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Pcag gw v5 hygro

Manufactured by Thermo Fisher Scientific
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The PCAG-GW-V5-Hygro is a lab equipment product manufactured by Thermo Fisher Scientific. It is a plasmid vector designed for gene expression studies. The core function of this product is to facilitate the cloning and expression of gene(s) of interest in mammalian cell lines.

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Lab products found in correlation

Neon transfection system, by Thermo Fisher Scientific (2 mentions) Hygromycin b, by Thermo Fisher Scientific (2 mentions) Lr recombination reaction, by Thermo Fisher Scientific (2 mentions)

2 protocols using pcag gw v5 hygro

1

V5-tagged Protein Expression and IP in mES Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For V5-tagged protein expression and immunoprecipitation, mouse ES cells were electroporated using the Neon transfection system (Invitrogen) with an episomally-replicating vector (pCAG-GW-V5-Hygro) encoding expression of a C-terminal V5 tagged ORF driven by a CAG promoter. ORFs were obtained from the DNASU plasmid repository as Gateway entry clones and inserted into pCAG-GW-V5-Hygro using an LR recombination reaction (Invitrogen). Transfected cells were selected on 125ug/mL Hygromycin B (Invitrogen) to generate stably expressing lines.
McHugh C.A., Chen C.K., Chow A., Surka C.F., Tran C., McDonel P., Pandya-Jones A., Blanco M., Burghard C., Moradian A., Sweredoski M.J., Shishkin A.A., Su J., Lander E.S., Hess S., Plath K, & Guttman M. (2015). The Xist lncRNA directly interacts with SHARP to silence transcription through HDAC3. Nature, 521(7551), 232-236.
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2

V5-tagged Protein Expression and IP in mES Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For V5-tagged protein expression and immunoprecipitation, mouse ES cells were electroporated using the Neon transfection system (Invitrogen) with an episomally-replicating vector (pCAG-GW-V5-Hygro) encoding expression of a C-terminal V5 tagged ORF driven by a CAG promoter. ORFs were obtained from the DNASU plasmid repository as Gateway entry clones and inserted into pCAG-GW-V5-Hygro using an LR recombination reaction (Invitrogen). Transfected cells were selected on 125ug/mL Hygromycin B (Invitrogen) to generate stably expressing lines.
McHugh C.A., Chen C.K., Chow A., Surka C.F., Tran C., McDonel P., Pandya-Jones A., Blanco M., Burghard C., Moradian A., Sweredoski M.J., Shishkin A.A., Su J., Lander E.S., Hess S., Plath K, & Guttman M. (2015). The Xist lncRNA directly interacts with SHARP to silence transcription through HDAC3. Nature, 521(7551), 232-236.
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