Mass spectra in positive and negative ion mode were acquired on an Agilent 6538 UHD and accurate mass Q-TOF/MS. After separation on HPLC system (Agilent 1290 Infinity UPLC, Agilent Technologies, USA), mass spectra were achieved on Agilent 6538 UHD and Accurate-Mass Q-TOF/MS (Agilent Technologies, USA) with the following conditions: electrospray ion source (Dual ESI+); capillary pressure, 3500 V; atomized gas pressure, 45 psi; dry gas temperature, 350 °C; debris voltage, 120 V; data acquisition range, m/z100–1100. Before the experiment, TOF/MS automatically corrected the mass coordinate axis by real-time injection of reference solution (Agilent Technologies, USA), and the flow rate of reference solution was 100 μL/min; Negative ion mode detection: electrospray ion source (Dual ESI); other conditions are the same as positive ion mode.
Agilent 6538 uhd
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 6538 UHD is a high-resolution accurate-mass quadrupole time-of-flight (Q-TOF) mass spectrometer. It is designed for high-performance liquid chromatography-mass spectrometry (HPLC-MS) applications, providing accurate mass measurements and high sensitivity across a wide mass range.
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2 protocols using agilent 6538 uhd
1
Accurate-Mass Q-TOF/MS Analysis of Compounds
2
Serum Metabolic Profiling by UPLC-QTOF/MS
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A total of 100 μL serum was added to 300 μL methanol (Merck & Co., Inc, Whitehouse Station, NJ, USA), the supernatant was collected after vortexed and centrifuged.
Serum metabolic profiling was performed using a Waters ACQUITY UPLC system (Waters Corp., Milford, MA, USA) coupled to Agilent 6538 UHD and Accurate-Mass Q-TOF/MS (Waters). Liquid chromatography conditions were as follows: mobile phase A was a 0.1% aqueous formic acid solution (Merck & Co., Inc.), mobile phase B was acetonitrile (0.1% formic acid), and a gradient elution was used to equilibrate the ACQUITY UPLC@HSS T3 column (100Å, 1.8 μm, 2.1 mm; Waters). The column temperature was set to 40°C, the flow rate was 350 μL/minute, and a total of 4 μL was injected into the system.
Serum metabolic profiling was performed using a Waters ACQUITY UPLC system (Waters Corp., Milford, MA, USA) coupled to Agilent 6538 UHD and Accurate-Mass Q-TOF/MS (Waters). Liquid chromatography conditions were as follows: mobile phase A was a 0.1% aqueous formic acid solution (Merck & Co., Inc.), mobile phase B was acetonitrile (0.1% formic acid), and a gradient elution was used to equilibrate the ACQUITY UPLC@HSS T3 column (100Å, 1.8 μm, 2.1 mm; Waters). The column temperature was set to 40°C, the flow rate was 350 μL/minute, and a total of 4 μL was injected into the system.
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