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2 protocols using monoclonal anti myc tag 2276 antibodies

1

Comprehensive Antibody Validation Protocol

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All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-ERG antibody [EPR3864 (2)] and (C-20) were purchased from Abcam and Santa Cruz, respectively. Anti-SPOP antibody (16750-1-AP), Anti-BRD2 (22236-1-AP) and anti-BRD3 (11859-1-AP) antibody were purchased from Proteintech. Anti-BRD4 antibody was purchased from Cell Signaling (13440) and Bethyl laboratories (A301-985A-M). Anti-Cullin 3 (2759), anti-GST (2625), anti-DEK (13962s), anti-SRC3 (2126), anti-Cleaved Caspase-3 (9664), anti-Cleaved PARP (5625), polyclonal anti-Myc-Tag antibody (2278) and monoclonal anti-Myc-Tag (2276) antibodies were purchased from Cell Signaling. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma. Monoclonal anti-HA antibody (MMS-101P) was purchased from Biolegend. Anti-GFP (8371-2) antibody was purchased from Clontech. Anti-Cullin 1 (SC-17775), anti-AR (N-20), anti-TRIM24/TIF1α (C-4), polyclonal anti-HA (SC-805) and anti-p27 (SC-528) were purchased from Santa Cruz. Anti-Xpress (46-0528) antibody was purchased from Invitrogen.
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2

Comprehensive Antibody Validation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-ERG antibody [EPR3864 (2)] and (C-20) were purchased from Abcam and Santa Cruz, respectively. Anti-SPOP antibody (16750-1-AP), Anti-BRD2 (22236-1-AP) and anti-BRD3 (11859-1-AP) antibody were purchased from Proteintech. Anti-BRD4 antibody was purchased from Cell Signaling (13440) and Bethyl laboratories (A301-985A-M). Anti-Cullin 3 (2759), anti-GST (2625), anti-DEK (13962s), anti-SRC3 (2126), anti-Cleaved Caspase-3 (9664), anti-Cleaved PARP (5625), polyclonal anti-Myc-Tag antibody (2278) and monoclonal anti-Myc-Tag (2276) antibodies were purchased from Cell Signaling. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma. Monoclonal anti-HA antibody (MMS-101P) was purchased from Biolegend. Anti-GFP (8371-2) antibody was purchased from Clontech. Anti-Cullin 1 (SC-17775), anti-AR (N-20), anti-TRIM24/TIF1α (C-4), polyclonal anti-HA (SC-805) and anti-p27 (SC-528) were purchased from Santa Cruz. Anti-Xpress (46-0528) antibody was purchased from Invitrogen.
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