Anesthesia was induced with 3.0% Isoflurane in a gas mixture of N2O (70%) and O2 (30%) and maintained with 1.0% - 1.5% Isoflurane throughout the procedure. For intra-striatal fluorescent tracer (FITC-Dextran, 70 kD) and MRI contrast agent (Gd-albumin, 70 kD) infusion, anesthetized rats were placed in a stereotaxic frame (Stoelting Co.) and the skin was opened to expose the skull. A small burr hole was made over the right parietal cortex with a hand-held drill (Foredom Electric Co., Bethel, CT, USA). A 27-gauge needle was introduced into the right striatum at the following coordinates based on the rat brain atlas (Paxinos and Watson, 1997): anterior-posterior (AP) = 0 mm; midline (ML) = 3.5 mm; vertical depth (VD) = 5 mm from bregma. FITC-Dextran (3μl constituted in artificial CSF at a concentration of 1%) and MRI contrast agent, Gd-albumin (3μl) were loaded together into a PE-10 catheter (Becton Dickinson, MD, US) and part of the catheter was introduced through the needle track into the striatum. The skull burr hole was then sealed with bone wax. Rats were taken to the 7 Tesla (T) magnet for MR imaging immediately after the surgical procedure. The remaining part of the indwelling catheter was connected to another PE-10 catheter filled with saline and attached to a glass Hamilton syringe as an extension out of the MRI machine.
Pe 10 catheter
Manufactured by BD
Sourced in United States
The PE-10 catheter is a laboratory equipment designed for fluid transfer and sample collection. It is a small-bore polyethylene tube used for various applications in scientific research and experiments.
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Lab products found in correlation
9 protocols using pe 10 catheter
1
Dual Tracer Infusion in Rat Striatum
2
Intrathecal Catheterization in Rats
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All experiments were performed following the International Association for the Study of Pain guidelines for the Use of Animals in Research, and the experimental protocol was approved by the Institutional Animal Care and Use Committee of Chonnam National University (CNU IACUC-H-2017-10).
Male Sprague–Dawley rats (weight 220–250 g) were acclimated to the laboratory environment for 3 days before use. All animals had free access to a standard rat diet and tap water. The room temperature was maintained at 20°C–23°C with an alternating 12-hour light/dark cycle.
Under general anesthesia with sevoflurane, a polyethylene tubing with inner and outer diameters of 0.28 and 0.64 mm (PE-10 catheter; Becton Dickinson Co., Sparks, MD) was inserted into the intrathecal (i.t.) space through the atlanto-occipital membrane [10 (link)]. The PE-10 catheter was stretched out to reduce the size before use and advanced in a caudal direction until it reached the lumbar enlargement. The other end of the PE-10 catheter was exteriorized to the top of the head and plugged with a stainless-steel wire to prevent clogging of the catheter and later administration of experiment agents. Following i.t. catheterization, the animals were allowed to recover in individual cages for 5 days before further experiments.
Male Sprague–Dawley rats (weight 220–250 g) were acclimated to the laboratory environment for 3 days before use. All animals had free access to a standard rat diet and tap water. The room temperature was maintained at 20°C–23°C with an alternating 12-hour light/dark cycle.
Under general anesthesia with sevoflurane, a polyethylene tubing with inner and outer diameters of 0.28 and 0.64 mm (PE-10 catheter; Becton Dickinson Co., Sparks, MD) was inserted into the intrathecal (i.t.) space through the atlanto-occipital membrane [10 (link)]. The PE-10 catheter was stretched out to reduce the size before use and advanced in a caudal direction until it reached the lumbar enlargement. The other end of the PE-10 catheter was exteriorized to the top of the head and plugged with a stainless-steel wire to prevent clogging of the catheter and later administration of experiment agents. Following i.t. catheterization, the animals were allowed to recover in individual cages for 5 days before further experiments.
3
Spinal Cord Insertion Technique for Thermal Antinociception
4
Permanent Spinal Cord Cannulation for Drug Delivery
5
Evaluating CCR2 Antagonist for Diabetic Neuropathy
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At the time of gastric surgery for EMG recording, a PE10 catheter (Becton, Dickinson and Company, Franklin Lakes, NJ) was implanted into the intrathecal space via a small cut opening on the atlanto-occipital membrane. The tip of the catheter was placed at the T10 level and fixed in the correct position using a few sutures. After abdominal and/or intrathecal surgery, all the rats received ciprofloxacin (i.p., 20 mg/kg in 0.1 N HCl; Sigma-Aldrich, Tokyo, Japan) to prevent the type of infections that usually develop with DM.
To evaluate the effect of the drug, DM rats with ultrasensitive response to GD (10-fold more sensitive than the CT) were selectively placed in the therapeutic (pharmacology) group. Following EMG recording at two weeks after STZ or CB administration, the animals received a single intrathecal injection of CCR2 antagonist (25 µl of INCB3344 in 10% DMSO + saline; MedChem Express, Princeton, NJ) or vehicle (10% DMSO + saline). The VMR to GD was then recorded again at 1, 2, 4, and 24 h after injection.
To evaluate the effect of the drug, DM rats with ultrasensitive response to GD (10-fold more sensitive than the CT) were selectively placed in the therapeutic (pharmacology) group. Following EMG recording at two weeks after STZ or CB administration, the animals received a single intrathecal injection of CCR2 antagonist (25 µl of INCB3344 in 10% DMSO + saline; MedChem Express, Princeton, NJ) or vehicle (10% DMSO + saline). The VMR to GD was then recorded again at 1, 2, 4, and 24 h after injection.
6
Intrastriatal Tracer and Contrast Agent Infusion in Rats
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Anesthesia was induced with 3.0% Isoflurane in a gas mixture of N2O (70%) and O2 (30%) and maintained with 1.0–1.5% Isoflurane throughout the procedure. For intra-striatal fluorescent tracer (FITC-Dextran, 70 kD) and MRI contrast agent (Gd-albumin, 70 kD) infusion, anesthetized rats were placed in a stereotaxic frame (Stoelting Co., Wood Dale, IL, USA) and the skin was opened to expose the skull. A small burr hole was made over the right parietal cortex with a hand-held drill (Foredom Electric Co., Bethel, CT, USA). A 27-gauge needle was introduced into the right striatum at the following coordinates based on the rat brain atlas (Paxinos and Watson, 1997): anterior-posterior (AP) = 0 mm; midline (ML) = 3.5 mm; vertical depth (VD) = 5 mm from bregma. FITC-Dextran (3 μL constituted in artificial CSF at a concentration of 1%) and MRI contrast agent, Gd-albumin (3 μL) were loaded together into a PE-10 catheter (Becton Dickinson, MD, USA) and part of the catheter was introduced through the needle track into the striatum. The skull burr hole was then sealed with bone wax. Rats were taken to the 7 Tesla (T) magnet for MR imaging immediately after the surgical procedure. The remaining part of the indwelling catheter was connected to another PE-10 catheter filled with saline and attached to a glass Hamilton syringe as an extension out of the MRI machine.
7
Intrathecal Catheter Implantation in Rats
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Intrathecal cannulation was performed as previously described.15 (link) The rats were anesthetized using 5% isoflurane, and a 6-cm long PE-10 catheter (Becton Dickinson, Sparks, MD, USA) was implanted into the subarachnoid space at the L4–L5 level. Isoflurane was administered when necessary during the operation. Tail or hind-limb movements when unconscious were considered as signs of dura penetration. The catheter was then pushed further by 1.5 cm into the subarachnoid space, and the end of the catheter was heat sealed. On the following day, 10 μL of 2% lidocaine was injected to confirm the accuracy of the catheter position. The rats are allowed to recover for 3 days.
8
Chronic Subarachnoid Catheter Implantation
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Rats were implanted with chronic indwelling catheters in the subarachnoid space on the same day after CCI procedure. Briefly, rats were anesthetized with sodium pentobarbital (40 mg/kg, i.p.). A PE-10 catheter (Becton Dickinson, Sparks, MD, USA) was inserted into the lumbar subarachnoid space between lumbar vertebrae 5 (L5) and L6 [19 (link)]. The catheter was chronically implanted and the external part of the indwelling catheter was protected according to Milligan's method [20 (link)]. A lidocaine test was given to determine the functionality and position of the catheter tip in the subarachnoid space.
9
Intrathecal Catheter Implantation in Rats
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Rats were anesthetized with sodium pentobarbital (40 mg/kg, i.p.). A PE-10 catheter (Becton Dickinson, Sparks, MD, USA) was inserted into the lumbar subarachnoid space between lumbar vertebrae 5 (L5) and L6 [13 (link)]. The catheter was chronically implanted and the external part of the indwelling catheter was protected according to Milligan's method [14 (link)]. A lidocaine test was given to determine the functionality and position of the catheter tip in the subarachnoid space.
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