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2 protocols using o cyanobenzoic acid

1

Extraction and Dissolution of Bacterial Metabolites

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Identified bacterial metabolites such as Phthalimide (PM), o-Cyanobenzoic acid (CBA), and 3-Ethoxy-4-Methoxyphenol (EMP) were obtained from Sigma-Aldrich Korea (Seoul, Korea) and dimethylsulfoxide (DMSO) was used to dissolve these. Trizol reagent was collected from Invitrogen Korea (Seoul, Korea) and used for RNA extraction. Chloroform and Ethanol were collected from Sigma-Aldrich Korea (Seoul, Korea). Phosphate-buffered saline (PBS, pH 7.4) was prepared in the laboratory with 100 mM phosphoric acid and 0.7% sodium chloride. Anticoagulant buffer (ACB, pH 4.5) containing 186 mM NaCl, 17 mM Na2EDTA, and 41 mM citric acid was prepared in the laboratory.
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2

Synthesis and Characterization of Bioactive Compounds

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Phthalimide (PM), 3-Ethoxy-4-methoxyphenol (EMP), o-cyanobenzoic acid (CBA), dibutylamine (DBA), bis-2 (ethylhexyl) phthalate (BEP), indole (IND), hexahydro-3-(2-methylpropyl)-pyrrolopyrazine-1,4-dione (HMPP), and salicylic acid (SA) were purchased from Sigma-Aldrich Korea (Seoul, Korea) and dissolved in dimethylsulfoxide (DMSO). Phosphate-buffered saline (PBS, pH 7.4) was prepared with 100 mM phosphoric acid and 0.7% sodium chloride. Anticoagulant buffer (ACB, pH 4.5) was prepared to contain 186 mM NaCl, 17 mM Na2EDTA, and 41 mM citric acid. A transfection reagent (Metafectene Pro) was purchased from Biontex (Plannegg, Germany).
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