Whatman 903 protein saver card

Manufactured by GE Healthcare

Sourced in United States, United Kingdom

Whatman 903 Protein Saver cards are laboratory equipment designed to collect and store biological samples, such as blood and other bodily fluids. These cards provide a reliable and convenient method for sample preservation and storage.

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Spelling variants of this product

Whatman 903 (36 citations) Whatman 903 cards (6 citations) Protein Saver 903 (3 citations) DBS cards (Whatman 903 Protein Saver) (2 citations) 903 Specimen Collection Paper/Whatman protein saver cards (2 citations)

39 protocols using whatman 903 protein saver card

Sickle Cell Disease Diagnostic Workflow

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Four milliliters of venous blood were collected into EDTA a vac-utainer. Samples were kept at room temperature (22°C–27°C) and tested using rapid sickle cell test within 4 hours.
The preparation of a DBS was made by dispensing 100 µL of blood to a well labeled Whatman™ 903 Protein Saver Card (GE Healthcare Ltd, Cardiff, UK), and stored in a humid free zip-locked bag with desiccant packets at −20°C until shipped to CPHL for analysis.

Mandu K., Tusuubira S.K., Mwambi B., Webbo F., Atuhairwe C, & Taremwa I.M. (2018). To test or not: occurrence of sickle cell trait and assessment of the awareness toward its screening among patients attending Magale Health Center IV, Namisindwa District, Eastern Uganda. Journal of Blood Medicine, 9, 219-225.

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Dried Blood Spot Toxoplasma and HIV Testing

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Blood spots were obtained as described in eMethods 2 in the Supplement. Blood spots (2 drops of approximately 80 μL per spot) were collected on Whatman 903 Protein saver card (GE Healthcare), passively dried at ambient room temperature (typically 25°C to 30°C), exported, and then stored at −80°C prior to analysis. Toxoplasma gondii immunoglobulin G (IgG) status (TOXO IgG enzyme-linked immunosorbent assay; DIAsource ImmunoAssays SA) and HIV-1 and HIV-2 status (Murex HIV-1.2.O; DiaSorin S.p.A.) were determined from the dried blood spot samples described in eMethods 3 in the Supplement.

Steptoe P.J., Momorie F., Fornah A.D., Komba P., Emsley E., Scott J.T., Williams S.J., Harding S.P., Vandy M.J., Sahr F., Beare N.A, & Semple M.G. (2020). Evolving Longitudinal Retinal Observations in a Cohort of Survivors of Ebola Virus Disease. JAMA Ophthalmology, 138(4), 395-403.

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Newborn Sickle Cell Screening Protocol

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For initial development of the paper-based newborn SCD screening test, blood samples were collected from SCD (HbSS) patients at the Texas Children’s Hematology Center and from healthy volunteers (HbAA) into Vacutainer vials (K₂EDTA, BD Diagnostics, USA) using standard venipuncture technique. Samples were stored at 4 °C until use. Artificially reconstituted samples with a range of HbS levels were created by mixing ABO/Rh-matched, equal-hematocrit HbAA and HbSS blood samples at various ratios.
For test validation in Cabinda, blood samples were collected from newborns by heel-stick onto blood collection cards (Whatman 903 Protein Saver Card, GE Healthcare, USA) and into capillary blood collection tubes (Microvette, K₂EDTA, Sarstedt AG & Co, Germany). Eluted dried blood spot samples were tested with isoelectric focusing (IEF) following existing standard operating procedures7 (link). Liquid blood samples were refrigerated and used to perform the paper-based test within 7 days of collection. For all patients, the paper-based test was completed before IEF analysis. Local health workers interpreted the results of the paper-based test visually, using reference images of HbS-positive and HbS-negative bloodstains.

Piety N.Z., George A., Serrano S., Lanzi M.R., Patel P.R., Noli M.P., Kahan S., Nirenberg D., Camanda J.F., Airewele G, & Shevkoplyas S.S. (2017). A Paper-Based Test for Screening Newborns for Sickle Cell Disease. Scientific Reports, 7, 45488.

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SARS-CoV-2 Antibody DBS Assessment

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A panel consisting of 10 unique SARS-CoV-2 antibody positive DBS cards were contrived and 10 unique SARS-CoV-2 negative DBS cards were directly spotted from EDTA whole blood to assess the performance of commercial and in-house serological tests (S4 Fig). Each testing site was blinded to the status of the DBS cards. SARS-CoV-2 antibody positive plasma samples were contrived into DBS specimens by using blood collected from healthy donors within the National Microbiology Laboratory. SARS-CoV-2 antibody negative blood was centrifuged at 1,500 RPM for 7 minutes and the plasma was removed. The remaining red blood cells were re-suspended with SARS-CoV-2 antibody positive plasma using a 1:1 ratio and 75 μL was spotted onto each circle of a Whatman 903 Protein Saver card (GE Healthcare, Boston, MA). Spotted cards were allowed to air-dry for at least 2 hours in a biosafety cabinet and then packaged in a gas impermeable bag with a desiccant pack and a humidity indicator card. Packaged cards (maximum 10 per bag) were stored at -80°C until further testing. SARS-CoV-2 antibody negative blood was spotted directly from the EDTA Vacutainer tubes onto Whatman 903 Proteinsaver cards as described above.

Cholette F., Mesa C., Harris A., Ellis H., Cachero K., Lacap P., Galipeau Y., Langlois M.A., Gingras A.C., Yansouni C.P., Papenburg J., Cheng M.P., Chakraborty P., Stein D.R., Van Caeseele P., Bartlett S., Krajden M., Goldfarb D., McGeer A., Osiowy C., Hankins C., Mazer B., Drebot M, & Kim J. (2021). Dried blood spot specimens for SARS-CoV-2 antibody testing: A multi-site, multi-assay comparison. PLoS ONE, 16(12), e0261003.

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Dried Blood Spot Sample Collection

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(2)

Whatman903 card (Whatman903 protein saver card, GE Healthcare Europe, Freiburg, Germany)

(3)

HemaSpot (HemaSpot-HF, Spot On Sciences, Austin, Texas)

Yamamoto C., Nagashima S., Isomura M., Ko K., Chuon C., Akita T., Katayama K., Woodring J., Hossain M.S., Takahashi K, & Tanaka J. (2020). Evaluation of the efficiency of dried blood spot-based measurement of hepatitis B and hepatitis C virus seromarkers. Scientific Reports, 10, 3857.

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Biosampling for Neonatal miRNA Profiling

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The biosamples chosen for determining miRNA expression levels were plasma, EDTA-blood, urine, and DBS collected at a median time of 18-19 h of age in both groups and after the infants in the cooled group had reached their target core temperature (33.5 °C). Of the 1.5 ml of blood obtained from the newborn, one drop was placed on absorbent filter paper (Whatman 903 protein saver card, GE Healthcare Life Sciences, Little Chalfont, UK) creating a DBS. This was allowed to dry at room temperature and was then stored in a polythene bag with a packet of desiccant to sustain desiccation. The remaining blood was transferred into tubes with sprayed-coated K2EDTA (Fisher Scientific, Loughborough, UK) and then centrifuged at 15,000g for 10 min to separate the plasma and blood components (EDTA-blood). The plasma, EDTA-blood, and urine collected from a bag or a catheter sample were all stored at -80 °C until further processed.

Ponnusamy V., Kapellou O., Yip E., Evanson J., Wong L.F., Michael-Titus A., Yip P.K, & Shah D.K. (2016). A study of microRNAs from dried blood spots in newborns after perinatal asphyxia: a simple and feasible biosampling method. Pediatric research, 79(5).

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Evaluation of PLD Inhibitor Effects

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The PLD inhibitors 5-fluoro-2-indolyl des-chlorohalopemide hydrochloride hydrate (FIPI), sodium metavanadate (NaVO₃), and sodium tungstate hydrate (Na₂WO₄) were obtained from Sigma–Aldrich Sweden AB (Stockholm, Sweden).
The DBS filter paper cards and devices for microsampling of blood examined were the Whatman 903 Protein Saver card (GE Healthcare Ltd., Cardiff, UK), the Capitainer quantitative DBS (qDBS; Capitainer AB, Stockholm, Sweden), the 10 μL Mitra Clamshell (Neoteryx, Torrance, CA, USA), and the HemaXis DB 10 (DBS System SA, Gland, Switzerland). The addition of PLD inhibitors to dismounted Capitainer qDBS discs was made by pipetting 2-μL inhibitor solution onto the disc and allowing it to dry for at least 24 h.

Beck O., Mellring M., Löwbeer C., Seferaj S, & Helander A. (2021). Measurement of the alcohol biomarker phosphatidylethanol (PEth) in dried blood spots and venous blood—importance of inhibition of post-sampling formation from ethanol. Analytical and Bioanalytical Chemistry, 413(22), 5601-5606.

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Cholera Diagnosis from Stool Samples

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Upon admission, a stool sample was collected in a clean (non-disinfected), unused container. Two drops of watery stool were transferred to a vial containing 3 ml of APW broth and kept at ambient temperature for 4 to 6 hours.
Study staff soaked two 6-mm filter paper disks in each fresh watery stool sample and then placed each filter paper into separate microtubes with two drops of normal saline. One microtube was sent immediately to the National Public Health Laboratory (NPHL) in Juba, and the other was stored at ambient temperature until the end of the study and sent to Institut Pasteur (IP), Paris, France.
Using a Pasteur pipette, one to two drops of direct stool or enriched APW medium were spotted onto a Whatman 903 Protein Saver Card (GE Healthcare Ltd., Forest Farm, Cardiff, UK) and allowed to air-dry. Dry filter papers were packed individually with a desiccant bag and stored at ambient temperature until processing. Dried filter papers were sent to Johns Hopkins University (JHU) to be tested for V. cholerae using molecular methods.

Ontweka L.N., Deng L.O., Rauzier J., Debes A.K., Tadesse F., Parker L.A., Wamala J.F., Bior B.K., Lasuba M., But A.B., Grandesso F., Jamet C., Cohuet S., Ciglenecki I., Serafini M., Sack D.A., Quilici M.L., Azman A.S., Luquero F.J, & Page A.L. (2016). Cholera Rapid Test with Enrichment Step Has Diagnostic Performance Equivalent to Culture. PLoS ONE, 11(12), e0168257.

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Dried Blood Spot Serum Immunogenicity Study

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We collected serum samples pre- (day 0) and post- (day 21 and 35) vaccination as a part of an OCV immunogenicity study in an internally displaced persons camp in Juba, South Sudan [23 (link)]. Blood (collected either via venipuncture or by finger prick from volunteers) was spotted on Whatman 903 protein saver cards (Whatman 903, GE Healthcare, LifeSciences). We collected a total of 5 blood spots per DBS card from each volunteer for each time point (S1 Fig). Samples reached approximately 50% saturation per spot. Each DBS card was put in a biohazard plastic bag with dessicator and stored at ambient temperature in sub-Saharan Africa. The median storage days for the samples used were 70, 47 and 34 days, for pre-vaccination (D0) and post-vaccination days 21 and 35, respectively. Samples were then shipped by postal service to the University of Utah where they were stored at -20°C for approximately 9 months or longer until analysis.

Iyer A.S., Azman A.S., Bouhenia M., Deng L.O., Anderson C.P., Graves M., Kováč P., Xu P., Ryan E.T., Harris J.B., Sack D.A., Luquero F.J, & Leung D.T. (2018). Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses. PLoS Neglected Tropical Diseases, 12(1), e0006196.

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Anti-Ov16 Antibody Sample Preparation

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Blood samples containing the anti-Ov16 positive control antibody were made by diluting the recombinant IgG4 from a stock solution into FBS (Invitrogen, Grand Island, NY) and then mixing thoroughly at a 1:1 dilution with packed, washed, red blood cells. 75μL per circle marking of the contrived whole blood sample was spotted on Whatman 903 Protein Saver Cards (GE Healthcare, Pittsburgh, PA). These cards were then dried overnight in ambient laboratory conditions, then stored at -20°C with approximately ten cards per sealed foil pouch containing two-unit clay desiccant packets (Desiccare, Reno, NV).

Golden A., Stevens E.J., Yokobe L., Faulx D., Kalnoky M., Peck R., Valdez M., Steel C., Karabou P., Banla M., Soboslay P.T., Adade K., Tekle A.H., Cama V.A., Fischer P.U., Nutman T.B., Unnasch T.R., de los Santos T, & Domingo G.J. (2016). A Recombinant Positive Control for Serology Diagnostic Tests Supporting Elimination of Onchocerca volvulus. PLoS Neglected Tropical Diseases, 10(1), e0004292.

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