Six-week old female BALB/c mice were purchased from Shanghai Laboratory Animal center of the Chinese Academy of Sciences and housed under aseptic and ventilated condition. The mice were inoculated by subcutaneous injection with MCF-7 cancer cells suspended in 5% saline. Tumor volume and weight were measured on alternate days. Body weights were monitored as an indicator of toxicity. When tumor volume exceeds100 mm3, mice were randomly divided into treatment and control groups (n=8 per group): QDAU5 and vehicle control (0.5% CMC-Na). Treatment groups were administered orally with QDAU5 daily at doses of 20, 40, and 80 mg/kg, respectively. Treatment started from the next day and continued for 21 days. All mice were killed at the end of the experiment, and subcutaneous tumors were removed and weighted.
Balb c mice
Manufactured by Shanghai Laboratory Animal Center
Sourced in China
BALB/c mice are an inbred strain of laboratory mice commonly used in biomedical research. They are a versatile and well-characterized model organism with a stable genetic background.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop 2000, by Thermo Fisher Scientific (4 mentions)
C57bl 6, by Shanghai Laboratory Animal Center (3 mentions)
Balb c nude mice, by Shanghai Laboratory Animal Center (3 mentions)
C57bl 6j, by Shanghai Laboratory Animal Center (2 mentions)
Quick antibody adjuvant, by Suzhou Biodragon Immunotechnologies Co. (2 mentions)
Mouse monoclonal antibody isotyping kit, by Bio-Rad (2 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Hep 2 cells, by American Type Culture Collection (1 mentions)
Isoflurane, by Baxter (1 mentions)
Aerrane, by Baxter (1 mentions)
Aluminum hydroxide, by Thermo Fisher Scientific (1 mentions)
Wright giemsa staining kit, by Nanjing Jiancheng (1 mentions)
Dba 1j, by Shanghai Laboratory Animal Center (1 mentions)
N3876, by Merck Group (1 mentions)
Ly294002, by Selleck Chemicals (1 mentions)
Alphalmager 2000 imaging system, by Bio-Techne (1 mentions)
Streptomycin, by BD (1 mentions)
Penicillin, by BD (1 mentions)
Sprague dawley rats, by Shanghai Laboratory Animal Center (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
51 protocols using balb c mice
1
Antitumor Effects of QDAU5 in BALB/c Mice
2
Acute Lung Injury Model and Antagomir-598 Treatment
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BALB/c mice at 5–6 weeks old were purchased from Shanghai Laboratory Animal Center (Shanghai, China). Randomly grouped mice received 1.4% isoflurane in 100% oxygen, and were intratracheally administrated with 5 mg/kg of LPS (Sigma–Aldrich, USA) to establish the acute lung injury model. For the treatment group, mice (n = 5) were injected with antagomir-598 (30 mg/kg/day) or antagomir control via the tail vein for a consecutive 3 days. The efficiency was analyzed 1 week later in murine lungs. All animal experiment were approved by the Animal Care and Use Committee of Shaanxi University of Chinese Medicine.
3
Mouse Strain Maintenance and Usage
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C57BL/6J and BALB/c mice were purchased from the Shanghai Experimental Animal Center (Shanghai, China). Rag2–/– mice were provided by Dr. X. Wang (Inner Mongolia University). All mice were maintained in a specific pathogen free facility and used according to the guidelines for experimental animals at the University of Science and Technology of China. Mice were used between 6 weeks and 8 weeks of age.
4
In vivo Evaluation of ABT-199 Efficacy
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To test the in vivo efficiency of ABT-199, BALB/c mice (5–6-week-old, obtained from Shanghai Laboratory Animal Center, Shanghai, China) were injected with 1 × 107 A20 cells into the right flank. Treatments started after tumor became about 0.5 cm × 0.5 cm in surface (Day 0). For oral treatment, ABT-199 (10 mg/ml) was formulated in 60% phosal 50 propylene glycol, 30% polyethyleneglycol-400 and 10% ethanol. Tumor volumes were calculated as 0.5 × a(length) × b(width) 2.
5
BALB/c Murine Model of RSV Infection
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Six-week-old female BALB/c mice (Shanghai Laboratory Animal Center, Shanghai, China) were bred and maintained in a specific-pathogen-free facility at the Laboratory Animal Center, China Medical University. All experiments were implemented in accordance with the guidelines and regulations approved by the Institutional Animal Care and Use Committee of China Medical University, China. The human RSV type A2 (RSV A2) strain was propagated in HEP-2 cells (ATCC). Virus titer was expressed as a 50% tissue culture infection dose (TCID50), calculated using the method of Reed and Muench [22 (link)]. For RSV inoculation, mice were inoculated intranasally at an inoculum dose of 2 × 106 TCID50 per mouse in 20 μL of sterile phosphate-buffered saline (PBS). As controls, mice were inoculated intranasally with sterile PBS.
6
Tumor Growth Monitoring in Mice
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Animal care protocols and experiments were approved by the animal ethical committee of Luoyang Orthopedic Hospital of Henan Province and the Orthopedic Hospital of Henan Province and performed in accordance with the guidelines specified in the Guide for the Care and Use of Laboratory Animals (Ministry of Science and Technology of China, 2006). Twenty female BALB/c mice (4–6 weeks old) were purchased from the Shanghai Laboratory Animal Center (Shanghai, China). Cells (1 × 106) were subcutaneously injected into each mouse. Nude mice were anesthetized with a 3–5% (v/v) mixture of isoflurane (Aerrane; isoflurane, Baxter) in synthetic air (200 ml/min), killed by cervical dislocation, and the tumors were obtained. The body weight and tumor length and width were measured in each mouse every 3 days. Additionally, tumor volume was calculated using the formula: volume = 1/2 (width2 × length). After 4 weeks, the mice were euthanized, and the tumors excised and weighed.
7
4T1 Breast Cancer Mouse Model
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All animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of Zhejiang Chinese Medical University and experiments were approved by the Animal Ethics Committee of Zhejiang Chinese Medical University. 6 week female Balb/c mice, with an average weight of 17 g, were provided by the Shanghai Laboratory Animal Center, Shanghai, China. Mice were injected with 1 × 106 4T1 cells (0.1 mL cells in PBS) subcutaneously at the right axillary region.
8
Culturing CT26 Cells in Balb/c Mice
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The CT26 colorectal tumor cells were obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China), and incubated in RPMI 1640 medium with 10% fetal bovine serum and 1% penicillin‐streptomycin at 37 °C in 5% CO2 incubator. Four‐week‐old female Balb/c mice were obtained from the Shanghai Experimental Animal Center (Shanghai, China).
9
Murine Breast Tumor Cell Culture
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4T1 murine breast tumor cell was obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China). The cells were cultured in complete RPMI 1640 cell culture medium containing 10% fetal bovine serum (FBS), 2.5 g/L of glucose, 0.11 g/L of sodium pyruvate, 100 U/mL of penicillin G sodium and 100 μg/mL of streptomycin sulfate. The cells were incubated at 37 °C with 5.0% CO2 atmosphere. Four-week-old BALB/c mice (18–20 g, female) were obtained from the Shanghai Experimental Animal Center (Shanghai, China). Animal procedures were carried out under the guidelines approved by the Institutional Animal Care and Use Committee (IACUC) of the Shanghai Institute of Material Medica, Chinese Academy of Sciences.
10
Murine Breast and Colorectal Tumor Cell Lines
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4T1 murine breast tumor cells and CT26 murine colorectal tumor cells were both supplied by the cell bank of Chinese Academy of Sciences (Shanghai, China). Both types of cells were maintained in high-glucose Dulbecco's modified Eagle medium (DMEM) supplemented with 10% (v/v) of fetal bovine serum (FBS) and 1% penicillin-streptomycin. Both types of cells were incubated at 37 °C under a humidified atmosphere containing 5% of CO2. All experiments were performed in the logarithmic phase of the cell growth.
BALB/c mice (female, 18–20 g, 4-week-old) were purchased from the Shanghai Experimental Animal Center (Shanghai, China). All animal procedures were conducted under the guidelines approved by the Institutional Animal Care and Use Committee (IACUC) of Shanghai Institute of Material Medica, Chinese Academy of Sciences (Shanghai, China).
BALB/c mice (female, 18–20 g, 4-week-old) were purchased from the Shanghai Experimental Animal Center (Shanghai, China). All animal procedures were conducted under the guidelines approved by the Institutional Animal Care and Use Committee (IACUC) of Shanghai Institute of Material Medica, Chinese Academy of Sciences (Shanghai, China).
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