Percentage DNA methylation was assessed at CpG sites across CpG islands in CYP2R1, CYP27A1, CYP27B1, CYP24A1, and VDR genes using Qiagen EpiTect II methylation enzyme kits and PCR assays at the concentrations and cycle conditions recommended by the manufacturer. Details of the CpG island size, location, and CpG content are described in the supplementary methods (Supplementary Table S3 ). This system uses selective digestion of sample DNA with methylation-sensitive and methylation-dependent restriction enzymes, followed by quantification of the remaining DNA using real-time PCR. Details of the reaction conditions, CpG sites assayed, and target site primers (Supplementary Table S3 ) are included in the supplementary methods.
Epitect 2 methylation enzyme kits
Manufactured by Qiagen
The EpiTect II methylation enzyme kits are a set of laboratory tools designed for the analysis of DNA methylation. These kits provide the necessary enzymes and reagents to perform specific methylation-related experiments in a research setting. The core function of these kits is to facilitate the study of DNA methylation patterns, a process that plays a crucial role in gene expression and cellular regulation.
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Lab products found in correlation
2 protocols using epitect 2 methylation enzyme kits
1
Methylation Analysis of Vitamin D Pathway Genes
2
Quantification of VDR Gene Methylation
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Percentage DNA methylation (methylation density) was assessed at the CpG island of the VDR gene using Qiagen EpiTect II methylation enzyme kits and PCR assays at the concentrations and cycle conditions recommended by the manufacturer [Holemon et al., 2007] , and as reported by Beckett and colleagues [Beckett et al., 2016a] . The VDR CpG island (ID no: 103069) spans the promotor region, transcription start site and part of the gene body (Chr 12:48298645-48299537), covering 892 base pairs including 72 CpG sites.
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