The samples and reference material were acid-digested with nitric acid (69%; Suprapur®; Carl Roth, Karlsruhe, Germany) in a microwave oven (MARS6, CEM Corporation, Matthews, NC, USA). The samples, stabilized with HCl, were stored at 4 °C for up to three days and diluted in a ratio of 1:2.5 before they were analyzed for total mercury content by cold vapour atomic fluorescence spectroscopy (CV-AFS) (Mercur Plus, Analytik Jena AG, Jena, Germany). Quality control was achieved by measuring blank test solutions (limit of detection was 0.024 µg/L) and reference materials (Seronorm Trace Elements Urine L-2, 210705, LOT 1011645). The mercury levels of the reference material (30.6 ± 7.2 µg/L; n = 19) lay well within the certified range (23.8–55.8 µg/L). All samples were measured in duplicate by the working curve method (RSD < 15%).
Mercur plus
Manufactured by Analytik Jena
Sourced in Germany
The Mercur Plus is a high-performance mercury analyzer designed for the accurate and precise determination of total mercury content in a wide range of sample matrices. It utilizes advanced atomic absorption spectroscopy technology to provide reliable and reproducible results.
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Lab products found in correlation
5 protocols using mercur plus
1
Mercury Content Analysis in Samples
2
Quantification of Methylmercury in Samples
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Cells and reference material (trace elements urine L-2, solder 1403081) were digested with nitric acid (69%; Suprapur; Roth; HN50.3, Karlsruhe, Germany) in a microwave oven (MARS6, CEM Corporation; Matthews, NC, USA) and analyzed for total MeHg by atomic fluorescence spectroscopy (AFS; mercur plus, Analytic Jena, Jena, Germany). The concentrations of the reference material (46.44 ± 0.53 µg/L; n = 2; recovery 105 ± 1%) were within the certified range (Hg: 44.0 µg/L, range: 35.2–52.9 µg/L). The detection limit was 0.016 µg/L (n = 3). All samples were measured in duplicate (RSD < 1%) at the appropriate dilution and the concentrations were calculated from a standard curve (0.0–3.2 µg/L MeHg).
3
Cold Vapor AFS for Mercury Analysis
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Total mercury levels were measured using Mercur Plus, a cold vapor atomic fluorescence spectrophotometry analyzer (Analytik Jena, Jena, Germany) equipped with a low-pressure mercury lamp as the radiation source (λ = 253.7 nm). The instrumental conditions were the same as reported by Yánez-Jácome et al., [28 ].
4
Cold Vapor Atomic Fluorescence Mercury Analysis
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All measurements were carried out using Mercur Plus, a cold vapor analyzer based on atomic fluorescence (Analytik Jena, Jena, Germany). A low-pressure mercury lamp UVU5 was used as a radiation source. Measurements were carried out with an excitation and fluorescence wavelength of 253.7 nm.
5
In Vitro MeHg Quantification Protocol
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In vivo MeHg demethylation rate is very low (about 1% of body exposure per day) (Clarkson 2002 (link)). It must therefore be assumed that MeHg is practically not demethylated in in vitro experiments lasting a few days. Thus, the MeHg contents in supernatants and cells were analyzed as total Hg.
Cells, medium and reference material (Trace Elements Urine L-2, Lot 1403081) were digested with nitric acid (69%; Suprapur®; Roth; HN50.3) in a microwave oven (MARS6, CEM Corporation) and analyzed for total Hg by atomic fluorescence spectroscopy (mercur plus, Analytic Jena). The concentrations of the reference material (42.4 ± 0.98; n = 3; Recovery 96 ± 2%) lay well within the certified range (Hg: 44.0 µg/L, range: 35.2–52.9 µg/L). The limit of detection was 0.012 µg/L (n = 3). All samples were measured in duplicate (RSD < 1%) in the appropriate dilution and concentrations were calculated from a standard curve (0.0–3.2 µg/L).
Cells, medium and reference material (Trace Elements Urine L-2, Lot 1403081) were digested with nitric acid (69%; Suprapur®; Roth; HN50.3) in a microwave oven (MARS6, CEM Corporation) and analyzed for total Hg by atomic fluorescence spectroscopy (mercur plus, Analytic Jena). The concentrations of the reference material (42.4 ± 0.98; n = 3; Recovery 96 ± 2%) lay well within the certified range (Hg: 44.0 µg/L, range: 35.2–52.9 µg/L). The limit of detection was 0.012 µg/L (n = 3). All samples were measured in duplicate (RSD < 1%) in the appropriate dilution and concentrations were calculated from a standard curve (0.0–3.2 µg/L).
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