Total protein was harvested by radioimmunoprecipitation assay (high) (Beyotime, Shanghai, China). A BCA protein assay kit (Jining Shiye, Shanghai, China) was used to detect protein content. Protein was separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane (Tengxiang, Yueqing, Wenzhou, Zhejiang, China). The membranes were sealed using tris‐buffered saline plus tween 20 containing 5% non‐fat milk at room temperature for 90 minutes, and hybridized to anti‐GLI1 (ab134906) and anti‐GAPDH (ab157156) (dilution: 1:1200; Abcam, Cambridge, UK) at 4°C overnight. Corresponding secondary antibodies (rabbit anti‐mouse immunoglobulin G [IgG], Cell Signaling Technology, Danvers, MA, USA; #58802, 1:7000; goat anti‐mouse IgG, ab6785, 1:7000, Abcam) were added to bind to the membranes at 37°C for 60 minutes. The blots were exposed by a WFH‐101B gel imaging analysis system (Jinke, Shanghai, China).
Rabbit anti mouse immunoglobulin g igg
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-mouse immunoglobulin G (IgG) is a laboratory reagent used in immunological experiments. It is an antibody produced in rabbits that specifically binds to mouse IgG antibodies. This reagent can be used to detect and quantify mouse IgG in samples.
Automatically generated - may contain errors
Lab products found in correlation
Ab157156, by Abcam (1 mentions)
Ab134906, by Abcam (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Mouse polyclonal anti human bcl 2, by Cell Signaling Technology (1 mentions)
Mouse polyclonal anti gapdh, by Cell Signaling Technology (1 mentions)
Coumarin 6 cou 6, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by CoWin Biotech (1 mentions)
Goat anti rabbit igg, by Cell Signaling Technology (1 mentions)
Shikonin, by Yuanye Bio-Technology (1 mentions)
2 protocols using rabbit anti mouse immunoglobulin g igg
1
Protein Quantification and Western Blot
2
Shikonin-Loaded Targeted Nanoparticles for Cancer Treatment
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Shikonin (assay 99.8%) was obtained from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). DSPE-PEG2000-RGD (purity > 97%) was synthesized by Xi'an ruixi Biological Technology Co., Ltd. (Xi’an, China). DSPE-PEG2000 was purchased from ToYong Biotch Co., Ltd. (Shanghai, China). Egg phosphatidylcholine (EPC, assay 98%) was obtained from LIPOID GmbH (Ludwigshafen, Germany). Cholesterol (Chol), sephadex G-50, coumarin-6 (Cou6), and Hoechst 33258 were all purchased from Sigma-Aldrich (Shanghai, China). Annexin V–FITC/PI apoptosis detection kit was purchased from Beijing ComWin Biotech Co., Ltd. (Beijing, China). 3-(4,5-Dimethylth-iazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Gibco Brl (Shanghai, China). Rabbit polyclonal anti-human-MMP-2 and rabbit polyclonal anti-human-MMP-9 were obtained from Proteintech Group, Inc. (Chicago, IL, USA). Rabbit polyclonal anti-human-NF-κB p65, mouse polyclonal anti-human-Bcl-2, mouse polyclonal anti-human-Bax, mouse polyclonal anti-GAPDH, rabbit anti-mouse immunoglobulin G (IgG)and goat anti-rabbit IgG were all purchased from Cell Signaling Technology, Inc. (Boston, MA, USA).
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