Trifluoroacetic acid (TFA), Triisopropylsilane (TIS), Fmoc amino acids and coupling reagent O-(7-Azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU) and benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate (PyBop) were supplied by the Merck Co, Germany. Solvents, acetonitrile (MeCN), Piperazine, N, N-diisopropylethylamine (DIPEA), diethylether, Dichloromethane (DCM), N, N-dimethylformamide (DMF), and methanol (MeOH) were purchased from the Merck Co, Germany. 2-Chlorotritylchloride(2-CTC) resin (1% DVB, 200-400 mesh, 1 mmol/g) was from the Santa Cruz Biotechnology Co, USA. Commercially available chemicals were used as received unless otherwise stated. Fourier Transform Infrared spectra were obtained by the Shimadzu recording spectrometer, Japan. The mass spectral measurements were performed using a 6410 Agilent LCMS triple quadrupole mass spectrometer (LCMS) with an electrospray ionization (ESI) interface, USA. The cell lines were purchased from Iranian Biological Resource Center (IBRC), Tehran, Iran.
N dimethylformamide dmf
Manufactured by Merck Group
Sourced in United States, Germany, India
N-dimethylformamide (DMF) is a highly polar aprotic solvent commonly used in various laboratory applications. It has a high boiling point and is miscible with a wide range of organic solvents. DMF is a versatile chemical that can serve as a reaction medium, a solvent for extraction, and a reagent in organic synthesis.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Methanol, by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Acetone, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Triisopropylsilane, by Merck Group (1 mentions)
Piperazine, by Merck Group (1 mentions)
O 7 azabenzotriazol 1 yl n n n n tetramethyluronium hexafluorophosphate hatu, by Merck Group (1 mentions)
6410 agilent lc ms triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions)
Acetonitrile mecn, by Merck Group (1 mentions)
Fmoc amino acids, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Tio2 nanoparticles, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by HiMedia (1 mentions)
23 protocols using n dimethylformamide dmf
1
Solid-Phase Peptide Synthesis Protocol
2
Synthesis and Characterization of P(VDF-TrFE) Nanocomposite
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P(VDF-TrFE) (70/30 PVDF/TrFE ratio) was supplied by Piezotech SAS, France. TiO2 nanoparticles (Average diameter 25 nm), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and DAPI (4,6-diamidino-2-phenylindole) were purchased from Sigma Aldrich, USA. Phalloidin was obtained from Applied Biosystems, USA. Fetal Bovine Serum (FBS), Antibiotic-Antimycotic solution and Dulbecco’s Modified Eagles Medium (DMEM) were purchased from Himedia, India. Acetone, N, N-Dimethyl formamide (DMF) and Paraformaldehyde was obtained from Merck (India).
3
Synthesis of NH2-H2BDC-based Metal-Organic Framework
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All of the reagents including 2-aminoterephthalic acid (NH2-H2BDC), aluminum chloride hexahydrate (AlCl3. 6H2O), N, N-Dimethylformamide (DMF), and methanol (MeOH) were purchased from Merck Company and utilized without any purification. Disodium hydrogen arsenate (Na2HAsO4.7H2O), hydrochloric acid (HCl), sodium hydroxide (NaOH) was also purchased from Merck Company.
4
Synthesis and Characterization of PVDF-[Bmim]2[NiCl4] Composite
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The polymer PVDF (6010) (300 000-330 000 Da) was purchased from Solvay. The IL
[Bmim]2[NiCl4] (99 % purity) was synthesized as reported in [31] (link)[32] (link) . The solvent N, Ndimethylformamide (DMF) (99.5 % purity) was purchased from Merck.
[Bmim]2[NiCl4] (99 % purity) was synthesized as reported in [31] (link)[32] (link) . The solvent N, Ndimethylformamide (DMF) (99.5 % purity) was purchased from Merck.
5
Chitosan-Based Peptide Synthesis Protocol
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Chitosan 70/1000 was purchased by Heppe Medical Chitosan GmbH (HMC+, Halle, Germany). All Fmoc protected amino acids, resins, coupling reagents 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethyluroniumhexafluorophosphate (HBTU), and Oxima Pure, used for peptide synthesis were from Novabiochem (Merck KGaA, Darmstadt, Germany). Piperidine, diethyl ether, N, N-diisopropylethylamine (DIPEA), trifluoroacetic acid (TFA), and dichloromethane (DCM) were from Biosolve (Leenderweg, Valkenswaard, The Netherlands). All other solvents such as N, N-dimethylformamide (DMF) and reagents as sodium (meta)periodate (NaIO4) were from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany) unless otherwise indicated.
6
Fabrication of Polymer Nanofiber Scaffolds
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Poly (ε-caprolactone) (PCL) or poly (lactic-co-glycolic acid) (PLGA) nanofibers were synthesized as described previously.13 (link)
A homogeneous polymer solution (12% w/v) was prepared by dissolving the polymer in N, N-Dimethylformamide (DMF), and Chloroform (both from Sigma-Aldrich, St. Louis, MO) overnight at room temperature. Microspheres were incorporated within nanofiber scaffolds by combining 10% w/w solutions with complementary polymers, namely PCL microspheres in PLGA solution of PLGA microspheres in PCL solutions. For electrospinning, solutions in a 10 mL syringe with a 20 Gauge metallic needle were used at a high voltage of 30 kV. The flow rate of the polymer solution was 500 µL/h, and the distance between the collector and needle tip was 20 cm. The drum rotational speeds were adjusted to 300 or 700 rpm for aligned and random nanofibers, respectively. Alignment of nanofibers at low speed could be attributed to the elimination of the whipping phase, as noted in prior studies.25–28 (no links found)
A homogeneous polymer solution (12% w/v) was prepared by dissolving the polymer in N, N-Dimethylformamide (DMF), and Chloroform (both from Sigma-Aldrich, St. Louis, MO) overnight at room temperature. Microspheres were incorporated within nanofiber scaffolds by combining 10% w/w solutions with complementary polymers, namely PCL microspheres in PLGA solution of PLGA microspheres in PCL solutions. For electrospinning, solutions in a 10 mL syringe with a 20 Gauge metallic needle were used at a high voltage of 30 kV. The flow rate of the polymer solution was 500 µL/h, and the distance between the collector and needle tip was 20 cm. The drum rotational speeds were adjusted to 300 or 700 rpm for aligned and random nanofibers, respectively. Alignment of nanofibers at low speed could be attributed to the elimination of the whipping phase, as noted in prior studies.25
7
Analytical Characterization of Pharmaceutical Formulations
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In the present work, all reagents used were of an analytical standard and were used without pre-processing. CFT was supplied by Sigma-Aldrich. Spectracef® pediatric sachets, the pharmaceutical dosage form, were obtained from a topical pharmacy. Drug-free human serum from male AB plasma was received from Sigma-Aldrich. Sodium acetate trihydrate (>99%), acetic acid (>99%), phosphoric acid (>85%), acetonitrile (99.8%), methanol (99.8%), sodium dihydrogen phosphate dihydrate (>99%), sodium phosphate monobasic (≥99.0%), sodium phosphate (≥99.0%), sulfuric acid (95–97%), sodium hydroxide (>97%), N, N-dimethyl formamide (DMF), boric acid (>99%), hydrochloric acid (37%), potassium chloride (KCl), magnesium sulphate (MgSO4), sodium nitrate (NaNO3), ascorbic acid, glucose, dopamine, uric acid, and paracetamol were procured from Sigma-Aldrich. Fe2O3, chitosan NCs, and the MWCNTs were obtained from Sigma-Aldrich.
8
Fabrication of Cellulose Nanofiber Reinforced Composites
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Polycaprolactone (molecular weight = 80,000, GPC and melt viscosity ASTM D1238:73); N-dimethylformamide (DMF) (anhydrous, 99.8% w/v); and chloroform (99.8% w/v) were purchased from Sigma-Aldrich (St. Louis, MO, USA) [40 (link)]. Cellulose and nanofiber fibers (CNF) were obtained according to the methodology described in a previous work [7 (link)]. In summary, the agave bagasse fibers of the Agave tequilana Weber Plant (Arenal Jalisco, Mexico) were delignified, degreased, and cut, and the cellulose was then obtained using the organosolv method. The cellulose pulp was then bleached. Subsequently, agave cellulose fibers were processed at 1.5% consistency in an M-110P microfluidizer and, thus, fibrillation and CNF formation occurred.
The reagents used to determine the chemical composition were supplied by Sigma-Aldrich (Toluca, Mexico), i.e., aqueous solutions of acetic acid (CH3COOH 99% w/v); sodium chlorite (NaClO2, 80% w/v); potassium hydroxide (KOH, 90% w/v); sodium hydroxide (NaOH, 97% w/v); and nitric acid (H3NO3, 99.5% w/v). All chemicals were used without further purification.
The reagents used to determine the chemical composition were supplied by Sigma-Aldrich (Toluca, Mexico), i.e., aqueous solutions of acetic acid (CH3COOH 99% w/v); sodium chlorite (NaClO2, 80% w/v); potassium hydroxide (KOH, 90% w/v); sodium hydroxide (NaOH, 97% w/v); and nitric acid (H3NO3, 99.5% w/v). All chemicals were used without further purification.
9
Synthesis of Metallic Nanostructures
10
Biodegradable Polymer Blends Synthesis
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PVA with average molecular weights (MW) ranging from 89,000 to 98,000 g/mol (Product Number: 341584), PHB (Product Number: 363502), and solvents, N-dimethylformamide (DMF); Reagent Plus®, ≥ 99%, chloroform (CF); anhydrous, ≥ 99%; and acetone (Ace) for HPLC, ≥ 99.8% were obtained from Sigma Aldrich. PLLA with a MW of 282,000 g/mol was obtained from Vorina Biomaterials, Ireland (CAS Number: 33135-50-1). Urea (% N: P: K; 46:0:0) from Richgro Garden Products was used in all experiments. A 400/112 denier PVA multifilament yarn was obtained from Dongguan Cocou Textile Materials Company in China.
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