Pcdna3.1 control vector

siSNHG7, siSYVN1 and their negative control (scramble) as well as pcDNA3.1-control (vector) and pcDNA3.1-SNHG7 (lncRNA SNHG7) were purchased from GenePharma (Shanghai, China). MiR-34a-5p inhibitor (anti-miR-34a-5p), miR-34a-5p mimics (miR-34a-5p) and their negative control (anti-NC and NC) were also purchased from GenePharma (Shanghai, China). These plasmids and oligos were transfected into OA cells using Lipofectamine 3000 Reagents (Invitrogen).

pcDNA3.1-control (vector) and pcDNA3.1-RUNX1-IT1 (RUNX1-IT1) were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China) as previously reported^{24 (link)}. Scrambled shRNA (shControl) and RUNX1-IT1 shRNAs (shRNA#1 and shRNA#2) were obtained from Invitrogen (Carlsbad, CA, USA). MiR-632 inhibitors and miR-632 mimics were synthesised from Genecopoeia (Guangzhou, China). The siRNAs against GSK-3β, HIF-1α and HDAC3 were designed and synthesised by Shanghai GenePharma Co., Ltd. (Shanghai, China). The sequences were as follows: si-GSK-3β/sense 5′-CGAUUACACGUCUAGUAUA-3′ and si-GSK-3β/antisense 5′- UAACAAUCUAUUUACACCC-3′; si-HDAC3/sense 5′-UCGCCUGGCAUUGACCCAUTT-3′ and si-HDAC3/antisense 5′-AUGGGUCAAUGCCAGGCGATT-3′; si-HIF-1α/sense 5′-UGUGAGUUCGCAUCUUGAUTT-3′ and si-HIF-1α/antisense 5′-TTACACUCAAGCGUAGAACUA-3′. The GSK-3β Human cDNA ORF Clone (GSK-3β) was purchased from OriGene (OriGene Technologies, Inc., USA). Cell transfection was conducted as previously described^{25 (link)}. Forty-eight hours post transfection, the cells were utilised for further experiments.