Avatar 330 ft ir

To investigate the interactions between the drug and the excipients used in the preparation of the film, Fourier transform infrared spectroscopy (FTIR) was used. The infrared spectra of the drug, excipients, and polymer films were obtained using an FTIR (Avatar 330 FTIR, Thermo Fisher Scientific Inc., Waltham, MA, USA) spectrophotometer coupled with Zn/Se horizontal attenuated total reflectance (HATR) equipment. The films were laid on a clean crystal of the apparatus and scanned for absorbance in the wavelength range of 600 to 4000 cm⁻¹. The spectra were obtained from 128 scans with a spectral resolution of 32 cm⁻¹ and applying H₂O and CO₂ corrections. SpectraGryph (version 1.2.15.; Dr. Friedrich Menges Software, Entwicklung, Obersdorf, Germany) was used in the evaluation of the results.

NMR data (¹H, ¹³C{¹H}) were recorded on a 400 MHz Varian Unity Inova spectrometer. IR spectroscopy was performed using a Thermo Nicolet Avatar 330 FT-IR with ATR crystal. Mass spectrometry was performed on a Waters Synapt G2 with an ESI probe in ESI positive mode using a Cone Voltage of 15 V. HPLC data were recorded on an Agilent infinity II system. Flash chromatography was done using a Biotage Isolera One. Cyclic voltammograms were recorded using a NuVant System EZstatpro. UV-vis was performed using the Agilent Cary 3500 UV-Vis Spectrophotometer. Healthy human cell lines HEK293 and PNT1A were supplied by Dr CH Kaschula (University of Stellenbosch). Growth of HEK293 and PNT1A cells was monitored using a BioTek Synergy HTX multi-mode plate reader. T. gondii ΔKu80:mNeon cells used were provided by Dr CR Harding. Growth inhibition assays of T. gondii were monitored using a BMG LabTech PHERAstar FS microplate reader. Fluorescence activated cell sorting was performed using a Celesta FACS instrument.