The morphology of NE was determined with a FEI Tecnai G2 20S-Twin 200 kV high-resolution transmission electron microscope (HRTEM) (Brno, Czech Republic, EU) equipped with an Oxford INCA X-Sight EDS System. Sample preparation diluted a small volume of NE in ultrapure water. The dilution was placed on a microscopic carbon-coated 300 mesh copper grid and left to dry for 10 min; thereafter, the grid was stained with osmium tetroxide, and after allowing another 20–30 min for drying, the grid was analyzed at a voltage of ±200 kV. A Gatan bottom-mount camera with digital micrograph software (DigitalMicrograph 3.5) captured micrographs at different magnifications [38 (link)].
Tecnai g2 20s twin 200 kv
Manufactured by Thermo Fisher Scientific
Sourced in Czechia, United States
The Tecnai G2 20S-Twin 200 kV is a high-performance transmission electron microscope (TEM) designed for advanced imaging and analysis. It features a 200 kV electron beam and a Twin lens system for improved resolution and contrast. The core function of this instrument is to enable high-resolution imaging and characterization of a wide range of samples, including materials, biological specimens, and nanostructures.
Automatically generated - may contain errors
2 protocols using tecnai g2 20s twin 200 kv
1
NE Morphology Characterized by HRTEM
2
Characterization of Electrospun Nanofibers and PC12 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Morphological characterisation of the electrospun nanofibres and cultured PC12 was carried out using a Zeiss SIGMA FEG-SEM Scanning Electron Microscope [FEG-SEM].
Transmission electron microscopy was carried out using a TECNAI G2 20 S-TWIN (200kV); Resolution: 2.4A0, FEI COMPANY, USA, transmission electron microscope (TEM). XPS was performed using an ESCALAB 250 Xi system (Thermo Scientific) equipped with a monochromated Al Kα X-ray source. Uniform charge neutralization was provided by beams of low-energy (≤10 eV) Ar+ ions and low-energy electrons guided by the magnetic lens. The standard analysis spot of ca. 900×600 µm 2 was defined by the microfocused X-ray source.
Full survey scans (step size 1 eV, pass energy 150 eV, dwell time 50mS and 3 scans) and narrow scans (step size 0.1 eV, pass energy 20 eV, dwell time 100 mS and 5 scans) of the C1s 7 (BE ∼285 eV) regions were acquired from three separate areas on each sample. Data were transmission function corrected and analysed using Thermo Avantage Software (Version 5.952) using a smart background. FTIR spectra were recorded using a Nicolet Impact I-410 Spectrometer (SpectraLab Scientific Inc., USA). Confocal microscopy was carried out using Leica TCS SP5 Confocal Laser Scanning Microscope [CLSM] (Leica Microsystems, UK).
For stability test, SEM was performed using JSM 6390LV, JEOL, Japan.
Transmission electron microscopy was carried out using a TECNAI G2 20 S-TWIN (200kV); Resolution: 2.4A0, FEI COMPANY, USA, transmission electron microscope (TEM). XPS was performed using an ESCALAB 250 Xi system (Thermo Scientific) equipped with a monochromated Al Kα X-ray source. Uniform charge neutralization was provided by beams of low-energy (≤10 eV) Ar+ ions and low-energy electrons guided by the magnetic lens. The standard analysis spot of ca. 900×600 µm 2 was defined by the microfocused X-ray source.
Full survey scans (step size 1 eV, pass energy 150 eV, dwell time 50mS and 3 scans) and narrow scans (step size 0.1 eV, pass energy 20 eV, dwell time 100 mS and 5 scans) of the C1s 7 (BE ∼285 eV) regions were acquired from three separate areas on each sample. Data were transmission function corrected and analysed using Thermo Avantage Software (Version 5.952) using a smart background. FTIR spectra were recorded using a Nicolet Impact I-410 Spectrometer (SpectraLab Scientific Inc., USA). Confocal microscopy was carried out using Leica TCS SP5 Confocal Laser Scanning Microscope [CLSM] (Leica Microsystems, UK).
For stability test, SEM was performed using JSM 6390LV, JEOL, Japan.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!