Trizma® hydrochloride (Tris) (Merck, Dorset, U.K.) (10812846001), Sodium chloride (NaCl) (Merck, Dorset, U.K.) (71383), DPBS (Merck, Dorset, U.K.) (D8537), Sodium deoxycholate (Merck, Dorset, U.K.) (D6750), Triton 100 (Merck, Dorset, U.K.) (×100), Protease inhibitor cocktail (Merck, Dorset, U.K.) (P8340).
Trizma hydrochloride
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, Cameroon
Trizma hydrochloride is a chemical compound commonly used as a buffer in various laboratory applications. It functions as a pH-regulating agent, helping to maintain a specific pH environment in experimental solutions or processes.
Automatically generated - may contain errors
Lab products found in correlation
Trizma base, by Merck Group (39 mentions)
Sodium chloride (nacl), by Merck Group (21 mentions)
Bovine serum albumin (bsa), by Merck Group (11 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (9 mentions)
Sodium deoxycholate, by Merck Group (8 mentions)
Urea, by Merck Group (7 mentions)
Sodium hydroxide (naoh), by Merck Group (7 mentions)
Iodoacetamide, by Merck Group (6 mentions)
Mgcl2, by Merck Group (6 mentions)
Hydrochloric acid (hcl), by Merck Group (6 mentions)
Potassium chloride (kcl), by Merck Group (5 mentions)
Tween 20, by Merck Group (5 mentions)
Formic acid, by Thermo Fisher Scientific (5 mentions)
Glacial acetic acid, by Merck Group (5 mentions)
Formic acid, by Merck Group (5 mentions)
Triton x 100, by Merck Group (5 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (5 mentions)
Dl dithiothreitol, by Merck Group (4 mentions)
Sucrose, by Merck Group (4 mentions)
Citric acid, by Merck Group (4 mentions)
87 protocols using trizma hydrochloride
1
Protein Extraction Buffer Preparation
2
Oligo Purification and Enzyme Preparation
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HPLC-purified
oligonucleotides were purchased
from Biomers or Eurofins and resuspended at 100 μM in 1×
tris–EDTA at pH 7.5 for long-term storage. The nicking enzymes
Nb.BsmI and Nt.bstNBI, the restriction enzyme BsmI, the DNA polymerase
Vent(exo-), BSA, and dNTP were obtained from New England Biolabs (NEB). Thermus thermophilus RecJ exonuclease was produced
in-house by following a previously published protocol.37 (link) Sodium chloride, potassium chloride, magnesium
sulfate, ammonium sulfate, Trizma hydrochloride, netropsin, and synperonic
F104 were purchased from Merck (Sigma-Aldrich).
oligonucleotides were purchased
from Biomers or Eurofins and resuspended at 100 μM in 1×
tris–EDTA at pH 7.5 for long-term storage. The nicking enzymes
Nb.BsmI and Nt.bstNBI, the restriction enzyme BsmI, the DNA polymerase
Vent(exo-), BSA, and dNTP were obtained from New England Biolabs (NEB). Thermus thermophilus RecJ exonuclease was produced
in-house by following a previously published protocol.37 (link) Sodium chloride, potassium chloride, magnesium
sulfate, ammonium sulfate, Trizma hydrochloride, netropsin, and synperonic
F104 were purchased from Merck (Sigma-Aldrich).
3
Cannabinoid Receptor Binding Assay
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CP55,940 was purchased from Tocris Bioscience (Minneapolis, MN, USA). BSA, TrizmaTM hydrochloride (Tris-HCl), penicillin and streptomycin, nonenzymatic cell dissociation solution, and guanosine 5′-diphosphate (GDP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). [3H]-CP55,940 and MicroScintTM-20 were purchased from PerkinElmer (Waltham, MA, USA). Membrane preparation was made using a 50 mM Tris-HCl buffer with pH 7.4. Dilutions of membrane, radioligand and control/test compounds were made in a Tris-EDTA buffer (50 mM Tris-HCl, 20 mM EDTA, 154 mM NaCl, and 0.2% fatty-acid BSA), with pH = 7.4.
4
Cannabinoid Receptor Binding Assay
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CP55,940 was purchased from Tocris Bioscience (Minneapolis, MN, USA). BSA, TrizmaTM hydrochloride (Tris-HCl), penicillin, streptomycin, and nonenzymatic cell dissociation solution were purchased from Sigma-Aldrich (St. Louis, MO, USA). Radioligands, GF/C, GF/B 96-well plates, and MicroScintTM-20, were purchased from PerkinElmer (Waltham, MA, USA). Membrane preparation was made using a 50 mM Tris-HCl buffer with pH 7.4. Tetrahydromagnolol (4 ) was purchased from Sigma-Aldrich (St. Louis, MO, USA) (% purity ≥ 95).
5
Reagents and Oligonucleotides for Telomere Assay
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Potassium
hexacyanoferrate(III) (99.98%),
potassium hexacyanoferrate(II) trihydrate (≥99.95%), ethylenediaminetetraacetic
acid (EDTA) ACS reagent (99.4–100.6%), 2-propanol anhydrous
(99.5%), sodium chloride (≥99.95%), acetic acid (≥99%),
Tween 20, Trizma base (99.9%) Trizma hydrochloride (≥99%),
methanol (≥99%), phenylmethanesulfonyl fluoride (PMSF), diethyl
pyrocarbonate (DCEP) (≥97%),dl -dithiothreitol (DTT)
(≥98%), glycine (≥99%), sodium dodecyl sulfate (SDS;
≥98.5%), and ddGTPs were purchased from Sigma-Aldrich (St.
Louis, MO). Acrylamide (40%), ammonium persulfate, tetramethylethylenediamine,
4× Laemmli sample buffer, and precision plus protein standard
were obtained from BioRad (Hercules, CA). Tris(2-carboxyethyl) phosphine
hydrochloride (TCEP), sulfuric acid optima (93–98%), Coomassie
Brilliant Blue G-250, HEPES buffer, detergent-compatible Bradford
assay kit, and RPMI-164 medium + 2.05 mM glutamine (Hyclone) were
purchased from Fisher Scientific (Fair-lawn, NJ). All synthetic oligonucleotides
were purchased from Integrated DNA Technology (IDT) (San Diego, CA)
with the following sequences:
TS30 (5′-S-S-(CH2)6-TTTTTTTTTTAATCCGTCGAGCAGAGTT-3′),
TS60 (5′-S-S-(CH2)6-TTTTTTTTTTAATCCGTCGAGCAGAGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG-3′),
and
TSC (5′-S-S-(CH2)6-AAAAAAAAAATTAGGCAGCTCGTCTCAA-3′).
dNTPs were purchased
from Promega (Madison, WI, USA).
hexacyanoferrate(III) (99.98%),
potassium hexacyanoferrate(II) trihydrate (≥99.95%), ethylenediaminetetraacetic
acid (EDTA) ACS reagent (99.4–100.6%), 2-propanol anhydrous
(99.5%), sodium chloride (≥99.95%), acetic acid (≥99%),
Tween 20, Trizma base (99.9%) Trizma hydrochloride (≥99%),
methanol (≥99%), phenylmethanesulfonyl fluoride (PMSF), diethyl
pyrocarbonate (DCEP) (≥97%),
(≥98%), glycine (≥99%), sodium dodecyl sulfate (SDS;
≥98.5%), and ddGTPs were purchased from Sigma-Aldrich (St.
Louis, MO). Acrylamide (40%), ammonium persulfate, tetramethylethylenediamine,
4× Laemmli sample buffer, and precision plus protein standard
were obtained from BioRad (Hercules, CA). Tris(2-carboxyethyl) phosphine
hydrochloride (TCEP), sulfuric acid optima (93–98%), Coomassie
Brilliant Blue G-250, HEPES buffer, detergent-compatible Bradford
assay kit, and RPMI-164 medium + 2.05 mM glutamine (Hyclone) were
purchased from Fisher Scientific (Fair-lawn, NJ). All synthetic oligonucleotides
were purchased from Integrated DNA Technology (IDT) (San Diego, CA)
with the following sequences:
and
dNTPs were purchased
from Promega (Madison, WI, USA).
6
DMEM-based Cell Culture Protocol
7
Analytical Chemistry Reagents Protocol
8
Synthesis of PEGylated Nanoparticles
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Oleic acid (technical grade 90%), Iron (III) acetylacetonate (97%), Sodium chloride (99%), Dihexadecyl phosphate (90%), 1-Octadecene (90%), 2-Butanol (95.5%), Trizma® hydrochloride (99%), Calcium chloride (97%), Magnesium sulfate (99.5%), Glycine (99%), Glycerol (99%), Urea (98%), 2-(N-Morpholino)ethanesulfonic acid (99%), Sucrose (99.5%), Sigma-Aldrich (Poznan, Poland). Toluene (99.5%), n-Hexane (99%), Chloroform (98.5%) and Hydrochloric acid (30–35%), Avantor (Gliwice, Poland). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy-(polyethyleneglycol)-2000] (ammonium salt) (PL-PEG-COOH, 2000 Da PEG (99%), Avanti, Alabaster, AL, USA). Snakeskin® Dialysis Tubing, 10K MWCO, 22 mm, Thermo Fisher Scientific (Waltham, MA, USA). All chemicals were used as received. Water was purified with Hydrolab HLP5 instrument (0.09 μS/cm, Straszyn, Poland).
9
Quantification of H2S in Renal Plasma and Tissue
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All chemicals used for liquid chromatography (LC) and mass spectrometry (MS) experiments were of LC‐MS grade. Trypsin Gold was purchased from Promega. Urea, sodium deoxycholate (DOC), Trizma hydrochloride (Tris), DL‐dithiothreitol (DTT), iodoacetamide (IAA), N‐acetyl‐L‐cysteine (NAC), ethylenediaminetetraacetic acid (EDTA), bovine serum albumin (BSA), ammonium bicarbonate and all solvents were provided by Sigma‐Aldrich, unless otherwise specified. Complete protease inhibitor cocktail was offered by Roche. C18 solid‐phase extraction (SPE) columns were acquired from Waters Corporation. Protein concentration was determined by amido black assay using Amido Black 10B (JT Baker Chemical Co.). Cholesterol, glucose and triglyceride plasma levels were determined using the specific assays from DIALAB GMBH. The concentration of renal plasma and renal tissue H2S were determined by the H2S Assay Kit E‐BC‐K355 (Elabscience) according to the manufacturer's instructions.
10
Production of H-1PV Virus in NB-324K Cells
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H-1PV wild-type (wt) virus was produced in a 10-layer CellSTACK® (CS; Corning, Wiesbaden, Germany) according to Leuchs et al. 2016 (link). For this, NB-324K cells were seeded at 3.6E4 cells/cm2 and infected immediately with H-1PV wt (Kestler et al. 1999 (link)) at a multiplicity of infection (MOI) of 0.01 plaque forming units (PFU) per cell. To generate the clarified cell lysate, the cells were harvested after 4 days of incubation, lysed in 50 mM Tris-HCl (Trizma® hydrochloride; Sigma-Aldrich Co, St. Louis, USA) buffer, DNAse-treated (50 U/ml, Sigma-Aldrich Chemie GmbH, Steinheim, Germany), and filtered through 0.2- and 0.1-μm membranes (Sartorius AG, Göttingen, Germany).
The H-1PV is also publically available at ATCC® VR-356.
The H-1PV is also publically available at ATCC® VR-356.
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