Gfap primary antibody

Manufactured by Merck Group

GFAP (Glial Fibrillary Acidic Protein) primary antibody is a laboratory reagent used to detect and quantify GFAP in various biological samples. GFAP is a type III intermediate filament protein that is expressed in astrocytes and other glial cells. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and analyze GFAP-positive cells.

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Lab products found in correlation

Phosphate buffered saline (pbs), by Santa Cruz Biotechnology (1 mentions) Sp8 confocal microscope, by Leica (1 mentions) Fibrinogen, by Agilent Technologies (1 mentions) Bovine serum albumin (bsa), by Santa Cruz Biotechnology (1 mentions) Eclipse te2000 u, by Leica (1 mentions) Prolong gold with dapi, by Thermo Fisher Scientific (1 mentions) Alexa 488 green, by Thermo Fisher Scientific (1 mentions) Alexa 568 red, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Primary monoclonal anti-GFAP mouse antibody CY3-conjugated primary antibody against GFAP GFAP antibody

2 protocols using gfap primary antibody

GFAP Immunostaining of 2D and 3D Samples

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The samples were fixed at different time points with 4% paraformaldehyde (PFA) solution in PBS (Santa Cruz Biotechnology). Fixation time was 10 min for 2D samples and 20–30 min for the 3D constructs. The cells were stained with glial fibrillary acidic protein (GFAP)primary antibody (Sigma G3893) at a 1:500 dilution and incubations were performed at 4 °C overnight, while the secondary antibody (Invitrogen A-11001, 1:250 dilution) incubations were carried out at room temperature for 2 h. The stained samples were imaged using the Leica SP8 confocal microscope with ×10 or ×25 objectives.

Sood D., Tang-Schomer M., Pouli D., Mizzoni C., Raia N., Tai A., Arkun K., Wu J., Black LD I.I.I., Scheffler B., Georgakoudi I., Steindler D.A, & Kaplan D.L. (2019). 3D extracellular matrix microenvironment in bioengineered tissue models of primary pediatric and adult brain tumors. Nature Communications, 10, 4529.

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Immunofluorescence Staining of Paraffin Sections

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6μm paraffin sections were incubated with GFAP primary antibody (1:400 dilution, GA-5, Sigma), or Fibrinogen (1:100 dilution, Dako) overnight at 4°C. Following washes with PBS with 0.2% bovine serum albumin (Santa Cruz) (PBS/BSA), they were incubated with secondary antibodies directly conjugated with Alexa 488 (green) or Alexa 568 (red) (Molecular Probes/Invitrogen, Carlsbad, CA), and cover-slipped with Prolong Gold with DAPI (Molecular Probes/Invitrogen). Stained slides were imaged fluorescence microscope (Nikon Eclipse TE2000-U) or confocal (Leica TCS2, Figure 2 only).

Lee K.M., Chiu K.B., Sansing H.A., Didier P.J., Lackner A.A, & MacLean A.G. (2016). The Flavivirus Dengue induces hypertrophy of white matter astrocytes. Journal of neurovirology, 22(6), 831-839.

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