NMR measurements were carried out according to a previously established protocol [14 (link)]. A Bruker Avance DRx 600 600-MHz spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany) was used to acquire all the spectra at 600.13 MHz. For all the samples, a spin–spin relaxation delay of 64 ms was used, and water-suppressed irradiation was applied during the relaxation delay (2 s). In the standard 1D and Carr–Purcell–Meiboom–Gill experiments, 256 transients were collected into 32 k data points, and the spectral width was 20 ppm.
Avance drx 600 600 mhz spectrometer
Manufactured by Bruker
Sourced in Germany, Japan, United States
The Avance DRx 600 is a 600-MHz NMR spectrometer manufactured by Bruker. It is designed to perform high-resolution nuclear magnetic resonance spectroscopy.
Automatically generated - may contain errors
3 protocols using avance drx 600 600 mhz spectrometer
1
NMR Spectroscopy Protocol for Biomolecules
2
Synthesis and Characterization of Fluorescent Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Starting materials were purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan), Nacalai Tesque Inc. (Kyoto, Japan), Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan), Sigma-Aldrich Co. (Tokyo, Japan), and were used without further purification unless otherwise stated. The spectroscopic data of 1a ,b were reported in the precedent reports [15 (link),17 (link)]. NMR spectra used in the characterization of products 2a –c were recorded on a JEOL ECA-600 600 MHz spectrometer (JEOL Ltd., Tokyo, Japan) and a Bruker AVANCE DRX-600 600 MHz spectrometer (Bruker, Massachusetts, USA). All NMR spectra were referenced to the solvent. UV-visible absorption spectra were recorded on a Hitachi U-3500 spectrometer (Hitachi High-Tech Science Corp., Tokyo, Japan). Fluorescence spectra and quantum yields were recorded on a Hitachi F-4500 fluorescence spectrometer (Hitachi High-Tech Science Corp., Tokyo, Japan) and a Hamamatsu Quantum Yields Measurements System for Organic LED Materials C9920-02 (Hamamatsu Photonics K.K., Hamamatsu, Japan), respectively. Matrix-assisted laser desorption ionization time-of-flight mass spectrometries (MALDI-TOF-MS) were recorded on a Shimadzu Axima-CFRplus (Shimadzu Corp., Kyoto, Japan). TLC analyses were carried out on aluminum sheets coated with silica gel 60 (Merck 5554). Column chromatography was performed on Wakogel C-300.
3
Stereochemistry of Whisky Lactones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Separation of the diastereoisomeric mixture of cis/trans-whisky lactones and chemical reduction of whisky lactones to corresponding diols were controlled by thin layer chromatography (TLC), using aluminum fail plates coated with silica gel. Compounds were detected by spraying the plates with 1% Ce(SO 4 ) 2 and 2% H 3 [P(Mo 3 O 10 ) 4 ] in 10% H 2 SO 4 . Gas chromatography analysis (GC, FID, carrier gas H 2 ) was carried out on Agilent Technologies 7890N (GC System, Santa Clara, CA, USA). Enantiomeric excesses of the products were determined on chiral column Cyclosil-B (30 m × 0.25 mm × 0.25 µm, Santa Clara, CA, USA) according to the next temperature program: 80 C/min) (1 min). Samples (2 µL) were injected with split 9:1; the flow of carrying gas was 1 mL/min. The total run time was 31.0 min. Retention times were established as follow: t R = 18.553 for anti-3-methyl-octane-1,4-diol (1a), t R = 18.630 min for syn-3-methyloctane-1,4-diol (1b). The structures of the compounds were confirmed on the basis of 1 H NMR and 13 C NMR, which were recorded for CDCl 3 solutions on a Bruker Avance DRX 600 (600 MHz) spectrometer (Billerica, MA, USA). IR spectra were determined using FTIR Thermo-Mattson IR 300 Spectrometer. Optical rotations were measured on a Jasco P-2000 Polarimeter.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!