serum (FBS; Gemini Gemcell), 0.055 mM 2-mercaptoethanol (Sigma), 2 mM Glutamax (GIBCO), 0.1 mM MEM non-essential amino acid (GIBCO), 5,000 U ml⁻¹ penicillin/streptomycin (GIBCO) and 1,000 U ml⁻¹ of LIF (Millipore). The cells were maintained in an incubator at 37 °C and 5% CO₂.
MEFs (C57BL/5, GlobalStem) were cultivated in 15-cm dishes in DMEM (GIBCO) supplemented with 15% FBS (Gemini Gemcell), 0.055 mM 2-mercaptoethanol (Sigma), 2 mM Glutamax (GIBCO), 0.1 mM MEM non-essential amino acid (GIBCO), 5,000 U ml⁻¹ penicillin/streptomycin (GIBCO). MEFs were also maintained in an incubator at 37 °C and 5% CO₂.
Drosophila S2 cells (Invitrogen) were maintained in 15-cm plates in Schneider's Drosophila Medium (GIBCO) supplemented with 10% heat-inactivated FBS (Gemini Gemcell) and 5 ml 1:100 penicillin/streptomycin (GIBCO) in an incubator at 28 °C without CO₂.

Nguyen T.C., Cao X., Yu P., Xiao S., Lu J., Biase F.H., Sridhar B., Huang N., Zhang K, & Zhong S. (2016). Mapping RNA–RNA interactome and RNA structure in vivo by MARIO. Nature Communications, 7, 12023.

+ Open protocol

+ Expand

Protein Extraction and Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded into 12-well tissue culture plates (Corning). After 24 h, cells were stimulated with LPS (O55:B5, Sigma-Aldrich) for 6 h; during the last 2 h of LPS stimulation, nigericin (InvivoGen) was added. Total protein lysates were prepared as previously described [41 (link)]; 4 × Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA) with 10% 2-mercaptoethanol (Sigma-Aldrich) was added, and samples were frozen at −20 °C for subsequent immunoblot analysis. To extract proteins from cell supernatants, protein-binding StrataClean resin (Agilent, Santa Clara, CA, USA) was added; the mixture was vortexed for 30 s and subsequently spun (5 min, 250× g, 4 °C). StrataClean resin pellets were resuspended in 4× Laemmli sample buffer (Bio-Rad Laboratories) with 10% 2-mercaptoethanol (Sigma-Aldrich), and stored at −20 °C until being subjected to immunoblot analysis.

Rudloff I., Ung H.K., Dowling J.K., Mansell A., D’Andrea L., Ellisdon A.M., Whisstock J.C., Berger P.J., Nold-Petry C.A, & Nold M.F. (2020). Parsing the IL-37-Mediated Suppression of Inflammasome Function. Cells, 9(1), 178.

+ Open protocol

+ Expand

Derivation of Mouse Embryonic Fibroblasts and Thymic Lymphoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse embryonic fibroblasts (MEFs) were derived as previously described (Putkey et al. 2002 (link)). Briefly, E13.5 embryos were washed with PBS, and head, liver, and tail were removed. Embryos were minced in 0.05% trypsin (Gibco) and incubated for 15 min at 37°C. Dissociated cells were plated in DMEM media (Gibco) supplemented with 15% fetal bovine serum (Omega Scientific), 50 µg/mL penicillin and streptomycin (Gibco), 2 mM L-glutamine (Gibco), 1 µM 2-mercaptoethanol (Sigma), 1 mM sodium pyruvate (Gibco), and 0.1 mM nonessential amino acids (Gibco). Cells were maintained at 37°C, 5% CO₂, and 3% O₂. Doxycycline (Sigma) was dissolved in water and used at a final concentration of 1 μg/mL. Thymic lymphoma cells were derived as previously described (Jinadasa et al. 2011 (link)). Briefly, dissected tumors were dissociated using bent needles, and cells were plated in RPMI 1640 with 25 mM HEPES and 200 mM L-glutamine (Lonza), and supplemented with 10% fetal bovine serum (Hyclone), 1% penicillin and streptomycin (Gibco), 1% nonessential amino acids (Gibco), and 55 mM 2-mercaptoethanol (Sigma).

Shoshani O., Bakker B., de Haan L., Tijhuis A.E., Wang Y., Kim D.H., Maldonado M., Demarest M.A., Artates J., Zhengyu O., Mark A., Wardenaar R., Sasik R., Spierings D.C., Vitre B., Fisch K., Foijer F, & Cleveland D.W. (2021). Transient genomic instability drives tumorigenesis through accelerated clonal evolution. Genes & Development, 35(15-16), 1093-1108.

+ Open protocol

+ Expand

Rcho-1 Cell Maintenance and Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rcho-1 cells were kindly provided by Professor Michael J. Soares (Department of Physiology, University of Kansas Medical Center, Kansas City, KS). Protocols for the maintenance and differentiation of Rcho-1 cells were in accordance with previous work16 (link). The cells were routinely cultured in standard growth medium (RPMI1640, Sigma-Aldrich, St. Louis, MO) containing 50 μM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), 1 mM sodium pyruvate (Wako, Osaka, Japan), and 20% fetal bovine serum. Rcho-1 cell differentiation was induced by culturing in NCTC-135 culture medium (Sigma-Aldrich, St. Louis, MO) containing 50 μM 2-mercaptoethanol, 1 mM sodium pyruvate, and 1% horse serum. For observation of Rcho-1 cell shape, we used CellMask (Thermo Fisher Scientific, Tokyo, Japan) according to the manufacturer’s protocol.

Arikawa T., Liao S., Shimada H., Inoue T., Sakata-Haga H., Nakamura T., Hatta T, & Shoji H. (2016). Galectin-4 expression is down-regulated in response to autophagy during differentiation of rat trophoblast cells. Scientific Reports, 6, 32248.

+ Open protocol

+ Expand

Cell Culture Conditions for Murine Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hepa1‐6 cells were maintained in DMEM (Wako Pure Chemical Industries) supplemented with 10% FBS (Nichirei Bioscience Inc., Tokyo, Japan), 200 U/mL penicillin, 100 μg/mL streptomycin, and 10 mM HEPES (Sigma‐Aldrich Japan) at 37°C in a humidified atmosphere containing 5% CO₂. CT26 cells were maintained in RPMI‐1640 medium (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 10% FBS, 200 U/mL penicillin, 100 μg/mL streptomycin, 10 mM HEPES, and 0.05 mmol/L 2‐mercaptoethanol (Sigma‐Aldrich) at 37°C in a humidified atmosphere containing 5% CO₂. EG7‐OVA cells were maintained in RPMI‐1640 medium (Wako Pure Industries, Ltd.) supplemented with 10% FBS, 200 U/mL penicillin, 100 μg/mL streptomycin, 10 mM HEPES, 0.05 mmol/L 2‐mercaptoethanol (Sigma‐Aldrich) and 100 μg/mL G418 (Wako Pure Industries, Ltd.) at 37°C in a humidified atmosphere containing 5% CO₂. All cell lines were tested to rule out the presence of mycoplasma contamination.

Shen W., Wang X., Xiang H., Shichi S., Nakamoto H., Kimura S., Sugiyama K., Taketomi A, & Kitamura H. (2023). IFN‐γ–STAT1‐mediated NK2R expression is involved in the induction of antitumor effector CD8 + T cells in vivo. Cancer Science, 114(5), 1816-1829.

+ Open protocol

+ Expand

Murine Embryonic Stem Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were grown under two different culture conditions, prepared as follows:
2i/2iLIF: serum-free KSR (knockout serum replacement) 10% (Life Technologies, cat. 10828-028) - based medium in GMEM (Sigma-Aldrich, cat. G5154) supplemented with 1% FBS (Sigma-Aldrich, cat. F7524), 100 mM 2- mercaptoethanol (Sigma-Aldrich, cat. M7522), 1× MEM non-essential amino acids (Invitrogen, cat. 1140-036), 2 mM L-glutamine, 1 mM sodium pyruvate (both from Invitrogen), and with small-molecule inhibitors PD (1 μM, PD0325901), CH (3 mM, CHIR99021) from Axon (cat. 1386 and 1408) and LIF (100 units/ml, produced in-house).
Serum LIF: GMEM (Sigma-Aldrich, cat. G5154) supplemented with 10% FBS (Sigma-Aldrich, cat. F7524), 100 mM 2-mercaptoethanol (Sigma-Aldrich, cat. M7522), 1× MEM non-essential amino acids (Invitrogen, cat. 1140-036), 2 mM L-glutamine, 1 mM sodium pyruvate (both from Invitrogen), and 100 units/ml LIF.

Betto R.M., Diamante L., Perrera V., Audano M., Rapelli S., Lauria A., Incarnato D., Arboit M., Pedretti S., Rigoni G., Guerineau V., Touboul D., Stirparo G.G., Lohoff T., Boroviak T., Grumati P., Soriano M.E., Nichols J., Mitro N., Oliviero S, & Martello G. (2021). Metabolic control of DNA methylation in naïve pluripotent cells. Nature genetics, 53(2), 215-229.

+ Open protocol

+ Expand

Murine Embryonic Stem Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!