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Sendai virus cantrell strain

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Sendai virus (Cantrell strain) is a laboratory-adapted strain of the Sendai virus. The Sendai virus is a member of the Paramyxoviridae family and is commonly used as a tool in biological research. The Cantrell strain has been widely utilized in various experimental applications.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (2 mentions) Dimethyl itaconate, by Merck Group (1 mentions) Sulforaphane, by Merck Group (1 mentions) Fetal calf serum (fcs), by R&D Systems (1 mentions) Lps from e coli 0111 b4, by InvivoGen (1 mentions) 4 octyl itaconate, by Merck Group (1 mentions) Thp 1 cell, by American Type Culture Collection (1 mentions) Phorbol 12 myristate acetate pma, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Nigericin, by InvivoGen (1 mentions) Adenosine triphosphate (atp), by InvivoGen (1 mentions) Pmscv pig, by Addgene (1 mentions) Pmscvneo, by Takara Bio (1 mentions) Polyinosinic polycytidylic acid poly 1 c, by InvivoGen (1 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Gm12878, by Coriell Institute for Medical Research (1 mentions) Genejuice, by Merck Group (1 mentions) Recombinant human m csf, by Thermo Fisher Scientific (1 mentions) Tofacitinib, by Merck Group (1 mentions)

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Sendai virus (13 citations)

4 protocols using sendai virus cantrell strain

1

Modulation of THP-1 Macrophages by Stimuli

Cited 2 times
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THP-1 cells (ATCC) were cultured in RPMI 1640 (Thermo) supplemented with 10% FCS (R&D) and 1% Pen/Strep (Thermo). LUCAT1−/− THP-1 have been generated previously (10 (link)). Cells were differentiated into macrophage-like cells using 10 ng/mL phorbol-12-myristate acetate (PMA, Sigma) for 24 h, followed by media change and resting in PMA-free medium for another 24 h before stimulation. Cells were treated with 200 ng/mL LPS from E. coli 0111:B4 (Invivogen), Sendai virus Cantrell strain (Charles River Laboratories), 10 ng/mL IFN-α 2b (Gemini), 100 µM to 250 µM 4-octyl itaconate, 250 µM di-methyl itaconate, 5 µM sulforaphane (all Sigma), or 500 nM diABZI-4 (GSK) (19 (link)).
Vierbuchen T., Agarwal S., Johnson J.L., Galia L., Lei X., Stein K., Olagnier D., Gaede K.I., Herzmann C., Holm C.K., Heine H., Pai A., O’Hara Hall A., Hoebe K, & Fitzgerald K.A. (2022). The lncRNA LUCAT1 is elevated in inflammatory disease and restrains inflammation by regulating the splicing and stability of NR4A2. Proceedings of the National Academy of Sciences of the United States of America, 120(1), e2213715120.
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2

Innate Immune Stimuli Protocols

Cited 1 time
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Lipopolysaccharide (LPS) and poly-dAdT (pdAdT) were obtained from Sigma-Aldrich and Immunostimulatory DNA oligonucleotides were synthesized as described (8 (link)). Nigericin and ATP were from Invivogen and Sigma respectively. Polyinosinic-polycytidylic acid (poly I:C) was obtained from Invivogen. Sendai virus (Cantrell strain) was purchased from Charles River Laboratories (Wilmington, MA). Lipofectamine 2000® Transfection Reagent was from Invitrogen. GeneJuice was from Novagen (Madison, WI). Universal type I IFN and IFN-γ were from PBL Interferon Source (Piscataway, NJ) and PeproTech (315-05), respectively. S. typhimurium (SL1344 lab strain) was from M. O’Riordan. The plasmids used were p65-pCMV4, c/EBPβ-pcDNA (Addgene), pGL3-enhancer luciferase reporter (Promega). Other plasmids such as Asc in pMSCVneo (Clontech), p205-HA in pRZ-retro, Aim2-FLAG, p204-HA, p205-HA in pEF-BOS or HA-tagged ΔHIN-p205, ΔPYD-p205 and ΔH/ΔP-p205 in pMSCV-PIG (Addgene) were made in the lab.
Ghosh S., Wallareth C., Covarrubias S., Hornung V., Carpenter S.B, & Fitzgerald K.A. (2017). The PYHIN protein p205 regulates the inflammasome by controlling Asc expression. Journal of immunology (Baltimore, Md. : 1950), 199(9), 3249-3260.
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3

Inducing Anti-Viral Immune Response in B-Lymphoblasts

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B-Lymphoblasts, GM12878, were obtained from Coriell Institute for Medical Research and cultivated according to the supplier's instructions. Fifteen percent fetal bovine serum was added to Roswell Park Memorial Institute media 1640 (RPMI-1640) with 2 mM L-glutamine for the culture. Sendai Virus (Cantrell strain) obtained from Charles River was used for inducing anti-viral immune response—50 µL of viral stock was added to 1 mL media. Cell samples were taken at 30 min, 1, 2, 4, 6, 12, 18, 24, 48, and 72 h after virus infection for the GRO-seq experiment. For the other experiments, 3C assay and the ChIP-seq experiment, the cells incubated for 6, 12, 18, and 24 h after infection were sampled. Untreated GM12878 cells were used as a control, which is the 0h sample.
Kim Y.J., Xie P., Cao L., Zhang M.Q, & Kim T.H. (2018). Global transcriptional activity dynamics reveal functional enhancer RNAs. Genome Research, 28(12), 1799-1811.
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4

Macrophage Activation and NF-κB Inhibition

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Reagents used in the study were obtained from following sources: E. coli LPS was purchased from Sigma-Aldrich (Cat#L2630); recombinant human M-CSF (Peprotech, #300-25), NF-κB inhibitor BAY-7082 (Tocris Bioscience, Cat#1744), Tofacitinib (Sigma, Cat#PZ0017), and HSV-1 (David Knipe Laboratory) Sendai virus (Cantrell strain) were purchased from Charles River Laboratories (Wilmington, MA), GeneJuice was from Millipore, #70967-6. Customized nCounter gene expression code-set was obtained from NanoString technologies (Seattle, WA).
Agarwal S., Vierbuchen T., Ghosh S., Chan J., Jiang Z., Kandasamy R.K., Ricci E, & Fitzgerald K.A. (2020). The long non-coding RNA LUCAT1 is a negative feedback regulator of interferon responses in humans. Nature Communications, 11, 6348.
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