Dextran from Leuconostoc spp. 500 000 (Sigma-Aldrich), Ficoll-Paque Plus d = 1.077 g/ml (GE Healthcare,), human eosinophil isolation kit (MiltenyiBiotec), DPBS (Dulbecco’s phosphate-buffered saline, Gibco), 10% fetal bovine serum (FBS, Gibco), penicillin–streptomycin (10 000 U/mL, Thermo-Fisher Scientific), Roswell Park Memorial Institute (RPMI) 1640 medium with L-glutamine and 25 mmol/l HEPES (Gibco), EDTA (Sigma-Aldrich), butyric acid (BA, Sigma-Aldrich); TNF-α (Tumour Necrosis Factor, Sigma-Aldrich), Il-1β (Interleukin 1β, Sigma-Aldrich) and LPSs (lipopolysaccharides, Sigma-Aldrich).
Butyric acid ba
Manufactured by Merck Group
Sourced in United States
Butyric acid (BA) is a short-chain fatty acid commonly used in laboratory applications. It is a colorless liquid with a distinctive odor. BA is a carboxylic acid with the chemical formula CH3CH2CH2COOH. This compound is a naturally occurring substance found in various foods and is also produced synthetically for industrial purposes.
Automatically generated - may contain errors
Lab products found in correlation
Dextran from leuconostoc spp 500 000, by Merck Group (1 mentions)
Human eosinophil isolation kit, by Miltenyi Biotec (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions)
Ficoll paque plus, by GE Healthcare (1 mentions)
Propionic acid, by Merck Group (1 mentions)
N 3 dimethylamino propyl n ethylcarbodiimide edc hcl, by Merck Group (1 mentions)
Mili q, by Merck Group (1 mentions)
Mili q water, by Merck Group (1 mentions)
Anhydrous pyridine, by Merck Group (1 mentions)
Caproic acid, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Isocaproic acid, by Merck Group (1 mentions)
2 ethyl hexanoic acid 2 eha, by Merck Group (1 mentions)
2 4 diisopropylphenol, by Merck Group (1 mentions)
2 6 diisopropylphenol, by Merck Group (1 mentions)
N n dicyclohexylcarbodiimide dcc 4 dimethylaminopyridine dmap, by Merck Group (1 mentions)
Precoated tlc plates, by Merck Group (1 mentions)
3 protocols using butyric acid ba
1
Isolation and Activation of Human Eosinophils
2
Quantitative Analysis of SCFAs
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All short-chain fatty acids standards ((acetic acid-AA (C2), propionic acid- PA (C3), butyric acid-BA (C4), isobutyric acid-IBA (C4), valeric acid- VA (C5), isovaleric acid-IVA (C5), caproic acid—CA (C6), isocaproic acid ICA (C6)) and short-chain fatty acid isotope-labeled standards (acetic acid-13C2, propionic acid-D6, butyric acid-13C2, isobutyric acid-D7, valeric acid-D9), pyridine anhydrous, 2-nitro phenylhydrazine (3NPH·HCl), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC·HCl)) were acquired from Sigma-Aldrich (St. Louis, MO, USA). All SCFAs stock solutions were prepared in 50% acetonitrile and stored at −20 °C. LC-MS grade acetonitrile, HPLC-grade acetonitrile, HPLC-grade methanol, and formic acid were obtained from J.T. Baker. Ultra-pure water (Mili-Q water) was produced using a water purification system (Mili-Q, Millipore, Milford, MA, USA).
3
Synthesis of Propofol-Fatty Acid Conjugates
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The raw materials needed for the synthesis of propofolfatty acid ester conjugates of selected four fatty acids, such as alpha-linolenic acid (ALA) , was extracted from eripupal oil according to a reported procedure 41) , valproic acid (VA) was purchased from Alfa-Aesar Chemicals (USA) . Butyric acid (BA) , 2-ethylhexanoic acid (2-EHA) , 2,4-diisopropylphenol, 2,6-diisopropylphenol and N,N -dicyclohexylcarbodiimide (DCC) , 4-(dimethyl amino) pyridine (DMAP) were purchased from Sigma-Aldrich Chemicals (USA) . All solvents and chemicals were of reagent grade and used directly without further purification. Silica gel (60-120 mesh) for column chromatography was purchased from Acme Synthetic Chemicals, Mumbai, India. Precoated TLC plates were purchased from Merck, Darmstadt, Germany. Human tumor cell lines were obtained from American Type Culture Collection, Manassas, VA, USA.
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