Peroxidase based chemiluminescence detection kit

Manufactured by Cytiva

Sourced in United Kingdom

The Peroxidase-based chemiluminescence detection kit is a laboratory equipment product that enables the detection and visualization of peroxidase-labeled targets through a chemiluminescence-based assay. The kit provides the necessary reagents and protocols to perform this analysis.

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Lab products found in correlation

Ox camkii, by Merck Group (2 mentions) Chemidoc imaging system, by Bio-Rad (1 mentions) Phospholamban, by Abcam (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Bcl 2, by Santa Cruz Biotechnology (1 mentions) Anti il 1β, by Merck Group (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti enos, by Merck Group (1 mentions) Anti nnos, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Chemiluminescence kit (49 citations) Chemiluminescence detection kit (43 citations)

3 protocols using peroxidase based chemiluminescence detection kit

Oxidative Stress in Cardiomyocyte Signaling

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Rat ventricular cardiomyocytes were incubated during 60 minutes in control, ouabain 2 µmol/L or istaroxime 10 µmol/L. Then, cells were collected and homogenized with lysis buffer. Protein was measured by the Bradford method using BSA as standard. Lysates (≈90 μg of total protein per gel line) were seeded in a 10% SDS polyacrylamide gel and transferred to polyvinylidene difluoride membranes. Blots were probed overnight with antibodies raised against oxidized CaMKII (Ox‐CaMKII, 1:1000; Millipore Corp, Billerica, MA), phospholamban (1:1000; Badrillal Leeds, UK) and Anti Bax (Bax, 1:500; Abcam, Cambridge, MA). After stripping the blots were probed with phospho‐Thr286‐CaMKII (1:1000; Abcam) phospho‐Thr17‐ phospholamban (p‐Thr17 phospholamban, 2:500; Badrilla), Bcl‐2 (Bcl‐2, 1:1000; Santa Cruz Biotechnology, Dallas, TX) and anti‐GAPDH (GAPDH, 1:10000; Santa Cruz Biotechnology) was used as loading control for normalization.
Immunoreactivity was visualized by a peroxidase‐based chemiluminescence detection kit (Amersham Biosciences, Amersham, UK) using a Chemidoc Imaging System (Bio‐Rad Laboratories, Hercules, CA). The signal intensity of the bands in the immunoblots was quantified by densitometry using Image J software.¹⁸ (link)

Racioppi M.F., Burgos J.I., Morell M., Gonano L.A, & Vila Petroff M. (2021). Cellular Mechanisms Underlying the Low Cardiotoxicity of Istaroxime. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 10(14), e018833.

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Oxidized CaMKII Regulation by IL-1β

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Left ventricle tissue samples were incubated for 24 h in the presence or absence of 10 ng ml⁻¹ IL-1β and subsequently homogenized. Protein was measured by the Bradford method using BSA as standard. Lysates (∼90 μg of total protein per gel line) were seeded in a 10% SDS polyacrylamide gel and transferred to polyvinylidene difluoride membranes. Blots were probed overnight with antibodies raised against oxidized CaMKII (referred as Ox-CaMKII), Cat# 07-1387, 1:1,000 (Millipore Corp, USA). After stripping the blots were probed with phospho-Thr286-CaMKII (referred as p-CaMKII), Cat# 32678 1:1,000 (Badrilla, UK), Anti IL-1β Cat# I3767 1:1,000 (Sigma, USA) and Anti GAPDH, Cat# MAB374, 1:2,000 (Santa Cruz Biotechnology, USA) was used for normalization. Immunoreactivity was visualized by a peroxidase-based chemiluminescence detection kit (Amersham Biosciences) using a Chemidoc Imaging System. The signal intensity of the bands in the immunoblots was quantified by densitometry using the Image J software (NIH, USA).

Monnerat G., Alarcón M.L., Vasconcellos L.R., Hochman-Mendez C., Brasil G., Bassani R.A., Casis O., Malan D., Travassos L.H., Sepúlveda M., Burgos J.I., Vila-Petroff M., Dutra F.F., Bozza M.T., Paiva C.N., Carvalho A.B., Bonomo A., Fleischmann B.K., de Carvalho A.C, & Medei E. (2016). Macrophage-dependent IL-1β production induces cardiac arrhythmias in diabetic mice. Nature Communications, 7, 13344.

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eNOS and nNOS phosphorylation analysis

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Cardiac myocytes seeded onto 25 mm Petri dishes were maintained during 30 min in IS or HS in the absence or presence of 10 µM of Dorsomorphin. Then, cells were collected and homogenized with lysis buffer. Proteins were measured by the Bradford method using BSA as standard. Homogenates (~ 90 µg of total protein per gel line) were seeded in a 10% SDS polyacrylamide gel and transferred to polyvinylidene difluoride membranes. Blots were probed overnight with antibodies raised against anti-phospho-Ser1177 endothelial NO synthase (Cell Signaling, dilution 1:1000), anti-phospho-Ser1417 neuronal NO synthase (Abcam, dilution 1:2000), and anti-eNOS (Sigma-Aldrich, dilution 1:1000) and anti-nNOS (Santa Cruz, dilution 1:500) for normalization. Immunoreactivity was visualized by a peroxidase-based chemiluminescence detection kit (Amersham Biosciences) using a Chemidoc Imaging System. The signal intensity of the bands in the immunoblots was quantified by densitometry using the Image J software (NIH).

Morell M., Burgos J.I., Gonano L.A, & Vila Petroff M. (2018). AMPK-dependent nitric oxide release provides contractile support during hyperosmotic stress. Basic research in cardiology, 113(1).

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