Ez cytox assay kit

Manufactured by Daeil Lab Service

Sourced in Cameroon

The EZ-Cytox assay kit is a colorimetric cell viability and cytotoxicity detection reagent. It provides a simple and rapid method to quantify the number of viable cells in a given sample.

Automatically generated - may contain errors

Lab products found in correlation

Microplate reader, by Tecan (6 mentions) 96 well flat bottom culture plates, by SPL Life Sciences (3 mentions) Versamax elisa microplate reader, by Molecular Devices (3 mentions) Synergy h2, by Agilent Technologies (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Synergy h1 multi mode reader, by Agilent Technologies (1 mentions) Elisa reader, by Tecan (1 mentions) Microplate reader, by Molecular Devices (1 mentions) Elisa plate reader, by Molecular Devices (1 mentions) Synergy h1 hybrid multi mode reader, by Agilent Technologies (1 mentions) Versamax, by Molecular Devices (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Llc pk1 cells, by American Type Culture Collection (1 mentions) Microplate spectrophotometer, by Bio-Rad (1 mentions) Synergy h1 plate reader, by Agilent Technologies (1 mentions) Spark 10m, by Tecan (1 mentions) Edu cell proliferation assay kit, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Bio-Rad (1 mentions) Fluostar omega, by BMG Labtech (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

30 protocols using ez cytox assay kit

Cytotoxicity of Lithospermi Radix Extract

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Example 5

Cytotoxicity of the Lithospermi Radix extract in human foreskin fibroblast cells was determined to see whether it is toxic to normal cells.

Specifically, human foreskin fibroblast cells were cultured according to [Materials and Methods 3-2]. The cells were plated in a 96-well culture dish at 2,000 cells per well. After 24 hours, the cells were treated with serially diluted Lithospermi Radix extract prepared according to [Materials and Methods 1] at final concentrations of 0 to 1000 μg/ml. After 48 hours, cell viability (%) was measured using Ez-Cytox assay kit (Daeillab Service Co., Ltd.). The relative cell viability (%) was calculated compared with the control group which was not treated with a Lithospermi Radix extract.

As shown in FIG. 7, the human foreskin fibroblast cells maintained their viability over 95% even when the cells were treated with a Lithospermi Radix extract at the maximum concentration of 1,000 μg/ml. Therefore, it was confirmed that the Lithospermi Radix extract is not toxic to normal cells (FIG. 7).

US10517913B2. Composition for prevention, alleviation, or treatment of peripheral neuropathy comprising Lithospermi Radix extract as an effective component (2019-12-31). KOREA INSTITUTE OF ORIENTAL MEDICINE [KR]. Inventors: No Soo Kim [KR], Jong-Shik Park [KR], Jin-Mu Yi [KR], You Jin Lee [KR], Eun-Sang Cho [KR], Jinhee Kim [KR], Chae Jun Lim [KR], Ok-Sun Bang [KR], Youngah Kim [KR].

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Cytotoxicity Assay of FSCP in HaCaT Cells

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HaCaT cells (1 × 10⁴ cells/well) in 96-well flat-bottom culture plates (SPL Life Sciences, Pocheon, Korea) were treated with indicated doses of FSCP for 24 h with or without CoCl₂. Cell viability was determined using the colorimetric WST-1 conversion assay (EZ-Cytox assay kit, Daeil Lab Service, Seoul, Korea). WST-1 reagent (10 μL) was added to each well, after which cells were incubated for 2 h in a humidified incubator at 37°C under 5% CO₂. Absorbance of the formazan dye, generated by the reaction between dehydrogenase and WST-1 in metabolically active cells, was measured using a microplate reader (Tecan, Männedorf, Switzerland) at 450 nm according to the manufacturer's instructions. Percent cell viability was calculated. Experiments were performed at least thrice.

Subhan F., Kang H.Y., Lim Y., Ikram M., Baek S.Y., Jin S., Jeong Y.H., Kwak J.Y, & Yoon S. (2017). Fish Scale Collagen Peptides Protect against CoCl2/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells. Oxidative Medicine and Cellular Longevity, 2017, 9703609.

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Cell Proliferation Assay for DPCs

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The changes in cell number were assessed using an EZ-Cytox assay kit (Daeil Lab Service, Korea) according to the manufacturer’s instructions. Human DPCs were seeded at a density of 7000 cells per well in 48-well plates. After attachment, the cells were treated with each test material for 3 days. Medium was replaced with supplement-free CnT basal medium with 20 μl/well of EZ-Cytox solution. After 2 h, the absorbance was measured at 450 nm using a Synergy H1 Multi-Mode Reader (Biotek, USA). The optical density (OD) of each well was used to calculate the cell number based on the authentic standard curves.

Lee E.Y., Nam Y.J., Kang S., Choi E.J., Han I., Kim J., Kim D.H., An J.H., Lee S., Lee M.H, & Chung J.H. (2020). The local hypothalamic–pituitary–adrenal axis in cultured human dermal papilla cells. BMC Molecular and Cell Biology, 21, 42.

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Cell Viability Assay of CRISPR-Olig2

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Cell viability was examined by Ez-Cytox assay kit (Daeil Lab Service Co Ltd, Seoul, Korea). Cells were seeded at 1 × 10⁴ cells/well in a 96-well plate. Cell viability was detected at 24 h, 48 h and 72 h after transfection of CRISPR-Olig2. 10 μl of Ez-Cytox solution was added to each well and 96-well plates were incubated for an additional 1 h at 37 °C in a 5% CO₂ incubator. Cell viability was detected at 490 nm by ELISA reader (Tecan, Mannedorf, Switzerland). Relative cytotoxicity was measured by expressing the viability of the cells as a percentage.

Lee J.E., Ahn S., Jeong H., An S., Myung C.H., Lee J.A., Hong S.C., Kim Y.J., Kim J.Y., Ryu J.H., Noh M., Nam K.T, & Hwang J.S. (2021). Olig2 regulates p53-mediated apoptosis, migration and invasion of melanoma cells. Scientific Reports, 11, 7778.

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Cell Viability Assay using EZ-Cytox

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The cells and medium from each group were used for cell viability with the EZ-Cytox assay kit (Daeil Lab Service, Seoul, Republic of Korea) by adding tetrazolium salt to each group. The samples were then incubated for 3 hours within dark environment. After 3 hours later, the medium from each sample was collected and moved to 96-well plate for measurement. A wavelength of 450 nm absorbance was measured by VersaMax ELISA Microplate Reader (Molecular Devices, CA, USA). Experiment was independently repeated three times.

Han H.W., Hahn S., Jeong H.Y., Jee J.H., Nam M.O., Kim H.K., Lee D.H., Lee S.Y., Choi D.K., Yu J.H., Min S.H, & Yoo J. (2018). LINCS L1000 dataset-based repositioning of CGP-60474 as a highly potent anti-endotoxemic agent. Scientific Reports, 8, 14969.

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Ginsenoside Rh3 Cytotoxicity Assay

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The SP-1 keratinocytes were cultured and placed in 96-well plates (1.0 × 10⁴ cells/well). After 24 h, the cells were treated with ginsenoside Rh3 (1, 10, 100, and 1000 μM) and incubated for 24 h. Then, cell viabilities were determined using an EZ-Cytox assay kit (Daeil Lab Service, Seoul, Korea).

Park Y.S., Lee J.E., Park J.I., Myung C.H., Lim Y.H., Park C.K, & Hwang J.S. (2018). Inhibitory mechanism of ginsenoside Rh3 on granulocyte–macrophage colony-stimulating factor expression in UV-B–irradiated murine SP-1 keratinocytes. Journal of Ginseng Research, 44(2), 274-281.

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Viability Assay of Cultured Cells

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Crypts isolated from the aforementioned organs were seeded into 96 multiwell plates and cultured. After 4 days of incubation, cell viability was assayed with a EZ-Cytox assay kit (Daeil Lab Service, Seoul, Republic of Korea) by adding water-soluble tetrazolium salt to each well followed by 3 more hours of incubation. After the 3-hour incubation, only the medium was transferred to new wells in another 96-well plate, and the absorbance was measured at a wavelength of 450 nm with a VersaMax ELISA Microplate Reader (Molecular Devices, CA, USA).

Jee J.H., Lee D.H., Ko J., Hahn S., Jeong S.Y., Kim H.K., Park E., Choi S.Y., Jeong S., Lee J.W., Cho H.J., Kwon M.S, & Yoo J. (2019). Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture. Stem Cells International, 2019, 8472712.

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Assessing MCF-7 Cell Viability with SSF2 Compounds

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The viability of MCF-7 cells was assessed following treatment with SSF2-1, SSF2-2, and SSF2-3 using an Ez-CyTox assay kit (Daeil Lab Service Co., Seoul, Korea), as described in the literature [18 (link)]. Briefly, the MCF-7 cells were seeded onto 96-well plates and incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO₂. The cells were subsequently treated with SSF2-1, SSF2-2, and SSF2-3 and incubated for the indicated durations from 0 to 24 h at 37 °C in a humidified atmosphere containing 5% CO₂. For quantifying the cell viability, the cells that had been incubated for the desired durations were incubated with EZ-CyTox reagents for an additional 30 min at 37 °C in a humidified atmosphere containing 5% CO₂, and the optical density of each well was determined by measuring the absorbance at 450 nm using a microplate reader (Molecular Device, Palo Alto, CA, USA).

Lee D., Shim S, & Kang K. (2021). 4,6′-Anhydrooxysporidinone from Fusarium lateritium SSF2 Induces Autophagic and Apoptosis Cell Death in MCF-7 Breast Cancer Cells. Biomolecules, 11(6), 869.

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Cell Viability Assay with EZ-Cytox

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The cell viability/proliferation assay was performed with the EZ-Cytox assay kit (Daeil Lab Service, Seoul, Korea). The absorbance of the samples was measured using a microplate reader at 450 nm. The experiments were performed in triplicate.

Choi J.H., Kim Y.B., Ahn J.M., Kim M.J., Bae W.J., Han S.U., Woo H.G, & Lee D. (2018). Identification of genomic aberrations associated with lymph node metastasis in diffuse-type gastric cancer. Experimental & Molecular Medicine, 50(4), 6.

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Evaluating Combinatorial Therapies for 2D and 3D Cell Cultures

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After EL4 cells were cultured in 2D and in 3D for 10 days, cells were treated in serum-free media with doxorubicin (1 μM) alone or in combination with pretreatment of Tiam1/Rac1 inhibitor (100 μM NSC23766), Notch inhibitor (5 μM DAPT), or both for 24 h. To determine cell viability, the colorimetric WST-1 conversion assay (EZ-Cytox Assay Kit, Daeil Lab Service) was used. In brief, WST-1 reagent (20 μl) was added to each well, after which cells were incubated for 2 h in a humidified incubator at 37°C under 5% CO₂. The absorbance of the formazan dye, generated by the reaction between dehydrogenase and WST-1 in metabolically active cells, was measured using a microplate reader (Tecan, Männedorf, Switzerland) at 450 nm according to the manufacturer's instructions. Percent cell viability was calculated. The morphology and size of cell spheroids were assessed at desired time points under a phase contrast microscope. All experiments were performed at least thrice.

Ikram M., Lim Y., Baek S.Y., Jin S., Jeong Y.H., Kwak J.Y, & Yoon S. (2017). Co-targeting of Tiam1/Rac1 and Notch ameliorates chemoresistance against doxorubicin in a biomimetic 3D lymphoma model. Oncotarget, 9(2), 2058-2075.

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