Cell apoptosis was analyzed by Annexin V-PE Cell Apoptosis Detection Kit (Beyotime Biotechnology, Shanghai, China). Following washing with PBS, transfected cells at the density of 5 × 104 cells/ml were re-suspended using Annexin V-PE (195 µl). Afterward, 7-AAD (5 µl) was added and incubated for 20 min in dark. Subsequently, cell apoptosis was detected using a flow cytometry.
Annexin 5 pe cell apoptosis detection kit
Manufactured by Beyotime
Sourced in China
The Annexin V-PE Cell Apoptosis Detection Kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death, in cell samples. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and the fluorescent dye Propidium Iodide to identify cells undergoing apoptosis.
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2 protocols using annexin 5 pe cell apoptosis detection kit
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Annexin V-PE Cell Apoptosis Assay
2
Apoptosis Quantification via Flow Cytometry
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After 24 h of cell transfection to observe the transfection efficiency, apoptosis was measured using the Annexin V-Phycoerytirin (Annexin V-PE) cell apoptosis detection kit (C1065, Beyotime Biotechnology, China). These data were acquired by flow cytometry (Thermo Fisher, USA) and analyzed by Flowjo software.
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