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19 protocols using hydrogen peroxide

1

Preparation of Amyloid-beta Peptide

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1–42 peptide was synthesized by Science Peptide Biological Technology Co., Ltd. (Shanghai, China). Graphite powder (size less than 30 µm) and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) were purchased from Sinopharm Chemical Reagent Co., Ltd. Concentrated sulfuric acid, sodium nitrate, potassium permanganate, hydrochloric acid, hydrogen peroxide, sodium hydroxide and sodium carbonate were purchased from Beijing Chemical Plant. MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) was bought from Solarbio Science & Technology Co., Ltd. (Beijing, China). Deionized water used in all experiments was obtained from Milli-Q Integral 3 (Merck Millipore, France).
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2

Synthesis of Magnetic Nanocomposites

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Sulfuric acid (H2SO4, 99%; Beijing Chemical Works, China),
hydrochloric acid (HCl, ≥37%; Beijing Chemical Works, China), potassium
permanganate (KMnO4, ≥99%; Beijing Chemical Works, China), hydrogen
peroxide (H2O2, 30%; Beijing Chemical Works, China),
sodium nitrate (NaNO3, ≥99%; Sinopharm Chemical Reagent Co. Ltd.,
China), citric acid monohydrate
(C6H8O7·H2O, ≥99.5%; Sinopharm
Chemical Reagent Co. Ltd., China), natural powder graphite (325 mesh, 99.99%;
Alfa Aesar) and magnetite (Fe3O4; Xiya Reagent) were
acquired. All reagents were analytical grade and used without purification.
Water was purified using ultrapure water equipment.
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3

Synthesis of Graphene Oxide Magnetic Nanocomposites

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Graphite (> 100 mesh, 99.95%) and ammonium persulphate ((NH4)2S2O8, 99%) were both purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd., China. Ferroferric oxide (Fe3O4, 99.5%) was provided by Shanghai Macklin Biochemical Co., Ltd., China. Pyromellitic dianhydride (PMDA, 99%) and 4, 4′-oxydianiline (ODA, 99%) were both supplied by Beijing J&K Scientific Co., Ltd., China. Dimethylacetamide (DMAc, 99%) was purchased from Guangdong Guanghua Technique Co., Ltd., China. Concentrated sulfuric acid (H2SO4, 95–98%), potassium permanganate (KMnO4) and hydrogen peroxide (H2O2, 30%) were all supplied by Beijing Chemical Works, China. All materials were used without further purification.
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4

Lignin-Based Biocomposite Synthesis

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SPI (>95% protein content) was provided by Hebei Baiwei Biological Technology Co., Ltd. (Hebei, China). The enzymatic hydrolysis lignin (EHL) was supplied by Jinzhou Lingyu Chemical Co., Ltd. (Liaoning, China). ESO and sodium borohydride (NaBH4, 98%) was provided by Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Liaoning, China). Iron(ii) chloride tetrahydrate (FeCl2·4H2O) was provided by Beijing Kangpu Huiwei Technology Co., Ltd. (Beijing, China). Hydrogen peroxide (30.0%) and sodium hydroxide (NaOH) were provided by Beijing Chemical Works (Beijing, China). Ammonia solution (25% aq.) was obtained from Tianjin Huadong Reagent Factory (Tianjin, China). Anhydrous methanol (CH3OH) was provided by Beijing Huateng Chemical Co., Ltd. (Beijing, China). Poplar wood veneer (300 mm × 300 mm × 1.7 mm, 10–12% of moisture content) was provided by Wen'an (Hebei, China).
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5

Nitrile Rubber Modification Protocol

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Nitrile rubber (NANCAR1965) was obtained from Taiwan NANTEX with nitrile content in 19%; anhydrous ethanol and xylene were analytically pure; acetic acid (99%) and hydrogen peroxide (30% w/w) used were commercial grade, purchased from Beijing Chemical Plant; propionic anhydride, valeric anhydride and pentanoic anhydride were purchased from TGI; triphenylphosphine and deuterated chloroform were obtained from National Chemical Reagent Co.; Wilkinson catalyst was made in our laboratory.
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6

P2Y Receptor Expression in Cell Lines

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ATP and UTP were purchased from Sigma (St. Louis, MO, USA) and dissolved in ddH2O to a concentration of 100 mM. The antibodies of P2Y1 (H-120), P2Y2 (H-70), P2Y4 (H-60), P2Y6 (H-70), Snail (H-130), E-cadherin (G-10) and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Absolute ethyl alcohol, dimethyl benzene, formaldehyde and hydrogen peroxide were purchased from Beijing Chemical Works (Beijing, China).
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7

Siderite-Driven Oxidative Degradation of TCE

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All chemicals used in this study were prepared using ultrapure (filtered, distilled) water (Millipore Model Milli-Q Academic A10). Siderite was purchased from the Wuhan Iron and Steel (Group) Corporation, China. Hydrogen peroxide (H2O2, ~30% solution), trichloroethene (TCE, >99% purity), sodium persulfate (Na2S2O8, >98%), benzoic acid (>99.5%), p-hydroxybenzonic acid (>99.5%), and isopropanol (>99.7%) were obtained from the Beijing Chemical Plant. 5,5-Dimethyl-1-pyrroline N-oxide (DMPO, ≥97%) was purchased from Sigma-Aldrich, Inc.
The following reagent concentrations were used for all of the experiments. For TCE, 10 μL of pure TCE was added to each vial for the relevant experiments. This quantity is equivalent to 11.15 mmol/L (1,500 mg/L) in solution. This equivalent concentration exceeds the aqueous solubility of TCE, reflecting the presence of TCE liquid. Na2S2O8: 6.3 mmol/L (1,500 mg/L), H2O2: 0.15 mol/L (5,100 mg/L), siderite: 11,450 mg/L. These oxidant concentrations are relatively low compared to typical concentrations used for field applications. Thus, these concentrations will produce conservative results.
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8

Siderite Oxidation of Chlorinated Solvents

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All chemical stock solutions used in this study were prepared using ultrapure (filtered, distilled, deionized) water from a Millipore system (Millipore Model Milli-Q Academic A10). Siderite was obtained from the Wuhan Iron and Steel (Group) Corporation, China. Hydrogen peroxide (H2O2, ~30 wt. % in water), sodium persulfate (Na2S2O8, >98%), trichloroethene (TCE, >99% purity), sodium phosphate monobasic (NaH2PO4, ≥99% purity), 1,2-dichloroethane (DCA, ~99.8% purity) were purchased from Beijing Chemical Works.
The following reagent concentrations were used for all of the experiments. Oxidant concentrations were selected based on the results of previous studies employing persulfate and H2O2 (Yan et al. 2013 , 2015 (link)). For TCE, 10 µL of pure TCE was added to each vial for the relevant experiments. This quantity is equivalent to 11.15 mM (1,500 mg/L) in solution. This equivalent concentration exceeds the aqueous solubility of TCE, reflecting the presence of TCE liquid. DCA: 5 mM (495 mg/L), Na2S2O8: 6.3 mM (1,500 mg/L), H2O2: 150 mM (5,100 mg/L), siderite: 11,450 mg/L. These oxidant concentrations are relatively low compared to typical concentrations used for field applications. Thus, these concentrations will produce conservative results.
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9

Synthesis of Cerium Oxide Nanocomposites

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Cerium nitrate hexahydrate (Ce(NO3)3·6H2O, ≥99.95%) and commercial ceria (CeO2, 99.5%) were obtained from Aladdin Industrial Corporation. Tetraethoxysilane (TEOS, AP), hexamethylenetetramine (HMT, ≥99%), N-hexadecane (HD, ≥98%), sodium dodecyl sulfate (SDS, CP), and acrylic acid (AA) were bought from Xilong Chemical Co., Ltd (Guangzhou, China). Sodium bicarbonate (≥99.5%), ammonia (25 wt%), hydrogen peroxide (≥30%), and absolute ethanol were obtained from Beijing Chemical Works. 3-Methacryloxypropyltrimethoxysilane (MPS) and dodecafluoroheptyl methacrylate (DFMA) were purchased from Nanjing Xiangqian Chemical Co., Ltd and Harbin Xeogia Fluorine-Silicon Chemical Co., Ltd, respectively. All the above reagents were used as received.
Methyl methacrylate (MMA) and butyl acrylate (BA) were used as monomers which were purified using a neutral alumina column to eliminate inhibitors before use. Ammonium persulfate (APS) was obtained from Tianjin Bodi Chemical Co., Ltd, and was recrystallized before use. SiO2 with a mean diameter of 310 nm was synthesized in our lab using the Störber method.32 (link) Deionized water and absolute ethanol were used in all processes.
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10

Modification of Balsa Wood Flour

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Balsa wood
flour was supplied by Zhuhai DE CHI Technology Co., Ltd. (Zhuhai,
China). All chemicals were used without further purification. Sodium
sulfite (Na2SO3, 98%), acryloyl chloride (AC)
(C3H3ClO, 98%), and tetraethylenepentamine (TEPA)
(C8H23N5, TP) were purchased from
Heowns Biochemical Technologies Co., Ltd. (Tianjin, China). Sodium
hydroxide (NaOH, 96%, AR), ethanol (CH3CH2OH,
99.7%, AR), hydrochloric acid (HCl, 37%, AR), dichloromethane (DCM)
(CH2Cl2, 99.5%, AR), and hydrogen peroxide (H2O2, AR) were obtained from Beijing Chemical Works
(Beijing, China). Lead dinitrate (Pb(NO3)2,
99%, AR) was purchased from Shanghai Macklin Biochemical Co., Ltd.
(Shanghai, China). Zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99%, AR) was provided by Xilong Chemical
Co., Ltd. (Shantou, China), and copper nitrate trihydrate (Cu(NO3)2·3H2O, 99%, AR) was purchased
from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai,
China).
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