Image pro insight software

Manufactured by Media Cybernetics

Sourced in United States

Image-Pro Insight software is a comprehensive image analysis tool that allows users to capture, process, measure, and analyze digital images. It provides a suite of image processing and analysis tools for a wide range of applications.

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Lab products found in correlation

Cryostat, by Leica (1 mentions) Sm2000r, by Leica (1 mentions) Vector elite abc kit, by Vector Laboratories (1 mentions) Mouse monoclonal anti neun, by Merck Group (1 mentions) Eclipse e600, by Nikon (1 mentions) Az100 multizoom macroscope, by Nikon (1 mentions) Cyber shot, by Zeiss (1 mentions) Dmlm microscope, by Leica (1 mentions) Evolution mp camera, by Media Cybernetics (1 mentions)

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Image-Pro software (285 citations) Image-Pro (129 citations) Image-Pro Insight (10 citations)

8 protocols using image pro insight software

Hippocampal Cytoarchitecture Analysis in Rat Brains

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Rat brains were cryopreserved with 18% sucrose and sectioned into 20 μm slices using a Leica cryostat. Coronal tissue sections containing exemplary dorsal hippocampus, analogous to Plate 31 of Paxinos and Watson, 1998, were collected and stained using cresyl violet.
We conducted a qualitative morphology description of the CA1 and CA3 subregions of the hippocampus and performed a cell count in photomicrographs at 10x magnification with the aid of the manual marker in Image-Pro Insight software (Media Cybernetics, Inc., Rockville, MD, USA). Cell counting was made in 3 different fields of 0.2 mm × 0.2 mm on each of 5 representative sections through the dorsal hippocampus. Counting was made by 4 different blinded subjects following these criteria: neurons with a visible nucleus and/or a complete cell contour.
We also used a scale to classify the cell dispersion of CA1, CA2, CA3, and GG. The sections were scored by 4 different blinded subjects using a semiquantitative grading system (see Table 1) as described by Shafri et al. [36 ].

Verdugo-Diaz L., Estrada-Rojo F., Garcia-Espinoza A., Hernandez-Lopez E., Hernandez-Chavez A., Guzman-Uribe C., Martinez-Vargas M., Perez-Arredondo A., Calvario T., Elias-Viñas D, & Navarro L. (2017). Effect of Intermediate-Frequency Repetitive Transcranial Magnetic Stimulation on Recovery following Traumatic Brain Injury in Rats. BioMed Research International, 2017, 4540291.

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Immunohistochemical Thalamic Lesion Analysis

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Half of the brain was immediately placed in 4% paraformaldehyde followed by sucrose cyroprotection and sectioned sagittally at 50 μM using a sliding microtome (SM 2000R; Leica Instruments, Wetzlar, Germany). To visualize thalamic lesions, neuronal nuclei were labeled using a primary antibody for NeuN. Washes were performed between each step (3X, 5-min each in Tris Buffered Saline, TBS). Slices were incubated for 30 min in 3% hydrogen peroxide for 30-min in a solution of 10% normal horse serum and 1% Triton X-100 in TBS and 48-hrs in monoclonal mouse anti-NeuN (1:500, Millipore, Temecula, CA) at 4°C. After rinses, slices were incubated for 2-hrs in biotinylated horse anti-mouse IgG (Vector Lab Inc, Burlington, CA) and then 2-hrs in an avidin-biotin conjugate solution (Vector ABC Elite kit; Vector Laboratories, Inc.). Finally, sections were exposed to 3,3′-diaminobenzidine for 2-min for sufficient chromogenic signal. Sections approximately 0.05, 1.0, and 2.0 mm lateral from the midline (Paxinos and Watson, 2013 ) were imaged at 4X on a Nikon microscope (Eclipse E400, Japan) using Image-Pro Insight software (MediaCybernetics, Rockville, MD). Extent of thalamic neuronal was assessed using Image J (NIH, freeware).

Vedder L.C., Hall J.M., Jabrouin K.R, & Savage L.M. (2015). Interactions between chronic ethanol consumption and thiamine deficiency on neural plasticity, spatial memory and cognitive flexibility. Alcoholism, clinical and experimental research, 39(11), 2143-2153.

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Morphological Assessment of L. acidophilus

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Morphological assessment of L. acidophilus NCFM strain was performed using a phase-contrast light microscope at ×40 magnification (Nikon Eclipse E600). Cells were observed over a growth period of 24 h in MRS broth at 37°C or MRS broth with 5 μg/ml erythromycin for the complemented strain NCK2397. Pictures were taken using a QImaging MicroPublisher 5.0 RTV camera attachment at 1-, 4-, 7-, 14-, and 24-h time points. Cell chain length was measured using Image-Pro Insight software (Media Cybernetics).

Johnson B.R, & Klaenhammer T.R. (2016). AcmB Is an S-Layer-Associated β-N-Acetylglucosaminidase and Functional Autolysin in Lactobacillus acidophilus NCFM. Applied and Environmental Microbiology, 82(18), 5687-5697.

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Leaf Anatomy and Morphology Analysis

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Plants were photographed 32 days after planting, and fully expanded leaf 5 (i.e. the fifth leaf to emerge after germination) was removed at the ligule for phenotypic analysis. Leaf length, width and midrib extension (the point along the proximal/distal axis at which the midrib was no longer visible) were recorded. Segments of leaf encompassing the midrib and the three or four adjacent lateral veins were cut from the midpoint along the proximal/distal axis and positioned upright in 7% agar. Once cooled, blocks were trimmed and mounted such that veins were vertically orientated. Sections (50-60 μm) were cut using a vibratome and then cleared for around 10 min in 3:1 ethanol:acetic acid. Sections were incubated in 70% ethanol overnight, then floated on slides with 70% ethanol (v/v) and covered with a coverslip. Leaf sections were imaged using a Leica DMRB microscope with QImaging MicroPublisher camera (QImaging, www.qimaging.com) and Image-Pro Insight software (MediaCybernetics, www.mediacy.com). Images were taken using bright-field (which enabled BS and M cells to be identified) and UV (which enabled sclerenchyma and thus vein orders to be determined) illumination. Images were tiled together so that the region of leaf between two lateral veins was represented. Subsequent quantification of segment width was undertaken using the ImageJ software package (https://imagej.nih.gov/).

Hughes T.E., Sedelnikova O.V., Wu H., Becraft P.W, & Langdale J.A. (2019). Redundant SCARECROW genes pattern distinct cell layers in roots and leaves of maize. Development (Cambridge, England), 146(14), dev177543.

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Analysis of Plant Trichome Birefringence

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Trichomes were analyzed from the first two leaves of 14-d-old plants. Three biological replicates were performed between the months of September and October. Leaves were processed for microscopy using a modified protocol based on Sharma (43 ) as follows: Leaves were cleared for 3 d in a 7:1 solution of 95% ethanol acetic acid, washed for 20 min in 1 N KOH, and rinsed once in water. Leaves were mounted between a microscope slide and coverslip in water for immediate analysis or, for semipermanent samples, in 65% glycerol. Trichome birefringence was captured placing the sample between two polarizer lenses (aligned perpendicularly respective to each other) and magnified with an AZ100 Multizoom Macroscope (Nikon Instruments Inc.) using a Plan Fluor 2x objective (N.A. 0.2) plus an internal 2

\times

magnification. Lateral LED illumination was used simultaneously to photograph the leaf surface. XY series of images were assembled to form images of the whole leaf using Image Pro Insight software (Media Cybernetics). The position of each trichome base was marked using Adobe Photoshop CS6, and images were segmented.

Di Patti F., Ugartechea Chirino Y., Arbel-Goren R., Sharon T., Castillo A., Alvarez–Buylla E., Fanelli D, & Stavans J. (2023). Stochastic Turing patterns of trichomes in Arabidopsis leaves. Proceedings of the National Academy of Sciences of the United States of America, 120(42), e2309616120.

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Visualizing and Comparing Growth Profiles of Lactobacillus acidophilus Derivatives

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Cultures were propagated from frozen stocks and subcultured twice at a 1% (vol/vol) inoculum in MRS under standard conditions and harvested after 16 h incubation (stationary phase). Cell morphology and chain length was visualized using a Nikon Eclipse E600 phase contrast microscope with a Q-Imaging Micropublisher Camera attachment. At least 40 cells from each of three biological replicates were measured using Image Pro Insight software (Media Cybernetics, Inc., Rockville, MD, USA). Chain lengths were averaged and means were compared using a two-tailed t-test with unequal variance, at a significance threshold of p < 0.05.
For comparing the growth profiles of each L. acidophilus derivative, cultures were propagated from frozen stocks in MRS and subcultured twice at a 1% (vol/vol) inoculum in GSDM under standard conditions. The growth of each strain in both standard and high Mn medium was monitored in triplicate using a Spec-20 spectrophotometer at 600 nm and reported as an average.

Selle K., Goh Y.J., Johnson B.R., O’Flaherty S., Andersen J.M., Barrangou R, & Klaenhammer T.R. (2017). Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus. Frontiers in Microbiology, 8, 553.

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Quantifying Uterine NK Cells in RIF

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Immunohistochemistry (IHC) with pre-diluted anti-CD56 (clone 123C3) mouse monoclonal primary antibody (Ventana Medical Systems, Roche Diagnostics, Germany) was performed on twenty endometrial biopsies obtained from ten RIF patients to detect uterine NK cells (uNKs). CD56-positive cells were counted per one mm
²of the tissue slide. Images were taken and prepared with an on-board camera (Cyber-Shot, Zeiss, Japan). Scale bars were produced using a morphometric lens (CH-2, Olympus, Germany) and proofed with Meazure software (C Thing Software, Sunnyvale, CA, USA). All images were captured under 100 × magnification. Pixel-based frequency values were obtained using Image-Pro-insight software (Version 9.0, Media Cybernetics, USA).

Bahrami-Asl Z., Farzadi L., Fattahi A., Yousefi M., Quinonero A., Hakimi P., Latifi Z., Nejabati H.R., Ghasemnejad T., Sadigh A.R., Heidari M.H., Nouri M., Novin M.G, & Dominguez F. (2020). Tacrolimus Improves the Implantation Rate in Patients with Elevated Th1/2 Helper Cell Ratio and Repeated Implantation Failure (RIF). Geburtshilfe und Frauenheilkunde, 80(8), 851-862.

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Leica DM LM Microscope Imaging

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Light microscopy was conducted on a Leica DM LM microscope (Leica Microsystems GmbH, Wetzlar, Germany) equipped with an Evolution MP Camera (Media Cybernetics, Rockville, MD, USA) controlled by Image-Pro Insight software (Media Cybernetics, Rockville, MD, USA). The microscope was operated in both, transmitted-light mode and cross polarized-light mode at 100× magnification.

Liu J., Hwu E., Bannow J., Grohganz H, & Rades T. (2022). Impact of Molecular Surface Diffusion on the Physical Stability of Co-Amorphous Systems. Molecular pharmaceutics, 19(4).

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