All animal groups were subjected to determinate gastric ulcer indices; lesion counts, total acidity, and gastric volume. Oxidative stress markers; malondialdehyde (MDA) (Buege and Aust, 1978 (link)), superoxide dismutase (SOD) (Nishikimi et al., 1972 (link)) and glutathione (GSH) (Moron et al., 1979 (link)) were estimated in gastric tissue. Certain marker enzymes; succinate dehydrogenase (SDH) (mitochondrial marker) (Rice and Shelton (1957) (link), lactate dehydrogenase (LDH) (cytoplasm marker) (Babson and Babson, 1973 (link)), gluose-6-phosphatase (G-6-Pase) (microsomal marker) (Swanson, 1955 ), acid phosphatase (AP) (lysosomal marker) (De Duve and Wattiaux, 1956 (link)) and 5′- nucleotidase (5′NT) (plasma membrane marker) (Bodansky and Schwartz, 1963 (link)) were also carried out in gastric tissue. Inflammatory index were estimated by ELISA technique; interleukin-10 (IL-10) (Abcam, ab100765, Cambridge, USA), intracellular adhesion molecule-1 (ICAM-1) (Abcam, ab100763, Cambridge, USA), and tumor necrosis factor alpha (TNF-α) (Abcam, ab100785, Cambridge, USA) were also evaluated in gastric tissue. Gastric total protein was also estimated (Bradford, 1976 (link)).
Ab100763
Manufactured by Abcam
Sourced in United States
Ab100763 is a laboratory equipment product offered by Abcam. It is a device used for scientific research and analysis purposes. The core function of this product is to facilitate specific experimental or measurement tasks within a laboratory setting.
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2 protocols using ab100763
1
Gastric Ulcer Evaluation and Oxidative Stress
2
Bisphenol-A Lung Oxidative Stress Evaluation
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Bisphenol-A (BPA, Cat # 80–05–7), NaHS (Cat # 161,527) and all other substances were bought from Sigma-Aldrich Chemical Co. in St. Louis, Missouri, USA. Lipid peroxidation is represented by MDA (malondialdehyde) is measured according to the method of Yoshioka et al. [14 (link)] through a forming thiobarbituric acid reactive substance (TBARS) reading at 532 nm. The SOD (Superoxide dismutase) and GSH-PX (glutathione peroxidase) levels were determined by Kakkar et al. [15 (link)] and Mohandas et al. [16 (link)] respectively. Rat ELISA kits were used, according to the manufacturer’s instructions, the lung VEGF (Vascular endothelial growth factor, CAT no.RRV00) R&D Systems Minneapolis, MN, lung MCAF ( monocyte chemotactic and activating factor, CSB-E07429r), The activity of ICAM (intercellular adhesion molecule -1, ab100763) Abcam, Cambridge, MA, USA, lung VCAM-1(vascular cell adhesion protein 1, CSB-E07275r), the level of, IFN-β (Interferon-β, CSB-E04845r), VIM (vimentin, CSB-E14029r). Rat TGF-β1 (Transforming Growth Factor β1, CSB-E04727r), MMP-2 and MMP-9 (matrix metalloproteinase 2 and 9, CSB-E07411r, CSB-E08008r) and TIMP-1 (Tissue inhibitors of metalloproteinase 1, CSB-E08005r) levels were measured using ELISA kits supplied by CUSABIO® Technology, LLC. The samples were determined using an ELISA plate reader from BioTek, Vermont, U.S.A.
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