Log In Sign up
The largest database of trusted experimental protocols

Cd3 percp cy5

Manufactured by Beckman Coulter
Visit Supplier

CD3 PerCP-Cy5.5 is a fluorescent-conjugated antibody designed for flow cytometry analysis. It specifically binds to the CD3 antigen, which is a component of the T cell receptor complex expressed on the surface of T lymphocytes. This product is intended for research use only.

Automatically generated - may contain errors

Lab products found in correlation

Cd4 fitc, by Beckman Coulter (2 mentions) Cd56 pe, by Beckman Coulter (2 mentions) Cd8 fitc, by Beckman Coulter (1 mentions) Cd25 pe, by Beckman Coulter (1 mentions) Lag 3 pe, by BioLegend (1 mentions) Cd137 pe, by BioLegend (1 mentions) Tim 3 pe cy7, by BioLegend (1 mentions) Cxp analysis software, by Beckman Coulter (1 mentions) Cd28 pe, by Beckman Coulter (1 mentions) Cytomics fc500 flow cytometer, by Beckman Coulter (1 mentions) Cd69 ecd, by Beckman Coulter (1 mentions) Qdot 655, by Thermo Fisher Scientific (1 mentions) Cd4 percp cy5, by Beckman Coulter (1 mentions) Ccr7 fitc, by R&D Systems (1 mentions) Mip 1β pe, by Beckman Coulter (1 mentions) Ccr7 percp cy5, by Thermo Fisher Scientific (1 mentions) Cd3 pe cy7, by Beckman Coulter (1 mentions) Cd95 apc, by Thermo Fisher Scientific (1 mentions) Cd28 pe cy7, by Thermo Fisher Scientific (1 mentions) Perforin fitc, by Mabtech (1 mentions)

3 protocols using cd3 percp cy5

1

Phenotypic Characterization of Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Various subpopulations within the cell products (PBMCs prior to culture and cultured DC-CIKs) were identified by flow cytometry, as previously described (11 (link)), using the following fluorochrome-conjugated antibodies: CD3 PerCP-Cy5.5, CD4 FITC, CD8 FITC, CD25 PE, CD28 PE, CD56 PE (all Beckman Coulter, Inc.), PD-1 PE, lymphocyte-activation gene 3 (LAG-3) PE, tumor necrosis factor receptor superfamily member 9 (4-1BB; CD137) PE and T cell immunoglobulin and mucin protein 3 (TIM-3) PeCy-7 (all BioLegend, Inc.). Three-color flow cytometry analysis was performed on a Cytomics FC500 flow cytometer with CXP analysis software (Beckman Coulter, Inc.).
Huang L., Qiao G., Morse M.A., Wang X., Zhou X., Wu J., Hobeika A., Ren J, & Lyerly H.K. (2019). Predictive significance of T cell subset changes during ex vivo generation of adoptive cellular therapy products for the treatment of advanced non-small cell lung cancer. Oncology Letters, 18(6), 5717-5724.
+ Open protocol
+ Expand
2

Multiparameter Flow Cytometry Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Conjugated antibodies and staining reagents included MIP-1β-PE, CD3-PB, CD3-PE-Cy7, CD3-PerCP-Cy5.5, CD3-APC-Cy7, CD3-Horizon V450, CD4-PerCP-Cy5.5, CD4-AmCyan, CD4-FITC, CD8α-APC, CD8α-APC-Cy7, CD8α-AlexaFluor700, CD8α-FITC, CD8α-APC-H7, CD69- ECD (Beckman Coulter), CD20-Horizon V450, CCR7-FITC (R&D Systems), CD95-APC, CCR7-PerCP-Cy5.5, CD28-PE-Cy7 (eBioscience), granzyme B-AlexaFluor700, perforin-FITC (MabTech), IFNγ-PE-Cy7, TNFα- AlexaFluor700, IL-2-APC, CD95-PE, CD95-APC, and Aqua LIVE/DEAD Fixable Dead Cell Stain (Invitrogen). All reagents are from BD Biosciences unless indicated otherwise. For construction of monomers and tetramers, the following peptides were synthesized and purified to >95% by HPLC by New England Peptide LLC: p11C (CTPYDINQM), p54AS (TVPWPNASL), p54E660 (TVPWPNETL), p68A (STPPLVRLV), and TL8 (TTPESANL). The monomers and tetramers were prepared as previously described [85] (link), [86] (link). Tetramers were prepared using either streptavidin-PE (Prozyme), -APC (Prozyme), -AlexaFluor488 (Invitrogen), or -Qdot655 (Invitrogen). Monomers used in surface plasmon resonance studies were further quantified using an RC DC protein kit (Bio-Rad).
Osuna C.E., Gonzalez A.M., Chang H.H., Hung A.S., Ehlinger E., Anasti K., Alam S.M, & Letvin N.L. (2014). TCR Affinity Associated with Functional Differences between Dominant and Subdominant SIV Epitope-Specific CD8+ T Cells in Mamu-A*01 + Rhesus Monkeys. PLoS Pathogens, 10(4), e1004069.
+ Open protocol
+ Expand
3

Immune Cell Profiling in Leukapheresis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The quantity and percentage of various immune cell types in leukapheresis product and elutriation fractions were analyzed by an ABX Pentra 60 hematology analyzer (HORIBA). For other experiments, FACS analysis (Accuri C6 Plus; Beckman Coulter Life Sciences) was used. Monocytes were enumerated by CD45 + /CD14 + and propidium iodideÀnegative population (Beckman Coulter Life Sciences). Dendritic cells were gated by forward scatter and side scatter on the basis of cell size and cellular complexity, and further gated by anti-CD45 and propidium iodide. Phenotypic analyses of dendritic cells were performed using anti-CD209-APC, CD80-PE, CD83-PE, CD86-PE, CD11c-PE, HLA-DR-APC, HLA-ABC-APC and CCR7-APC (Beckman Coulter Life Sciences). To analyze cell type compositions in CUD-002 product, anti-CD45-APC, CD19-PE, CD20-PE, CD3-Percp-Cy5.5, CD56-PE, CD34-PE, lineage cocktail (CD3/CD14/CD16/CD19/CD20/CD56)-FITC, CD13-PE, CD33-PE and S100A9-PE (Beckman Coulter Life Sciences) were used.
Li Q., Yang C., Tian H., Jiang J., Li P., Zhu X., Lei T., Yin R., Ding P., Bai P, & Li Q. (2023). Development of a personalized dendritic cell vaccine and single-cell RNA sequencing-guided assessment of its cell type composition. Cytotherapy, 25(2).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!