Eia plate reader

Plasma levels of IFABP, CCL25, IL‐18, IL‐18 binding protein (IL‐18BP) and LBP were measured in duplicate by enzyme immunoassays (EIA) using commercially available antibodies (R&D Systems, Minneapolis, MN, USA) in a 384 format using a combination of a SELMA (Jena, Germany) pipetting robot and a BioTek (Winooski, VT, USA) dispenser/washer. Absorption was read at 450 nm with wavelength correction set to 540 nm using an EIA plate reader (Bio‐Rad, Hercules, CA, USA). Plasma levels of IL‐1β and IL‐1Receptor antagonist (IL‐1Ra) were analysed using a multiplex cytokine assay (Bio‐Plex Human Cytokine 27‐Plex Panel; Bio‐Rad Laboratories Inc., Hercules, CA, USA). The samples were analysed on a Multiplex Analyzer (Bio‐Rad Laboratories) according to the manufacturer’s instructions.

Plasma levels of IFABP, CCL25, IL-18, and LBP were measured in duplicate by enzyme immunoassays (EIA) using commercially available antibodies (R&D Systems, Minneapolis, MN, USA) in a 384 format using a combination of a SELMA (Jena, Germany) pipetting robot and a BioTek (Winooski, VT, USA) dispenser/washer. Absorption was read at 450 nm with wavelength correction set to 540 nm using an EIA plate reader (Bio-Rad, Hercules, CA, USA). All samples for a marker were run on the same 384 plate and intrassay coefficient of variation was < 10%.