Egrf tyr845p

Manufactured by Cell Signaling Technology

Sourced in United States

EGRF-Tyr845P is an antibody that specifically recognizes the phosphorylated form of the epidermal growth factor receptor (EGFR) at tyrosine residue 845. It is a tool used in research to detect and study the activation of EGFR signaling pathways.

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3 protocols using egrf tyr845p

Immunoblot Analysis of Protein Expression

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For immunoblots, equal total protein of cell lysates (2.5–20 μg) were detected with antibodies to mouse p53 (FL393), human p53 (PAb1801; Santa Cruz Biotechnology), HSF1, p-Ser326 HSF1, AKT, pAKT, Erk, pErk, Hsp70, Hsp90, Hsp90α, EGFR, EGRF-Tyr845P (all Cell Signaling, Danvers, MA, USA), ErbB2, actin, GAPDH, GTS, GFP, HSc70 and HDAC1 (all Neomarkers, Fremont, CA, USA).^{35 (link), 36 (link), 37 (link)} SDS-PAAG gels (6%) were used to detect slower migrating HS-activated HSF1 used in experiments presented in Figures 2 and 3. SDS PAAG (10%) gels were used in all other experiments. Co-immunoprecipitations were performed with 1 μg of antibody overnight. Beads were washed with SNNTE and RIPA buffers 3 × each, followed by blotting.

Li D., Yallowitz A., Ozog L, & Marchenko N. (2014). A gain-of-function mutant p53–HSF1 feed forward circuit governs adaptation of cancer cells to proteotoxic stress. Cell Death & Disease, 5(4), e1194-.

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Immunoblot Protein Expression Analysis

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For immunoblots, equal total protein of cell lysates (2.5–20 μg) were detected with antibodies to mouse p53 (FL393), human p53 (PAb1801), MDM2, GAPDH, HSC 70 (all Santa Cruz Biotechnology), Erk, pErk (T202/Y204), EGFR, EGRF-Tyr845P, ErbB2, pErbB2 (Y1221/1222) (all Cell Signaling), HSF1, pSer326 HSF1, Hsp90 (Enzo Life Sciences, Inc., Farmingdale, NY).

Li D, & Marchenko N.D. (2016). ErbB2 inhibition by lapatinib promotes degradation of mutant p53 protein in cancer cells. Oncotarget, 8(4), 5823-5833.

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Protein Expression Analysis by Immunoblot

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For immunoblots, equal total protein of cell lysates (2.5–20μg) were detected with antibodies to mouse p53 (FL393), human p53 (PAb1801) and ALDH 1/2 (all Santa Cruz Biotechn), AKT, pAKT, Erk, pErk, EGFR, EGRF-Tyr845P (all Cell Signaling), ErbB2, actin (all Neomarkers).

Yallowitz A., Li D., Lobko A., Nemajerova A, & Marchenko N. (2015). Mutant p53 amplifies Epidermal Growth Factor Receptor family signaling to promote mammary tumorigenesis. Molecular cancer research : MCR, 13(4), 743-754.

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