Superoxide dismutase (SOD), lysozyme, and myeloperoxidase (MPO) activities in serum were assessed using an SOD activity colorimetric assay kit (BioVision), a lysozyme detection kit (Sigma-Aldrich), and an MPO colorimetric assay kit (Sigma-Aldrich), respectively, according to the manufacturers’ instructions.
Lysozyme detection kit
Manufactured by Merck Group
Sourced in United States
The Lysozyme detection kit is a laboratory tool designed to detect the presence and quantify the levels of the enzyme lysozyme. Lysozyme is an important biomolecule found in various biological samples, and its measurement is crucial in various applications. The kit provides a reliable and efficient method for determining lysozyme concentrations.
Automatically generated - may contain errors
4 protocols using lysozyme detection kit
1
Serum Antioxidant Enzyme Profiling
2
Quantifying Lysozyme Activity via Spectrophotometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lysozyme activity was measured using a lysozyme detection kit (Sigma-Aldrich, Cat. no. LY0100) according to the manufacturer’s instructions. The results of the lysozyme activity were defined by the lysis of Micrococcus lysodeikticus cells. The reactions were conducted at 25 °C, and the absorbance at 450 nm was measured on an ultraviolet-visible spectrophotometer (Perkin Elmer, Lambda XLS, Waltham, MA, USA).
df = dilution factor; 0.001 = ΔA450 as per unit definition; 0.03 = volume (in mL) of enzyme solution [31 (link)].
df = dilution factor; 0.001 = ΔA450 as per unit definition; 0.03 = volume (in mL) of enzyme solution [31 (link)].
3
Lysozyme Activity Assay Using Micrococcus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Activity of lysozyme was assayed by the lysozyme detection kit (Sigma-Aldrich, St. Louis, MO, USA) which uses Micrococcus lysodeikticus cell suspension as substrate39 (link).
4
Serum Biomarker Analysis in Immunology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum superoxide dismutase (SOD), lysozyme, and myeloperoxidase (MPO) activity was analyzed using the SOD activity colorimetric assay kit (BioVision), the lysozyme detection kit (Sigma–Aldrich), and the MPO colorimetric assay kit (Sigma–Aldrich), respectively, according to the manufacturers’ instructions. Respiratory burst (RB) was analyzed using nitro blue tetrazolium (NBT) assay, as previously described (Hasan et al., 2018 (link)). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total glucose, and total cholesterols levels were measured using Mindray commercial kits and a Mindray BS-390 automatic biochemistry analyzer (Mindray Bio-Medical Electronics, China) at the Core-Facility Center, Dong-eui University (Busan, South Korea).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!