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Round bottom polystyrene tubes

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Round bottom polystyrene tubes are a type of laboratory equipment used for various purposes. They are made of polystyrene, a common plastic material, and feature a rounded bottom shape. These tubes are designed to hold and contain liquid samples or solutions during scientific experiments and procedures.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Freezone 1 liter benchtop freeze dry system, by Labconco (1 mentions) Galliostm flow cytometer, by Beckman Coulter (1 mentions) Dihydrorhodamine 123 dhr123, by Merck Group (1 mentions) Kaluza flow cytometry software, by Beckman Coulter (1 mentions) Pbs 1x, by Thermo Fisher Scientific (1 mentions)

3 protocols using round bottom polystyrene tubes

1

Transfection and Flow Cytometry of HEK293T Cells

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HEK293T were seeded, cultured and transfected as mentioned above. 48 h after transfection cells were trypsinized, collected and transferred to 5 ml round bottom polystyrene tubes (Corning). The cells were spun down at 900 xg for 5 min, the pellet was washed once with cold PBS and resuspended in 0.5 ml of PBS containing 1 mM EDTA and 2% FBS (Gibco, Thermo Fisher Scientific). 4ʹ,6-diamidino-2-phenylindole (DAPI) staining was added to all samples to exclude dead cells from the analysis and doublets were excluded by forward/side scatter area versus height gating. The samples were measured with MACSQuant Analyser 10 cytometer (Miltenyi Biotec) and data was analysed with FlowJo software (FlowJo, LLC, version 10.0.7).
Corso G., Heusermann W., Trojer D., Görgens A., Steib E., Voshol J., Graff A., Genoud C., Lee Y., Hean J., Nordin J.Z., Wiklander O.P., El Andaloussi S, & Meisner-Kober N. (2019). Systematic characterization of extracellular vesicle sorting domains and quantification at the single molecule – single vesicle level by fluorescence correlation spectroscopy and single particle imaging. Journal of Extracellular Vesicles, 8(1), 1663043.
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2

Lyophilization of Microbial Cultures

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Early stationary-phase cultures (600 ml) were centrifuged at 6000×g for 10 min, washed once with 100 ml of phosphate-buffered saline, pH 7.4 and recentrifuged. Each cell pellet was resuspended in 12 ml of Microbial Freeze Drying buffer (OPS Diagnostics, NJ, USA) and aliquoted into 1 ml samples in 5 ml, round-bottom, polystyrene tubes (Corning Inc., NY, USA). These samples were then snap-frozen in liquid nitrogen and lyophilized overnight using a FreeZone 1 Liter Benchtop Freeze Dry System (Labconco Inc., MO, USA). Lyophilized samples were stored at 4 °C in air and rehydrated with 1 ml of anaerobic SBO for 10 min at room temperature prior to plating for CFUs.
Ellis M.L., Dowell A.E., Li X, & Knight J. (2016). Probiotic properties of Oxalobacter formigenes: an in vitro examination. Archives of microbiology, 198(10), 1019-1026.
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3

Measuring Neutrophil and Monocyte ROS Production

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One milliliter of whole blood was used and erythrocytes were lysed in ammonium buffer. After lysis, cells were washed with PBS 1X (ThermoFisher Scientific) and resuspended in 1 mL of PBS, 200 μL of the cell suspension was transferred in 5 mL round-bottom polystyrene tubes (Corning Inc., USA) and kept at 37 °C in a 5% CO2 incubator throughout the experiment. ROS production on neutrophils and monocytes was induced by 4–40 and 400 ng/mL PMA in the presence of 150 μL of dihydrorhodamine 123 (DHR 123) at 5 mg/mL (Sigma). Twenty minutes after PMA treatment, cells were placed on ice and DHR oxidation was measured by flow cytometry using GalliosTM Flow Cytometer (Beckman Coulter, USA) Data were analyzed with Kaluza Flow Cytometry Software (version 1.2; Beckman Coulter).
Moens L., Gouwy M., Bosch B., Pastukhov O., Nieto-Patlàn A., Siler U., Bucciol G., Mekahli D., Vermeulen F., Desmet L., Maebe S., Flipts H., Corveleyn A., Moshous D., Philippet P., Tangye S.G., Boisson B., Casanova J.L., Florkin B., Struyf S., Reichenbach J., Bustamante J., Notarangelo L.D, & Meyts I. (2019). Human DOCK2 Deficiency: Report of a Novel Mutation and Evidence for Neutrophil Dysfunction. Journal of Clinical Immunology, 39(3), 298-308.
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