Ft raman module

FT Raman spectra of selected fractions (range 100–4000 cm⁻¹, 1000 scans, resolution 4 cm⁻¹) were recorded on Nicolet iS50 with the FT Raman module (ThermoFisher Scientific, Waltham, MA, USA), Nd:YAG laser (λ_ex = 1064 nm, power 500 mW), CaF₂ beam splitter and InGaAs detector. Vibration spectra were recorded and processed using Omnic 9.0 software (ThermoFisher Scientific, Waltham, MA, USA). The raw spectra were exported in ASCII format to Origin 6.0 software (OriginLab, Northampton, MA, USA) for further processing (smoothing, baseline correction), calculation of the average spectra.

FT-MIR ATR spectra (range 650–4000 cm⁻¹, resolution 2 cm⁻¹, 64 scans) were recorded on FT-IR spectrometer Nicolet 6700 (Thermo Fisher Scientific, Waltham, MA, USA) using KBr beam splitter and smart MIRacle holder. FT-NIR DR spectra (range 10,000–4000 cm⁻¹, resolution 8 cm⁻¹, 64 scans) were recorded on the same spectrometer using CaF₂ beam splitter and a smart NIR UpDRIFT holder. FT-Raman spectra (range 100–4000 cm⁻¹, 1000 scans, resolution 4 cm⁻¹) were recorded with FT-Raman spectra were measured with Nicolet iS50 with FT Raman module (Thermo Fisher Scientific, Waltham, MA, USA), Nd:YAG laser (λ_ex = 1064 nm, power 500 mW), CaF₂ beam splitter and InGaAs detector. Vibration spectra were recorded and processed using software Omnic 8.0 and 9.0 (Thermo Fisher Scientific, Waltham, MA, USA). The raw spectra were exported in ASCII format to Origin 6.0 software (Microcal Origin, Northampton, MA, USA) for further processing (smoothing, baseline correction), calculation of the average spectra of each sample recorded for 100 (FT-MIR, FT-NIR) or 10 (FT-Raman) randomly chosen pollen loads and preparation of the graphs.