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Ufp101

Manufactured by Bio-Techne
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Sourced in United States

The UFP101 is a high-performance centrifuge designed for a variety of laboratory applications. It features a compact and durable design with a maximum speed of 10,000 rpm and a maximum RCF of 12,100 x g. The centrifuge accommodates rotor sizes up to 100 mL and is suitable for a wide range of sample volumes and applications.

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Lab products found in correlation

Cobalt chloride, by Merck Group (1 mentions) Desferrioxamine, by Merck Group (1 mentions) Tocopherol, by Merck Group (1 mentions) Necrosulfonamide, by Merck Group (1 mentions) Hydroxychloroquine sulfate, by Merck Group (1 mentions) Lapatinib, by Selleck Chemicals (1 mentions) Crystal violet, by Merck Group (1 mentions) P rela, by Cell Signaling Technology (1 mentions) Hmgb1, by Novus Biologicals (1 mentions) Jtc 801, by Selleck Chemicals (1 mentions) Hydrogen peroxide solution, by Merck Group (1 mentions) P iκbα, by Cell Signaling Technology (1 mentions) Tumor necrosis factor (tnf), by Merck Group (1 mentions) Sb 612111, by Bio-Techne (1 mentions) Cleaved parp 1, by Cell Signaling Technology (1 mentions) Ferrostatin 1, by Selleck Chemicals (1 mentions) Z vad fmk, by Merck Group (1 mentions) Cytochalasin b, by Merck Group (1 mentions) Gemcitabine hydrochloride, by Merck Group (1 mentions) P ikkβ, by Cell Signaling Technology (1 mentions)

4 protocols using ufp101

1

Comprehensive Immunoblot Analysis

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The antibodies to CA9, p-RelA, RelA, p-IKKβ, IKKβ, p-IκBα, IκBα, LDH, HIF1α, SQSTM1, cleaved-caspase-3, cleaved-PARP1, NRF2, and actin were obtained from Cell Signaling Technology. The antibodies to GPX4 and CA9 were obtained from Abcam. The antibodies to LC3, MLKL, RIPK3, OPRL1, OPRM1, and HMGB1 were obtained from NOVUS. Desferrioxamine, β-mercaptoethanol, N-acetyl-l-cysteine, N-Acetyl-l-alaine, hydrogen peroxide solution, gemcitabine hydrochloride, sulfasalazine, cobalt chloride, cycloheximide, tocopherol, necrosulfonamide, hydroxychloroquine sulfate, EDTA, cytochalasin B, cytochalasin D, paclitaxel, crystal violet, protease inhibitor cocktail, Z-VAD-FMK, TNF, staurosporine, cycloheximide, and lipopolysaccharides were obtained from Sigma-Aldrich. Erastin, ferrostatin-1, lapatinib, JTC801, and compound libraries were obtained from Selleck Chemicals. BANORL24, SB612111, UFP101, and Trap101 were obtained from Tocris Bioscience.
Song X., Zhu S., Xie Y., Liu J., Sun L., Zeng D., Wang P., Ma X., Kroemer G., Bartlett D.L., Billiar T.R., Lotze M., Zeh H., Kang R, & Tang D. (2017). JTC801 Induces pH-dependent Death Specifically in Cancer Cells and Slows Growth of Tumors in Mice. Gastroenterology, 154(5), 1480-1493.
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2

Pharmacological Agents in Neuroscience Research

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(−) Bicuculine methiodide (#2503) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; #0190) purchased from Tocris Bioscience (Ellisville, MO, USA) dissolved in dimethyl sulfoxide (DMSO), tetrodotoxin (#1069) and UFP-101 (#1552) from Tocris Bioscience (Ellisville, MO, USA) dissolved in water, and Ro 64-6198 (#SML1936) from Sigma-Aldrich (St. Louis, MO, USA) dissolved in DMSO were used in this study. The final concentration of DMSO was controlled to <0.1% of water. BTRX-246040 (LY2940094, #T15799, TargetMol) dissolved in saline (3 mg/mL) with 1% DMSO and 30% sulfobutylether-β-cyclodextrin was used in this study (after ultrasonication). The stock solution was subsequently diluted in saline. Saline with 1% DMSO and 30% cyclodextrin was used as the vehicle (Veh).
Li Z., Xu Y., Li R., Sheng Z., Chen X., Liu X., Lee C.S., Peng H.Y., Lin T.B., Hsieh M.C., Lai C.Y., Wu H.F, & Chou D. (2023). BTRX-246040 Acts Through the Ventrolateral Periaqueductal Gray to Exert Antidepressant-Relevant Actions in Mice. International Journal of Neuropsychopharmacology, 26(7), 483-495.
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3

Pharmacological Modulation of Neuronal Signaling

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Cesium (Ca), barium [Ba; both broad-spectrum blockers of inwardly rectifying potassium (Kir) channels], naringin (GIRK channel activator), phorbol 12-myristate 13-acetate [PMA; protein kinase C (PKC) activator], and baclofen (BAC; GABAB receptor agonist) were purchased from Sigma-Aldrich. 3-isobutyl-1-methylxanthine (IBMX; inhibitor of phosphodiesterase), forskolin (FSK; activator of adenylyl cyclase), 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX; AMPA/kainate receptor antagonist), d(−)-2-amino-5-phosphonopentanoic acid (d-AP5; NMDA receptor antagonist), bicuculline (BIC; GABAA receptor antagonist), TPNQ (blocker of GIRK channels), UFP-101 (UFP) and SB 162111 (SB; two selective antagonists for ORL1 receptor), PTX (uncoupling Gi/o-protein-coupled receptor), ML297 (GIRK channel activator) and human kisspeptin-10 [kp-10; Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2] were purchased from Tocris Bioscience. Nociceptin/orphanin FQ (1-13)-NH2 [OFQ; Phe-Gly-Gly-Phe-Thr-Gly-Ala-Arg-Lys-Ser-Ala-Arg-Lys-NH2] was purchased from Phoenix. All stock solutions (1000× or 500×) were stored at −20°C and diluted prior to each experiment at the specified concentration in SFM.
Note that throughout the calcium-imaging and electrophysiology result sections, OFQ refers to the truncated form, OFQ(1-13)-NH2, exogenously applied.
Constantin S, & Wray S. (2018). Nociceptin/Orphanin-FQ Inhibits Gonadotropin-Releasing Hormone Neurons via G-Protein-Gated Inwardly Rectifying Potassium Channels. eNeuro, 5(6), ENEURO.0161-18.2018.
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4

Intrathecal Nociceptin Modulation of Cardiovascular Function

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The sealed end of the implanted subarachnoid catheter was retrieved and was flushed with 3 μL of aCSF to ensure patency. Nociceptin,37 (link) a selective agonist of NOP (1, 2, 5 nmol; Tocris Bioscience, Bristol, UK), was given at a volume of 3 μL over 1 minute using a 25 μL Hamilton microsyringe, followed by an additional 3 μL of aCSF to flush the catheter. In experiments that engaged the specific antagonist,36 (link) UFP101 (10 nmol; Tocris Bioscience) was similarly delivered intrathecally, together with Nociceptin (5 nmol) or aCSF. The doses used were adopted from the relevant literature.36 (link),37 (link) The effects of drug treatments on MAP, HR, BLF power, and BRS were routinely followed for 60 minutes after administration, and the effects on tail-flick responses were evaluated 30 minutes after intrathecal injection of Nociceptin or UFP101 plus Nociceptin. Injection of 3 μL of aCSF served as the vehicle control for Nociceptin and UFP101.
Tsai C.Y., Poon Y.Y., Huang Y.H, & Chan S.H. (2018). Activation of spinal nociceptin receptors induces cardiovascular depression and antinociception in an independent manner in mice. Journal of Pain Research, 11, 2699-2708.
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