Iota-, kappa- and lambda-carrageenan were purchased from Dupont former FMC Biopolymers (both Philadelphia, PA, USA). Fucoidan from Undaria pinnatifolia and Fucus vesiculosis were from Marinova (Marinova Pty Ltd., Cambridge TAS, Australia), Carboxymethylcelluslose (CMC) was from Mare Austria GmbH, Niklasdorf, Austria Hydroxypropylmethylcellulose (HPMC) from Fagron (Fagron BV, Rotterdam, The Netherlands). The dry polymer powders were dissolved in cell culture water (B Braun Melsungen AG, Melsungen, Germany) to a final concentration of 2.4 mg/mL containing 0.5% NaCl (Merck KGA, Darmstadt, Germany). This stock solution was sterile filtered through a 0.22 mm filter (Sarstedt, Nümbrecht, Germany) and stored at 4 °C until use.
0.22 mm filter
Manufactured by Sarstedt
Sourced in Germany
The 0.22 mm filter is a laboratory equipment used for filtration purposes. It has a pore size of 0.22 millimeters, which allows for the removal of particulates and microorganisms from liquids.
Automatically generated - may contain errors
Lab products found in correlation
Cell culture water, by B. Braun (4 mentions)
Sodium chloride (nacl), by Merck Group (3 mentions)
Hydroxypropylmethylcellulose hpmc, by Fagron (2 mentions)
Galactose 4 sulfate, by Merck Group (2 mentions)
Remdesivir, by Cayman Chemical (1 mentions)
Hydroxychloroquine, by Cayman Chemical (1 mentions)
Lectin from triticum vulgaris, by Merck Group (1 mentions)
5 protocols using 0.22 mm filter
1
Preparation of Polysaccharide Solutions
2
Extracellular Matrix Gel Extraction
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ECM/SVF-gel extract was prepared according to the manufacturer's instructions [13 (link)]. Briefly, in vivo ECM/SVF-gel samples from all 5 time points were harvested. The samples were cut into small pieces and mixed with an equal volume of Dulbecco's modified Eagle's medium. The mixture was incubated for 24 h at 37°C in a CO2 incubator. ECM/SVF-gel extracts were collected after the mixture was centrifuged at 12,000 rpm for 5 min and sterile-filtered through a 0.22 mm filter (Sarstedt, Nümbrecht, Germany). The extracts were stored at −20°C until use.
3
Antiviral Biopolymer Solutions for SARS-CoV-2
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Iota-, kappa- and lambda-carrageenan were purchased from Dupont former FMC Biopolymers (both Philadelphia, PA). Fucoidan from Undaria pinnatifolia and Fucus vesiculosis were from Marinova (Marinova Pty Ltd, Australia), Carboxymethylcelluslose (CMC) was from Mare Austria GmbH, Hydroxypropylmethylcellulose (HPMC) from Fagron (Fagron BV, The Netherlands), galactose-4-sulfate was purchased from Merck KGA (Germany). The dry polymer powders were dissolved in cell culture water (B Braun Melsungen AG, Germany) to a final concentration of 2.4 mg/ml containing 0.5% NaCl (Merck KGA, Germany). This stock solution was sterile filtered through a 0.22mm filter (Sarstedt, Germany) and stored at 4°C until use.
For testing of wild type of wild type SARS-CoV-2 virus a commercially available nasal spray containing 1.2 mg/ml iota-carrageenan was used.
For testing of wild type of wild type SARS-CoV-2 virus a commercially available nasal spray containing 1.2 mg/ml iota-carrageenan was used.
4
Preparation of Viral Inhibitor Compounds
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Lectin from Triticum vulgaris was obtained from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in PBS, resulting in a stock solution of 1 mg/mL. Hydroxychloroquine was acquired as a pure substance (Cayman, Ann Arbor, MI, USA) and dissolved in PBS with a stock solution of 11.5 mM. Remdesivir was obtained from Cayman Chemical (Ann Arbor, MI, USA) and dissolved in DMSO, resulting in a stock solution of 1 mM. All interventions were used at the concentrations indicated in the different experiments.
Iota-carrageenan was used as positive control in some assays and was purchased from Dupont former FMC Biopolymers (both Philadelphia, PA, USA). The dry polymer powders were dissolved in cell culture water (B Braun, Melsungen, Germany) to a final iota-carrageenan concentration of 2.4 mg/mL containing 0.5% NaCl (Merck KGA, Darmstadt, Germany). This stock solution was sterile filtered through a 0.22 mm filter (Sarstedt, Nümbrecht, Germany) and stored at 4 °C until use.
Iota-carrageenan was used as positive control in some assays and was purchased from Dupont former FMC Biopolymers (both Philadelphia, PA, USA). The dry polymer powders were dissolved in cell culture water (B Braun, Melsungen, Germany) to a final iota-carrageenan concentration of 2.4 mg/mL containing 0.5% NaCl (Merck KGA, Darmstadt, Germany). This stock solution was sterile filtered through a 0.22 mm filter (Sarstedt, Nümbrecht, Germany) and stored at 4 °C until use.
5
Preparation of Polysaccharide Stock Solutions
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Iota-, kappa-and lambda-carrageenan were purchased from Dupont former FMC Biopolymers (both Philadelphia, PA). Fucoidan from Undaria pinnatifolia and Fucus vesiculosis were from Marinova (Marinova Pty Ltd, Australia), CMC was from Mare Austria GmbH, HPMC from Fagron (Fagron BV, The Netherlands), galactose-4-sulfate was purchased from Merck KGA (Germany).
The dry polymer powders were dissolved in cell culture water (B Braun Melsungen AG, Germany) to a final concentration of 2.4 mg/ml containing 0.5% NaCl (Merck KGA, Germany). This stock solution was sterile filtered through a 0.22mm filter (Sarstedt, Germany) and stored at 4°C until use.
The dry polymer powders were dissolved in cell culture water (B Braun Melsungen AG, Germany) to a final concentration of 2.4 mg/ml containing 0.5% NaCl (Merck KGA, Germany). This stock solution was sterile filtered through a 0.22mm filter (Sarstedt, Germany) and stored at 4°C until use.
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