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Immunobiolon p membrane

Manufactured by Merck Group
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Sourced in Germany

The Immunobiolon-P membrane is a specialized laboratory equipment designed for filtration and separation applications. It is a microporous membrane made of polyvinylidene fluoride (PVDF) material, which is known for its excellent chemical and thermal resistance properties. The Immunobiolon-P membrane is primarily used for the filtration and purification of proteins, peptides, and other biomolecules in various research and development settings.

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Lab products found in correlation

Cell extraction buffer, by Thermo Fisher Scientific (3 mentions) Supersignal west pico chemiluminescent substrated, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PES membrane (18 citations)

3 protocols using immunobiolon p membrane

1

Western Blot Analysis of IEC Innate Immune Signaling

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Total cell lysates of IECs transfected with Poly I:C was prepared by using the cell extraction buffer (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions. Equal amounts of protein lysates (30 µg) were separated on 4–12% sodium dodecyl sulfate polyacrylamide gel electrophoresis precast gels and transfected to an Immunobiolon-P membrane (Millipore, Eschborn, Germany). The blots were incubated with primary antibodies in 5% nonfat milk in PBS with 0.05% Tween 20 (PBST) overnight at 4°C (IRF3, 1:1,000; Phospho-IRF3, 1:1,000; IRF7, 1:1,000; Phospho-IRF7, 1:1,000; GAPDH, 1:5,000; β-actin, 1:5,000; EEA1, 1:1,000; CD63, 1:1,000; LAMP2, 1:2,000; Alix, 1:1,000; ISG15, 1:1,000; ISG56, 1:1,000; GBP5, 1:1,000; Viperin, 1:1,000; MxA, 1:1,000; MxB, 1:1,000; OAS-1, 1:1,000). All antibodies were obtained from Cell Signaling Technology (Cell Signaling Technology, MA, USA) Horseradish peroxidase-conjugated appropriate second antibodies were diluted at 1:2,000 to 1:8,000 in 2% nonfat milk PBST. Blots were developed with SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, MA, USA).
Guo L., Xu X.Q., Zhou L., Zhou R.H., Wang X., Li J.L., Liu J.B., Liu H., Zhang B, & Ho W.Z. (2018). Human Intestinal Epithelial Cells Release Antiviral Factors That Inhibit HIV Infection of Macrophages. Frontiers in Immunology, 9, 247.
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2

Western Blot Protein Analysis

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Total cell lysates were prepared by the cell extraction buffer (Invitrogen, Shanghai, China) with 1% protease inhibitor cocktail (Sigma, MO) according to the manufacturer’s instructions. Equal amounts of protein lysates were separated on 4–12% sodium dodecyl sulfate polyacrylamide gel electrophoresis precast gels an d transferred to an Immunobiolon-P membrane (Millipore, Germany). Nonspecific sites were blocked with 5% nonfat dried milk before being incubated with antibody. Blots were developed with SuperSignal West Pico Chemiluminescent Substrated (Thermo Fisher Scientific, Waltham). Densitometric analysis was performed by using ImageJ 1.44 software (National Institutes of Health).
Xu X.Q., Liu Y., Zhang B., Liu H., Shao D.D., Liu J.B., Wang X., Zhou L.N., Hu W.H, & Ho W.Z. (2019). IL-22 Suppresses HSV-2 Replication in Human Cervical Epithelial Cells. Cytokine, 123, 154776.
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3

Protein Quantification and Western Blotting

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Total cell lysates were prepared by the cell extraction buffer (Invitrogen, Shanghai, China) with 1% protease inhibitor cocktail (Sigma, MO, USA) according to the manufacturer’s instructions. Equal amounts of protein lysates were separated on 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis precast gels and transferred to an Immunobiolon-P membrane (Millipore, Germany). Nonspecific sites were blocked with 5% nonfat dried milk before being incubated with Antibody. Blots were developed with SuperSignal West Pico Chemiluminescent Substrated (Thermo Fisher Scientific, Waltham, MA, USA). Densitometric analysis was performed by using ImageJ 1.44 software (National Institutes of Health).
Xu X.Q., Guo L., Wang X., Liu Y., Liu H., Zhou R.H., Gu J., Liu J.B., Xu P., Zhou L, & Ho W.Z. (2018). Human Cervical Epithelial Cells Release Antiviral Factors and Inhibit HIV Replication in Macrophages. Journal of Innate Immunity, 11(1), 29-40.
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