Sc30 camera

Any morphological changes of the isolated lung CSCs post-PDT treatment were observed and studied using an Olympus CKX41 inverted light microscope (Wirsam, Olympus CKX41) 24 h post-irradiation, whereby changes were captured using the SC30 Olympus camera.

The cells were routinely cultured at 37 °C in a humidified atmosphere containing air and 5% CO₂. Cytotoxicity of colloid solutions of polymer nanoparticles with concentrations 0.05–0.5 mg/mL was evaluated with a standard MTT test as described elsewhere [33 (link)]. The incubation of nanoparticles with cells was performed for 48 h. The values measured at 540 nm were subtracted for background correction at 690 nm, and the data was plotted as a percent of control samples using Microsoft Excel software (Microsoft Corp., Redmond, WA, USA).
To monitor if the particles penetrate the cells, 200 µL of cell culture medium containing Caco-2 cells were seeded on glass chamber slides (LabTec-II with CC2 treatment) and cultured for 24 h. Then, the incubation medium was changed with fresh one containing rhodamine-loaded polymersomes and the mixtures were incubated for 4 h. After that, the cells were washed three times with warm PBS solution and observed using a fluorescence microscope (Olympus IX50, Olympus Corp., Tokyo, Japan) equipped with a SC30 Olympus camera to capture the images of cells (excitation filter: BP 530–550, barrier filter: BA590). The images were acquired at 20× optical zoom.

Small pieces of kidney were extracted from each specimen and fixed immediately in 5% cold buffered glutaraldehyde for 24–48 h, cleaned in cacodylate buffer (pH 7.2) 3–4 times for 20 min, postfixed in 1% osmium tetroxide for 2 h, rewashed in the same buffer 4 times, and then dehydrated in alcohol. The specimens were fixed in Epon–Araldite mixture. From the embedded blocks, the semithin sections with thickness (0.5–1 µm) were cut by LKB ultramicrotome (Bromma, Sweden) stained with toluidine blue and photographed by sc30 Olympus camera (Münster, Germany). The ultrathin Sect. (500–700 A◦ thickness) was cut using Leica AG ultramicrotome (Wetzlar, Germany) and stained with uranyl acetate and lead citrate. The stained sections were then examined using the JEM-100CXII operating at 80 kV and photographed by CCD digital camera Model XR-41 (CA, USA).