Boscalid

Technical-grade resveratrol (a.i. 99%), psoralen (98%), camptothecin (97%), reserpine (98%), and eugenol (99%) were purchased from Shanghai Maclean Biochemical Technology Co., LTD (Shanghai, China). Salicylic acid was provided by Beijing Solarbio Technology Co., LTD. Chalcone (97%) and anethene (99%) were obtained from Biochemical Technology Co., Ltd. (Shanghai, China). Flavanones (98%) and farnesol (mixture of isomers) (SG 0.89) were obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). The fungicides and inhibitor of the alternative respiratory pathway used were azoxystrobin (98.0%, Syngenta Biotechnology, Shanghai), salicylhydroxamic acid (SHAM) (99%, Shanghai Marklin Biochemistry, China), boscalid (99%, BASF Corporation, Nanjing, China), fluazinam (98.4%, Ishihara Sangyo Kaisha Ltd., Japan), fludioxonil (97.9%, Syngenta Biotechnology, Co., Ltd, China), difenoconazole (98.4%, Yulong Chemical Industrial Co., Ltd., Hangzhou), pyrimethanil (93%, Jiangsu Limin Chemical Industrial Co., Ltd., China), and prochloraz (98%, Jiangsu Huifeng Agrochemical Co., Ltd., Dafeng, China), and all PSMs were dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution (1 × 10⁵ µg/mL) and kept in darkness at −20°C.

The experimental greenhouse plot was divided into six sub-plots with chemical application and one sub-plot for control without pesticide spraying. Treatments were carried out with fungicides: Amistar Opti 480 SC (containing active ingredients (a.i.): 80 g a.i./L azoxystrobin, 400 g a.i./L chlorothalonil; Syngenta), Signum 33 WG (267 g a.i./kg boscalid, 67 g a.i./kg pyraclostrobin; BASF), and Switch 62.5 WG (375 g a.i./kg cyprodinil, 250 g a.i./kg fludioxonil; Syngenta) at fruiting stage (BBCH code: 81–89, ripening of fruit and seed) (Fig. S1). Pesticides were sprayed individually on plants at single (for dissipation kinetics) and at double dose as recommended (for processing treatments) (Polish Ministry of Agriculture web site ) by a specialized operator using knapsack sprayer to ensure sufficient pesticide primary deposit for the following processing. The plants were separated by foil. The temperature in the greenhouse ranged from 14 to 29 °C and humidity ranged from 75 to 100 % from the day of spraying until harvest.

The fungicides used included azoxystrobin (98.0%, Syngenta Biotechnology, Shanghai, China), salicylhydroxamic acid (SHAM) (99%, Shanghai Marklin Biochemistry, Shanghai, China), boscalid (99%, BASF Corporation, Nanjing, China), fluazinam (98.4%, Ishihara Sangyo Kaisha Ltd., Osaka, Japan), fludioxonil (97.9%, Syngenta Biotechnology, Co., Ltd., Shanghai, China), difenoconazole (98.4%, Yulong Chemical Industrial Co., Ltd., Hangzhou, China), pyrimethanil (93%, Jiangsu Limin Chemical Industrial Co., Ltd., Xinyi, China), and procymidone (98.5% active ingredient, Jiangxi Heyi Chemicals Company Ltd., Jiujiang, China), and were dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution (1 × 10⁵ µg/mL) and kept in darkness at −20 °C.