Serum, lung, liver, and kidneys were harvested from Cl-13-infected mice. Serum was isolated from whole blood collected in heparinized tubes (Fisher; Pittsburgh, PA), capped (Lecia Microsystems; Richmond, IL) and spun at 2500 rpm for 30 min. Serum was collected and snap-frozen in liquid nitrogen prior to storage at -80°C. Lung, liver and kidneys were homogenized and processed for plaque assays as previously described [48 (link)].
Heparinized tubes
Manufactured by Thermo Fisher Scientific
Heparinized tubes are specialized laboratory containers used for the collection and storage of blood samples. Their primary function is to prevent the coagulation or clotting of the collected blood, allowing for accurate analysis and testing of the sample.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using heparinized tubes
1
Virus Tissue Harvesting and Quantification
2
Plasma Metabolite Analysis in Fed Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Approximately 20 µl of whole blood was obtained by submandibular bleed at 9–10 a.m. from mice in a fed state. Blood was collected on ice using heparinized tubes (Fisherbrand) and plasma was isolated by centrifugation for 20 min at 2000 × g at 4 °C, aliquoted, and frozen at −80 °C until time of assay. Plasma leptin concentration was measured using mouse leptin ELISA (R&D) with sensitivity of 22 pg/ml. Plasma insulin concentration was measured using mouse insulin ELISA (Mercodia) and plasma glucose was measured with Autokit Glucose (WAKO).
3
Plasma Collection and Metabolic Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was collected on ice using heparinized tubes (Fisherbrand). Plasma was isolated by centrifugation for 20 min at 2,000 x g at 4°C and frozen at −80°C until assay. Mice were bled at 11 weeks of age prior to transfer to the calorimeters (Fig 1
4
Plasma Biomarker Measurements in Mice
5
Evaluating Liver Toxicity in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6J mice (n = 5–7) were treated i.v. with CPMV, CPMV‐H6, CPMV‐G3, H6, G3, or PBS (200 mg CPMV per mouse; the peptide dose was normalized to match the number of peptides delivered by CPMV). After 1, 3, and 7 days, blood was collected through retroorbital bleeds using heparinized tubes (Fisher Scientific). The blood was spun down at 7500 rpm for 10 min at 4 °C and the sera was collected and stored at −80 °C. The sera were then subjected to aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activity testing by following the manufacturer's guidelines (Abcam). Briefly, the sera were diluted 10x, and compared against a standard curve of pyruvate and glutamate for the ALT and AST assay, respectively. Fluorometric readings at 535 nm (excitation) and 587 nm (emission) and at 10 and 40 min were used to measure ALT activity while AST activity was measured using absorbance readings at 450 nm also at 10 and 40 min (Tecan plate reader).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!