Dve00

Manufactured by R&D Systems

Sourced in United States

The DVE00 is a piece of laboratory equipment designed for general use in research and development applications. It serves as a versatile tool for conducting various experiments and analyses within a controlled laboratory environment.

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Lab products found in correlation

Db100b, by R&D Systems (4 mentions) Vascular endothelial growth factor (vegf), by R&D Systems (2 mentions) Dg100, by R&D Systems (2 mentions) D6050, by R&D Systems (2 mentions) D1500, by R&D Systems (1 mentions) Dif50, by R&D Systems (1 mentions) Celltiter glo 2.0 cell viability assay, by Promega (1 mentions) Vegf sc 7269, by Santa Cruz Biotechnology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Dcp00, by R&D Systems (1 mentions) Dmem f12, by Cayman Chemical (1 mentions) 6 well plate, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Merck Group (1 mentions) Af810, by R&D Systems (1 mentions) Hdmec, by ScienCell (1 mentions) Transwell apparatus, by Corning (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Dgb100, by R&D Systems (1 mentions) Spectramax 190, by Molecular Devices (1 mentions)

55 protocols using dve00

Quantifying hCB-EPC Paracrine VEGF

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The paracrine function of hCB-EPCs was evaluated by assaying vascular endothelial growth factor (VEGF) concentration in the supernatant of cultured cells. On day 4 of the culture of hCB-EPCs and HUVECs, medium was changed to Dulbecco’s Modified Eagle’s Medium (DMEM) without growth factors but containing 10% FBS, and cultured continuously for 72 h. After centrifuging collected medium at 1000 × g for 10 min, the supernatant was used to detect VEGF by ELISA (DVE00, R&D Systems) according to the manufacturer’s instructions.

Sun H., Morihara R., Feng T., Bian Z., Yu H., Hu X., Hu X., Bian Y., Sasaki R., Fukui Y., Takemoto M., Yunoki T., Nakano Y., Abe K, & Yamashita T. (2023). Human Cord Blood–Endothelial Progenitor Cells Alleviate Intimal Hyperplasia of Arterial Damage in a Rat Stroke Model. Cell Transplantation, 32, 09636897231193069.

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Quantification of VEGF-A in Cell Culture

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VEGF-A levels in cell culture supernatants were detected using a human ELISA kit (DVE00, R&D Systems), according to the manufacturer’s instructions. Briefly, collected cell culture supernatants and standards were added in various dilutions to 96-well plates coated with capture antibody, and they were then incubated with biotinylated detection antibody. After incubation with streptavidin-peroxidase and TMB substrate, the protein contents in supernatants were determined by measuring the absorbance at 450 nm (OD₄₅₀) using a plate reader (BioTek Instruments).

Ye Q., Zhang J., Zhang C., Yi B., Kazama K., Liu W., Sun X., Liu Y, & Sun J. (2022). Endothelial PRMT5 plays a crucial role in angiogenesis after acute ischemic injury. JCI Insight, 7(9), e152481.

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Serum VEGF Quantification Protocol

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Blood samples were collected in anticoagulant-free tubes during the morning (between 08:00 and 09:00 h). Serum was then separated, sealed and stored at − 80 °C. VEGF levels were measured using a commercially available kit (DVE00; R&D Systems, Minneapolis, IN, USA) according to the manufacturer’s instructions. All tests were conducted in duplicate for each concentration. Calculation of VEGF was performed using a standard curve for recombinant human VEGF protein. The lower detection limit was 9 pg/mL. Intra- and inter-assay variances were below 5.1 and 6.2%, respectively. Sample collection and analyses were performed in a blinded manner.

Zhao Y., Xiao W., Chen K., Zhan Q., Ye F., Tang X, & Zhang X. (2019). Neurocognition and social cognition in remitted first-episode schizophrenia: correlation with VEGF serum levels. BMC Psychiatry, 19, 403.

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Measuring hVEGF-A165 Levels

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Plasma levels of hVEGF-A₁₆₅ were measured with enzyme-linked immunoassay (#DVE00; R&D Systems).

Kivelä A.M., Huusko J., Gurzeler E., Laine A., Dijkstra M.H., Dragneva G., Andersen C.B., Moestrup S.K, & Ylä-Herttuala S. (2017). High Plasma Lipid Levels Reduce Efficacy of Adenovirus-Mediated Gene Therapy. Scientific Reports, 7, 386.

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Multiplex Cytokine Profiling in CSF and Serum

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The serum and CSF samples were detected for the following 13 factors: IL‐2 (R&D, D2050), IL‐6 (R&D, D6050), IL‐10 (R&D, D1000B), IL‐15 (R&D, D1500), IL‐17 (R&D, D1700), G‐CSF (R&D, DCS50), GM‐CSF (R&D, DGM00), bFGF (R&D, DFB50), VEGF (R&D, DVE00), MIP‐1α (R&D, DMA00), MIP‐1β (R&D, DMB00), MCP‐1 (R&D, DCP00), and IFN‐γ (R&D, DIF50). Every factor was assayed using ELISA kits and conducted per the manufacturer's protocols. All the marker measurements were performed by blinded independent investigators to avoid subjective bias.

Guo J., Yang X., Gao L, & Zang D. (2017). Evaluating the levels of CSF and serum factors in ALS. Brain and Behavior, 7(3), e00637.

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VEGFA and SHH Quantification

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The on-gel culture supernatant was collected and centrifuged at 200 × g for 10 min at 4°C. The supernatant was used for ELISA, which was performed according to the kit’s instructions (R&D Systems DVE00 and DSHH00). The number of cells on the plate from which the culture supernatant was removed was counted using CellTiter-Glo 2.0 Cell Viability Assay (Promega G9241), and the amount of VEGFA and SHH was corrected using the values of CellTiter-Glo.

Ohnishi Y., Masui A., Suezawa T., Mikawa R., Hirai T., Hagiwara M, & Gotoh S. (2024). Screening of factors inducing alveolar type 1 epithelial cells using human pluripotent stem cells. Stem Cell Reports, 19(4), 529-544.

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Quantifying Serum VEGF Levels

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Serum concentration of VEGF was measured using a commercially available sandwich ELISA kit according to the manufacturer’s instruction (DVE00; R&D Systems, Minneapolis, MN, USA). The coefficient of variance for serum samples as listed on the manufacturer’s data sheet is 0.045–0.067. All analyses, including the standards, were performed in duplicates and the average intra-assay coefficient of variance for the samples was 0.049. All concentrations were above the manufacturer’s reported minimum detectable dose of 9 pg/ml.

Fukuda A.M., Hindley L.E., Kang J.W., Tirrell E., Tyrka A.R., Ayala A, & Carpenter L.L. (2020). Peripheral Vascular Endothelial Growth Factor Changes after Transcranial Magnetic Stimulation in Treatment Resistant Depression. Neuroreport, 31(16), 1121-1127.

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ELISA Quantification of PGE2 and VEGF

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PGE₂ and VEGF in culture supernatants were determined with ELISA kits (Abcam #Ab136948 and R&D Systems #DVE00 respectively), according to the manufacturer’s instructions.

Garrido M.P., Hurtado I., Valenzuela-Valderrama M., Salvatierra R., Hernández A., Vega M., Selman A., Quest A.F, & Romero C. (2019). NGF-Enhanced Vasculogenic Properties of Epithelial Ovarian Cancer Cells Is Reduced by Inhibition of the COX-2/PGE2 Signaling Axis. Cancers, 11(12), 1970.

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Quantitative VEGF Measurement in Alx3 Conditioned Media

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VEGF was quantitatively measured in vector control conditioned medium, Alx3 conditioned medium and VEGF-depleted Alx3 conditioned medium using commercial ELISA Kit (DVE00, R&D) per manufacturer protocol. Briefly, 100-μl conditioned samples were incubated in microplate stripes at R.T. for 2 hrs, followed by 2-hr incubation with 200-μl conjugate and incubation of 200 μl substrate for 30 mins, followed by measuring 450-nm absorbance. Each experiment was conducted in at least biological triplicates. Alx3 conditioned medium was incubated with VEGF (sc-7269, 1 μg per 400 μg total protein, Santa Cruz) at 4°C overnight, with normal goat IgG serum as a negative control. A total of 100-μl agarose bead slurry was incubated for 1 hr with gentle agitation to obtain supernatants.

He L., Zhou J., Chen M., Lin C.S., Kim S.G., Zhou Y., Xiang L., Xie M., Bai H., Yao H., Shi C., Coelho P.G., Bromage T.G., Hu B., Tovar N., Witek L., Wu J., Chen K., Gu W., Zheng J., Sheu T.J., Zhong J., Wen J., Niu Y., Cheng B., Gong Q., Owens D.M., Stanislauskas M., Pei J., Chotkowski G., Wang S., Yang G., Zegarelli D.J., Shi X., Finkel M., Zhang W., Li J., Cheng J., Tarnow D.P., Zhou X., Wang Z., Jiang X., Romanov A., Rowe D.W., Wang S., Ye L., Ling J, & Mao J.J. (2019). Parenchymal and Stromal Tissue Regeneration of Tooth Organ by Pivotal Signals Reinstated in Decellularized Matrix. Nature materials, 18(6), 627-637.

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ARPE-19 Cytokine Response to DP2 Agonist

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ARPE-19 cells were cultured in DMEM/F-12 (Sigma–Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (Life Technologies Corporation, Carlsbad, CA) and 1% penicillin-streptomycin solution (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in 5% CO2/95% air. The culture medium was replaced every 48 hours. In the experiments, cells were plated on 6-well plates (Nunc, Rochester, NY) at a density of 100,000 cells/well in 2 mL of serum-containing medium and incubated for 5 days. Confluent cell cultures were washed with serum-free DMEM/F-12 medium and treated with or without 15 µM CAY10471 in a serum-free medium and after 2 hours of incubation in the presence or absence of 15 µM 15(R)-15-methyl PGD₂, a stable DP2-selective agonist (Cayman Chemical, #12720). After 22 hours of incubation, the medium was collected for cytokine measurements. The concentrations of VEGF and MCP-1 were measured using ELISA kits (R&D Systems, DVE00 and DCP00, respectively) according to the manufacturer's instructions.

Soga H., Inoue T., Urade Y., Ueta T., Kawashima H., Kaburaki T, & Aihara M. (2023). Attenuation of Laser-Induced Choroidal Neovascularization by Blockade of Prostaglandin D2 Receptor 2. Translational Vision Science & Technology, 12(5), 5.

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