Anthropometric measurements were collected in the present study, including height, weight, waist and hip circumference. The body mass index (BMI) was calculated as the weight divided by the square of the height (kg/m2). Waist–hip ratio (WHR) was calculated as waist circumference divided by hip circumference. Biochemical parameters were detected in previously stored plasma samples. The plasma levels of total cholesterol (TC), triacylglycerol, high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDLC) concentrations, fasting blood glucose (FBG), creatinine, blood urea nitrogen (BUN) and PSA were measured using the standard methods by a chemistry analyzer (Shimadzu, cl8000, Japan).
Cl 8000
Manufactured by Shimadzu
Sourced in Japan
The CL-8000 is a chemiluminescence detector designed for high-sensitivity analysis of luminescent compounds. It features a photomultiplier tube detector and a temperature-controlled flow cell to ensure stable and reproducible measurements. The device is suitable for applications that require the detection of low-level luminescent species.
Automatically generated - may contain errors
5 protocols using cl 8000
1
Anthropometric and Biochemical Measurements
2
Assessing Kidney Function in Rats
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Blood samples were collected for BUN and serum creatinine (SCr) measurements on days 0, 3 and 5 following CP injection. On days 0 and 3, 1 ml blood was collected from the orbital venous plexus after inhalation anesthesia with 5% isoflurane. Blood collection was performed within 2 min. Rats were then put back to their cages to keep raising after compression hemostasis and provided free access to water and normal rat chow. On day 5, 2 ml blood was collected from the auricula dextra after anesthesia with an intraperitoneal injection of 2% pentobarbital sodium (45 mg/kg) for anesthesia, where the blood collection was done within 10 min. Rats were sacrificed by exsanguination after blood collection. Death was confirmed by the absence of heartbeat. BUN and SCr levels were determined using an automatic biochemical analyzer (Shimadzu Corporation; CL-8000) at the Chinese Academy of Sciences Guangzhou Institute of Biomedicine and Health Laboratory Animal Center.
3
Automated Hepatocyte Function Analysis
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ALB and urea secretion of differentiated hepatocytes were analysed using fully automatic chemistry analyzer (SHIMADZU CL-8000).
4
Comprehensive Blood Analysis Protocol
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The hemoglobin concentration, hematocrit level, red blood cell count, mean cell volume, mean corpuscular hemoglobin, and mean cell hemoglobin concentration in blood samples drawn from the heart were measured using an automatic hematology analyzer (KX-21NV; Sysmex, Kobe, Japan). The partial pressures of oxygen (pO2) and carbon dioxide (pCO2) were measured using an analyzer (i-STAT; Abbott Point of Care, IL, USA). Lactic acid in the blood samples drawn from the tail was measured using a simplified analyzer (Lactate Pro; Arkray, Kyoto, Japan).
Blood samples were centrifuged at 1,500 g for 10 min at 4°C to obtain serum samples. Serum haptoglobin was measured using an ELISA Kit (Life Diagnostics, PA, USA). The serum hemoglobin level was measured using an assay kit (Hemoglobin Colorimetric Assay Kit; Cayman Chemical, MI, USA). The serum level of creatine kinase was quantified using a kit (Max Discovery; Bioo Scientific, USA). Serum iron, the unsaturated iron-binding capacity (UIBC), and lactate dehydrogenase were measured using Detaminer Fe and UIBC (Kyowa Medix, Tokyo, Japan) and LDH–L (Serotec, Sapporo, Japan) with an automatic biochemical analyzer (CL-8000; Shimadzu, Kyoto, Japan). The total iron-binding capacity (TIBC) and serum transferrin saturation were calculated as follows:
Serum transferrin saturation = serum iron/TIBC × 100
Blood samples were centrifuged at 1,500 g for 10 min at 4°C to obtain serum samples. Serum haptoglobin was measured using an ELISA Kit (Life Diagnostics, PA, USA). The serum hemoglobin level was measured using an assay kit (Hemoglobin Colorimetric Assay Kit; Cayman Chemical, MI, USA). The serum level of creatine kinase was quantified using a kit (Max Discovery; Bioo Scientific, USA). Serum iron, the unsaturated iron-binding capacity (UIBC), and lactate dehydrogenase were measured using Detaminer Fe and UIBC (Kyowa Medix, Tokyo, Japan) and LDH–L (Serotec, Sapporo, Japan) with an automatic biochemical analyzer (CL-8000; Shimadzu, Kyoto, Japan). The total iron-binding capacity (TIBC) and serum transferrin saturation were calculated as follows:
Serum transferrin saturation = serum iron/TIBC × 100
5
Serum Antioxidant and Inflammatory Markers
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According to kits’ instructions, serum enzyme activities of SOD, GSH-Px, and CAT, serum T-AOC were analyzed using a CL-8000 automatic autoanalyzer Shimadzu Company (Shanghai, China) and serum IL-1β, IL-6, IL-4, IL-8, IL-10, IL-17, IFN-γ, PGE2, Ig A, Ig M, Ig G, and IGF-1 concentrations were measured using porcine-specific ELISA kits.
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