Human mpo elisa kit

Manufactured by Hycult Biotech

Sourced in United States

The Human MPO ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of myeloperoxidase (MPO) levels in human samples. The kit employs the quantitative sandwich enzyme immunoassay technique and utilizes a pre-coated microplate with a specific antibody for MPO.

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Spelling variants of this product

MPO ELISA kit (12 citations) MPO kit (2 citations) ELISAs (2 citations)

3 protocols using human mpo elisa kit

Quantification of NET-associated MPO/DNA complexes

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MPO is present on extruded NETs. To detect such structures, NET-associated MPO/DNA complexes were quantified by using a modified capture ELISA
[21 (link)]. In brief, NET-associated MPO in serum or culture supernatant was captured by using the coated 96-well plate of the human MPO ELISA Kit, (Hycult Biotech), after which the NET-associated DNA backbone was detected by using the detection antibody of the Human Cell Death Detection ELISA^PLUS (Roche Diagnostics).

Sur Chowdhury C., Giaglis S., Walker U.A., Buser A., Hahn S, & Hasler P. (2014). Enhanced neutrophil extracellular trap generation in rheumatoid arthritis: analysis of underlying signal transduction pathways and potential diagnostic utility. Arthritis Research & Therapy, 16(3), R122.

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Quantification of NET-associated Biomarkers

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The concentrations of NE and MPO were measured in sera and plasma by sandwich ELISA, utilizing, respectively, the Elastase/a1-PI Complex ELISA Kit (Calbiochem) and the human MPO ELISA Kit. Histone/DNA complexes in sera and plasma were measured using the Human Cell Death Detection ELISA^PLUS (Roche Diagnostics); nucleosomes in cell culture supernatants were detected similarly after incubation with DNase I (10 U for 5 min) (Roche Diagnostics). To identify NET-associated MPO/DNA complexes, a modified capture ELISA was utilized (27 (link)). NET-associated MPO in culture supernatant was captured using the coated 96-well plate of the human MPO ELISA Kit (Hycult Biotech), and the NET-associated DNA backbone was detected using the anti-DNA-POD antibody of the Human Cell Death Detection ELISA^PLUS (Roche Diagnostics). G-CSF serum and plasma protein concentrations were assessed with the Human G-CSF Quantikine ELISA Kit (R&D Systems).

Giaglis S., Stoikou M., Sur Chowdhury C., Schaefer G., Grimolizzi F., Rossi S.W., Hoesli I.M., Lapaire O., Hasler P, & Hahn S. (2016). Multimodal Regulation of NET Formation in Pregnancy: Progesterone Antagonizes the Pro-NETotic Effect of Estrogen and G-CSF. Frontiers in Immunology, 7, 565.

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Quantifying NET-associated biomarkers in sepsis

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Blood samples ( plasma and serum) from each patient were collected upon emergency department admission and on days 3, 5 and 7, and processed as previously described [9] .
Cell-free nucleosomes as surrogate of NETosis were measured in serum samples of the total study population using Human Cell Death Detection ELISA PLUS (Roche Diagnostics, Basel, Switzerland). The concentrations of neutrophil elastase and MPO were measured in sera and plasma by sandwich ELISA, utilising the Elastase/α1-PI complex ELISA kit (Calbiochem/EMD, Gibbstown, NJ, USA) and the human MPO ELISA kit (Hycult Biotech, Plymouth Meeting, PA, USA), respectively. To specifically characterise the source of cell-free nucleosomes as NET-associated MPO-DNA complexes, MPO-specific capture and subsequent DNA-specific detection antibodies were used as previously described [12] . For detailed methodology, see supplementary material [13] [14] (link)[15] [16] [17] [18] [19] [20] .

Ebrahimi F., Giaglis S., Hahn S., Blum C.A., Baumgartner C., Kutz A., van Breda S.V., Mueller B., Schuetz P., Christ-Crain M, & Hasler P. (2018). Markers of neutrophil extracellular traps predict adverse outcome in community-acquired pneumonia: secondary analysis of a randomised controlled trial. The European respiratory journal, 51(4).

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