Thymii from 4 week old mice and thymic lymphomas from 10 week old mice were collected 48 hours after adenovirus infection. Single cells were obtained by homogenizing the thymii through a 0.75 μM filter. Cells were stained with CD3-PE (145-2C11), CD4-PerCP-Cy5.5 (RM4-5), CD8-APC (53-6.7), CD45-FITC (30-F11)(BDPharmingen), AnnexinV-Pacific Blue (A35122, Life Technologies), and 7-AAD (V35124, Invitrogen) and sorted using a BD Aria Cell Sorter or analyzed using the LSR Fortessa Cell Analyzer and FlowJo software.
Cd4 percp cy5.5 rm4 5
Manufactured by BD
Sourced in United States
CD4-PerCP-Cy5.5 (RM4-5) is a fluorescent-labeled antibody used in flow cytometry applications for the detection and enumeration of CD4-positive cells. It binds to the CD4 surface antigen expressed on a subset of T lymphocytes.
Automatically generated - may contain errors
Lab products found in correlation
7 aad, by Thermo Fisher Scientific (2 mentions)
Cd3 pe 145 2c11, by BD (2 mentions)
Annexinv pacific blue, by Thermo Fisher Scientific (2 mentions)
Cd8 apc 53 6.7, by BD (2 mentions)
Cd45 fitc 30 f11, by BD (2 mentions)
Anti cd11b percp cy5, by BD (1 mentions)
Anti cd11c apc hl3, by BD (1 mentions)
Anti cd86 pe, by BD (1 mentions)
Anti cd80 pe, by BD (1 mentions)
Anti f4 80 bv421, by BD (1 mentions)
Recombinant mouse gm csf, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Anti cd3 fitc, by BD (1 mentions)
3 protocols using cd4 percp cy5.5 rm4 5
1
Characterization of Thymus Cells
2
Immune Cell Activation and Characterization
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Lipopolysaccharide (LPS) (Escherichia coli, E. coli 055:B5) and PMA [phorbol 12-myristate 13-acetate ≥ 99% (TLC), film or powder] were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ionomycin (Free Base) was purchased from MKbio (Shanghai, China). Recombinant mouse GM-CSF (granulocyte-macrophage colony stimulating factor), IL-4 (interleukin 4) and IL-2 (interleukin 2) were obtained from Peprotech (Rocky Hill, NJ, USA). Cell staining was performed by using the following mAbs from BD Biosciences (Franklin Lakes, NJ, USA): anti-CD45 FITC (30-F11, #553079), anti-CD11b PerCP-Cy5.5 (M1/70, #550993), anti-CD11c APC (HL3, #550261), anti-F4/80 BV421 (T45-2342, #565411), anti-Ly-6G (Gr-1) PE (1A8, #551461), anti-CD80 PE (16-10A1, #553769), anti-MHC II BV421 (M5/114.15.2, #562564), anti-CD86 PE (GL1, #561963), anti-CD40 BV421 (3/23, #562846), anti-CD3 FITC (17A2, #561798), CD4 PerCP-Cy5.5 (RM4-5, #550954), anti-CD8a PerCP-Cy5.5 (53-6.7, #551162), anti-IL-2 PE (JES6-5H4, #554428), anti-TNFα BV421(MP6-XT22, #563387) and anti-IFNγ APC (XMG1.2, 554413). The isotype-matched mAbs for control staining were from BD Biosciences. Cytotoxicity LDH Assay Kit-WST was purchased from Dokindo (Tokyo, Japan).
3
Characterization of Thymus Cells
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