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Mouse magnetic luminex assay for igf 1

Manufactured by R&D Systems

The Mouse Magnetic Luminex Assay for IGF-1 is a multiplex assay designed to quantitatively measure insulin-like growth factor 1 (IGF-1) levels in mouse samples. The assay utilizes magnetic beads coated with antibodies specific to IGF-1, which capture the analyte in the sample. The bound IGF-1 is then detected using a fluorescent reporter molecule.

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2 protocols using mouse magnetic luminex assay for igf 1

1

Measuring IGF-1 and GH in Murine Plasma

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Plasma from blood was collected in the morning at fixed time on EDTA from 1, 5 and 15 day-old mice after euthanasia and stored at –20°C until use. Samples were run in duplicates using the Mouse Magnetic Luminex Assay for IGF-1 (R&D System) and Milliplex Mouse Pituitary Magnetic Assay for GH (Merck) according to manufacturers’ instructions. Values were read on Bio-Plex 200 (Bio-Rad) and analyzed with the Bio-Plex Manager Software.
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2

Murine Plasma Hormone Analysis

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Plasma from blood was collected in the morning at fixed time on EDTA from 5 and 15 dayold mice after euthanasia and stored at -20C until use. Samples were run in duplicates using the Mouse Magnetic Luminex Assay for IGF-1 (R&D System) and Milliplex Mouse Pituitary Magnetic Assay for GH (Merck) according to manufacturers' instructions. Values were read on Bio-Plex® 200 (Bio-Rad) and analyzed with the Bio-Plex Manager Software.
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