The largest database of trusted experimental protocols

Mn gf 5

Manufactured by Macherey-Nagel
Sourced in Germany

The MN GF-5 is a glass fiber filter manufactured by Macherey-Nagel. It is designed for filtration applications in the laboratory setting.

Automatically generated - may contain errors

5 protocols using mn gf 5

1

Pyruvate Uptake Assay in Salmonella Typhimurium

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine the uptake of pyruvate by S. Typhimurium, a transport assay was performed with radiolabeled pyruvate. Cells were grown under agitation at 37 °C in LB and harvested in mid-log phase. Cells were pelleted at 4 °C, washed twice, and resuspended in transport buffer (1 g/L (NH4)2SO4, 10 g/L K2HPO4, 4.5 g/l KH2PO4, 0.1 g/L MgSO4, pH 6.8) to an absorbance of 5 at 420 nm, equivalent to a total protein concentration of 0.35 mg/mL. Uptake of 14C-pyruvate (55 mCi/mmol, Biotrend, Köln, Germany) was measured at a total substrate concentration of 10 µM at 18 °C. At various time intervals, transport was terminated by the addition of ice-cold stop buffer (100 mM potassium phosphate, pH 6.0, 100 mM LiCl) followed by rapid filtration through membrane filters (MN gf-5, 0.4 μm nitrocellulose, Macherey Nagel, Düren, Germany). The filters were dissolved in 5 mL scintillation fluid (MP Biomedicals, Eschwege, Germany), and radioactivity was determined in a liquid scintillation analyzer (PerkinElmer, Waltham, MA, USA).
+ Open protocol
+ Expand
2

Nitrite Concentration Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Previous to chemical analysis the grab samples were filtered through 0.4 μm filter papers (MN GF-5, MACHERY-NAGEL GmbH & Co. KG, Düren, Germany). The nitrite concentrations were measured with spectrophotometric cuvette tests (LCK 341 with a measurement range of 0.015 – 0.6 mgN/L and LCK 342 with a measurement range of 0.6 – 6 mgN/L, Hach Lange GmbH, Germany) using a spectrophotometer from Hach Lange GmbH (DR 2800, Hach Lange GmbH, Germany). With these cuvette tests, we typically achieve a measurement accuracy of 1%.
+ Open protocol
+ Expand
3

Micropollutant Removal in Low NH4⁺-N Bioreactors

Check if the same lab product or an alternative is used in the 5 most similar protocols
With the aim of investigating whether micropollutant removal
is possible under biological treatment conditions with low
NH4+—N concentrations and high C:N ratios, two 12-L
sequencing batch reactors were run in series down-stream of a full-scale
municipal activated sludge process. The full-scale plant contained three
equally sized compartments (one anoxic and two oxic, 2 mg/L
O2) and was operated with 1 d HRT, ∼25 d SRT, ∼2 g/L SS. The
two 12-L post-treatment reactors were operated in series with carriers (K1,
AnoxKaldnes; 40% fill ratio), a HRT of 24 h per reactor and three batches per
day. In the first post-treatment reactor, the batch cycle contained two
reaction phases: one oxic (5–6 h) with 0.5–2.5 mg/L O2, and
one anoxic (1–2 h) with acetate addition (40 mg/L DOC). In the second
post-treatment reactor, the batch cycle contained one oxic reaction phase
(7–8 h) with 0.2–1.5 mg/L O2 and acetate addition (110 mg/L
DOC).
A 3-week sampling campaign was conducted six months after
reactor start-up. Daily flow proportional samples of the influent and effluent
from each reactor were collected, filtered (MN GF-5, 0.4 μm, Macherey-Nagel)
and stored at 6 °C until the end of each sampling week. The samples were then
mixed proportionally to the flow to obtain 7-day composite samples and stored
at −20 °C until analysis.
+ Open protocol
+ Expand
4

Synthesis and Characterization of Ettringite Precipitate

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following Struble et al. [20 ], a solution of aluminium sulphate octadecahydrate [Al2(SO4)3·18H2O, 38.3 mM, 1 Veq.] and a saturated solution of calcium hydroxide [Ca(OH)2, 10 Veq.] in water were prepared. The alkaline solution was filtrated using a Buchner funnel with a glass fibre filter (retention 0.4 µm, d = 125 mm; MN-GF 5 by Macherey-Nagel GmbH, Co. KG, Dueren, Germany). Both solutions were mixed quickly (30 s) under vigorous stirring (500 rpm, Hei-Torque Precision 400 by Heidolph Instruments GmbH, Co. KG; anchor AR 19 PTFE, dshaft = 8 mm). Instantaneous precipitation of ettringite was evident from the solution turning turbid. After centrifugation (60 min, 10,000 G, Sigma 3-18KS, 20 °C), the precipitate was decanted. To remove the adsorbed water, or rather remaining reaction medium, with neither phase transition (25 °C, 1.015 bar) nor the formation of lime [CaCO3] (Ar atmosphere), the precipitate was dried in a vacuum furnace (Vakuumschrank VO29 by Memmert GmbH + Co. KG, Büchenbach, Germany) for 24 h at the aforementioned ambient and inert conditions.
+ Open protocol
+ Expand
5

Allelopathic Effects on Microalgae

Check if the same lab product or an alternative is used in the 5 most similar protocols
Allelopathic effects were tested according to a method proposed by Suikkanen et al. [19 (link)], with modifications. Experimental treatments were prepared by adding 10 mL of the cell-free filtrate (f/2 medium in controls) to 25-mL Erlenmeyer flasks containing 10 mL of cell suspensions of the targeted species. In all experiments, the initial Chl a concentration in the cultures was 0.4 µg Chl a mL−1. Synechococcus sp. strains were filtered through a 0.45-µm filter (Macherey-Nagel MN GF-5). Tests were conducted in triplicate and the results of the experiments are presented as the mean value of three independent measurements.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!