Simplicity 185 water system

Tetrachloroauric acid (HAuCl₄·3H₂O) was purchased from Aurat PAO (Moscow, Russia). Tris and glycine were from Amresco Chemicals (Zottegem, Belgium). γ[³²P]-ATP and T4 polynucleotide kinase (EC 2.7.1.78) were from Biosan (Novosibirsk, Russia). Fluorescein 5(6) and rhodamine B (mixed isomers) isothiocyanates and sodium citrate dihydrate (Na₃C₆H₅O₇∙2H₂O) were purchased from Sigma-Aldrich (Darmstadt, Germany). Fluorescein phosphoramidite (6-isomer), FAM CPG (6-isomer), alkyne CPG modifier, TFA-amino modifier CPG, Cyanine3 NHS ester, Cyanine5 NHS ester, Cyanine5.5 NHS ester, Cyanine7 NHS ester, Cyanine7.5 NHS ester, pyrenebutyric acid NHS ester and perylene azide were from Lumiprobe (Moscow, Russia), dodecyl amine phosphoramidite was from NooGen (Novosibirsk, Russia). All fluorescent dyes (FDs) are presented in Table S1.
Water was purified by a Simplicity 185 water system Millipore (Burlington, MA, USA) and had a resistivity of 18.2 MΩ·cm at 25 °C. The deionized water was used to prepare buffers and oligonucleotide solutions.

RNA phosphoramidites for the oligoribonucleotide synthesis were obtained from Sigma-Aldrich (Hamburg, Germany), Cy-5.5 phosphoramidite were purchased from Primetech (Minsk, Belarus). Oleylamine, dodecylamine and stearic acid were supplied from Acros Organics (Geel, Belgium). Dithiothreitol (DTT), cyanuric chloride, diethanolamine, and sodium citrate dihydrate (Na₃C₆H₅O₇) were obtained from Sigma-Aldrich (Hamburg, Germany). N,N-diisopropylethylamine (DIPEA) and N-hydroxysuccinimide (NHS) were purchased from Fluka (Seelze, Deutschland). Tetrachloroauric acid (HAuCl₄·3H₂O) was from Aurat (Moscow, Russia). Uranyl acetate (UA) and phosphotungstic acid (PTA) were purchased from SPI (West Chester, PA, USA). Peptide NH₂-(RL)₄G-C(O)NH₂·5CF₃COOH was obtained from Diapharm (Lyubertsy, Russia). Other chemicals and solvents were supplied by Merck (Darmstadt, Germany), Fluka (Seelze, Deutschland) and Panreac (Barcelona, Spain). Water was purified by a Simplicity 185 water system (Millipore, Burlington, MA, USA) and had a resistivity of 18.2 MΩ∙cm at 25 °C.

Acrylamide (AppliChem GmbH, Darmstadt, Germany); bis-Acrylamide (Amresco, Solon, OH, USA); HSA (fraction V; Renal, Budapest, Hungary); Nile Red (9-diethylamino-5H-benzo[alpha]phenoxazine-5-one (C₂₀H₁₈N₂O₂)) (Merck, Darmstadt, Germany); 1 M MgCl₂ (M1028-1ML) (Sigma, St. Louis, MO, USA); NaCl, (NH₄)₂S₂O₈ (Acros Organics, Waltham, MA, USA); N,N,N′,N′-tetramethyl ethylenediamine (TEMED) (Bio-Rad Laboratories, Berkeley, CA, USA); Tris (Fisher Scientific, Pittsburgh, PA, USA); AcOH (ice-cold) and Stains-All (Acros Organics, Waltham, MA, USA); xylene cyanol (Sigma, St. Louis, MO, USA); Ficoll 400 (Pharmacia, Stockholm, Sweden); DMEM; DMEM F12; Fetal Bovine Serum (FBS); GlutaMAX Supplement; Antibiotic-Antimycotic; and TrypLE (Gibco, Waltham, MA, USA) were used in this work. The isolation and purification of the HSA monomeric fraction were performed according to [68 (link)]. HSA modified by sulfo-Cy5 dye (HSA-Cy5) was obtained according to [69 (link)]. For the solutions, Milli-Q (18.2 M × Om/cm) water (purified by Simplicity 185 water system (Millipore, St. Louis, MO, USA)) was used.