Human epidermal keratinocytes and human dermal fibroblasts were obtained from Lonza (Walkersville, MD). Human epidermal keratinocytes were cultured and expanded in serum-free keratinocyte growth medium (Lonza), and human dermal fibroblasts were cultured and expanded in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, NY) with 10% (v/v) fetal bovine serum (FBS, Gibco BRL) and 1% (v/v) penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO). Cells were cultured at 37°C in a humidified 5% CO2 incubator. The medium was changed every 2–3 days until the use.
Human epidermal keratinocytes
Manufactured by Lonza
Sourced in United States
Human epidermal keratinocytes are primary cells derived from human skin tissue. They serve as a model system for the study of keratinocyte biology and skin physiology.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Human dermal fibroblasts, by Thermo Fisher Scientific (1 mentions)
Serum free keratinocyte growth medium, by Lonza (1 mentions)
Human dermal fibroblasts, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Kgm gold keratinocyte growth medium singlequots supplements and growth factors, by Lonza (1 mentions)
2 protocols using human epidermal keratinocytes
1
Culturing Human Skin Cells
2
Human Skin Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human adult hDFs and human epidermal keratinocytes were purchased from Lonza Walkersville Inc (Walkersville, MD, USA). hDFs were cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 1% penicillin-streptomycin and 10% fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, USA). For assays, hDFs at 3 passages were seeded on 12- or 48-well plates (BD, Franklin Lakes, NJ). human epidermal keratinocytes were cultured in KGM-Gold™ Keratinocyte Growth Medium SingleQuots™ Supplements and Growth Factors (Lonza, Basel, Switzerland). At 2 passages, these keratinocytes were seeded on 12- or 48-well plates (BD) for use in assays. Interleukin (IL)-1β (1 ng/ml) (AF-200-01B, PeproTech, Rocky Hill, NJ) was added 24 h after passage.
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