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Multiscreen ha mixed cellulose ester membrane plates

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Multiscreen HA mixed cellulose ester membrane plates are a type of lab equipment used for filtration and separation processes. The plates contain a mixed cellulose ester membrane that can be used for various applications, such as cell culture, protein purification, and sample preparation.

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Lab products found in correlation

Alkaline phosphatase conjugated goat anti mouse total ig secondary ab, by Southern Biotech (5 mentions) Bcip nbt substrate, by Merck Group (3 mentions) Nbt bcip substrate nitroblue tetrazolium 5 bromo 4 chloro 3 indolyl phosphate, by Merck Group (2 mentions) 5 bromo 4 chloro 3 indolyl phosphate nitro blue tetrazolium, by Merck Group (1 mentions)

Close spelling variants of this product

MultiScreen HA mixed cellulose ester plates Mixed cellulose esters Mixed cellulose membranes MultiScreen-HA plates Cellulose ester membrane MultiScreen-HA Multiscreen plates Cellulose membrane

5 protocols using multiscreen ha mixed cellulose ester membrane plates

1

Quantifying Anti-GPI Antibody Secreting Cells

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Anti-GPI antibody secreting cells were measured by ELISpot as described (25 (link)). Briefly, cells from the joint draining lymph nodes (axillary, brachial, and popliteal LNs) from 6 week-old KRN.g7, IDO2 ko KRN.g7, R235W KRN.g7, and Y346X KRN.g7 mice were plated at 4 × 105 cells per well and diluted serially 1:4 in Multiscreen HA mixed cellulose ester membrane plates (Millipore) coated with histidine (his)-tagged glucose-6-phosphate isomerase (GPI) (10μg/ml). The cells were incubated on the GPI-coated plates for 4hr at 37°C. The Ig secreted by the plated cells was detected by Alkaline Phosphatase-conjugated goat anti-mouse total Ig secondary Ab (Southern Biotechnology Associates) and visualized using NBT/BCIP substrate (nitroblue tetrazolium / 5-bromo-4-chloro-3-indolyl phosphate; Sigma).
Merlo L.M., Peng W., DuHadaway J.B., Montgomery J.D., Prendergast G.C., Muller A.J, & Mandik-Nayak L. (2021). The Immunomodulatory Enzyme IDO2 Mediates Autoimmune Arthritis Through a Non-Enzymatic Mechanism. Journal of immunology (Baltimore, Md. : 1950), 208(3), 571-581.
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2

Quantifying Anti-GPI Antibody Secreting Cells by ELISpot

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Anti-GPI antibody secreting cells were measured by ELISpot as described (36 (link)). Briefly, cells from the joint draining lymph nodes (axillary, brachial, and popliteal LNs) from 6 week-old KRN.g7, IDO1 ko KRN.g7, IDO2 ko KRN.g7, and dko KRN.g7 mice were plated at 4 × 105 cells per well and diluted serially 1:4 in Multiscreen HA mixed cellulose ester membrane plates (Millipore) coated with GPI-his (10 μg/ml). The cells were incubated on the Ag-coated plates for 4 h at 37°C. The Ig secreted by the plated cells was detected by Alkaline Phosphatase-conjugated goat anti-mouse total Ig secondary Ab (Southern Biotechnology Associates) and visualized using NBT/BCIP substrate (nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate; Sigma).
Merlo L.M., DuHadaway J.B., Montgomery J.D., Peng W.D., Murray P.J., Prendergast G.C., Caton A.J., Muller A.J, & Mandik-Nayak L. (2020). Differential Roles of IDO1 and IDO2 in T and B Cell Inflammatory Immune Responses. Frontiers in Immunology, 11, 1861.
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3

ELISPOT Assay for Ig-Secreting Cells

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Cells from the joint draining LN (axillary, brachial, and popliteal LNs) from 6 week-old control mouse Ig or IDO2 Ig-treated KRN.g7 mice were plated at 4 × 105 cells per well and diluted serially 1:4 in Multiscreen HA mixed cellulose ester membrane plates (Millipore) coated with GPI-his (10μg/ml). The cells were incubated on the Ag-coated plates for 4hr at 37ºC. The Ig secreted by the plated cells was detected by Alkaline Phosphatase-conjugated goat anti-mouse total Ig secondary Ab (Southern Biotechnology Associates) and visualized using NBT/BCIP substrate (nitroblue tetrazolium / 5-bromo-4-chloro-3-indolyl phosphate; Sigma).
Merlo L.M., Grabler S., DuHadaway J.B., Pigott E., Manley K., Prendergast G.C., Laury-Kleintop L.D, & Mandik-Nayak L. (2017). Therapeutic Antibody Targeting of Indoleamine-2,3-Dioxygenase (IDO2) Inhibits Autoimmune Arthritis. Clinical immunology (Orlando, Fla.), 179, 8-16.
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4

Quantifying Antigen-specific B Cells

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Cells from the joint draining LN (axillary, brachial, and popliteal LNs) from 6 week-old KRN.g7, IDO1 ko KRN.g7, and IDO2 ko KRN.g7 mice were plated at 4 × 105 cells per well and diluted serially 1:4 in Multiscreen HA mixed cellulose ester membrane plates (Millipore) coated with GPI-his (10μg/ml). The cells were incubated on the Ag-coated plates for 4hr at 37°C. The Ig secreted by the plated cells was detected by Alkaline Phosphatase-conjugated goat anti-mouse total Ig secondary Ab (Southern Biotechnology Associates) and visualized using NBT/BCIP substrate (nitroblue tetrazolium / 5-bromo-4-chloro-3-indolyl phosphate; Sigma).
Merlo L.M., Pigott E., DuHadaway J.B., Grabler S., Metz R., Prendergast G.C, & Mandik-Nayak L. (2014). IDO2 is a critical mediator of autoantibody production and inflammatory pathogenesis in a mouse model of autoimmune arthritis. Journal of immunology (Baltimore, Md. : 1950), 192(5), 2082-2090.
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5

Quantifying Mouse Antibody-Secreting Cells

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LN cells were plated at 4 × 105 cells per well and diluted serially 1:4 in Multiscreen HA mixed cellulose ester membrane plates (Millipore) coated with GPI-his (5µg/ml). The cells were incubated on the Ag-coated plates for 4h at 37°C. The Ig secreted by the plated cells was detected by Alkaline Phosphatase-conjugated goat anti-mouse total Ig secondary Ab (Southern Biotechnology Associates) and visualized using NBT/BCIP substrate (nitroblue tetrazolium / 5-bromo-4-chloro-3-indolyl phosphate; Sigma).
Pigott E., DuHadaway J.B., Muller A.J., Gilmour S., Prendergast G.C, & Mandik-Nayak L. (2014). 1-Methyl-Tryptophan Synergizes with Methotrexate to Alleviate Arthritis in a Mouse Model of Arthritis. Autoimmunity, 47(6), 409-418.
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